Rabbit Recombinant Monoclonal MYL3 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (AB108923), expandable thumbnail](https://content.abcam.com/products/images/myosin-light-chain-3-antibody-epr4160-ab108923--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118752.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (AB108923)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (AB108923), expandable thumbnail](https://content.abcam.com/products/images/myosin-light-chain-3-antibody-epr4160-ab108923--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img131586.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (AB108923)")
![Western blot - Anti-Myosin light chain 3 antibody [EPR4160] (AB108923), expandable thumbnail](https://content.abcam.com/products/images/myosin-light-chain-3-antibody-epr4160-ab108923--western-blot-img74454.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Myosin light chain 3 antibody [EPR4160] (AB108923)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (AB108923), expandable thumbnail](https://content.abcam.com/products/images/myosin-light-chain-3-antibody-epr4160-ab108923--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img122723.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (AB108923)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (AB108923), expandable thumbnail](https://content.abcam.com/products/images/myosin-light-chain-3-antibody-epr4160-ab108923--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img125928.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (AB108923)")
Publications
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- Neurourology and urodynamics 36:2011-20182017Periurethral muscle-derived mononuclear cell injection improves urethral sphincter restoration in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarcelo Pitelli Turco,Alex Balduino de Souza,Isida de Campos Sousa,Paula Fratini,Mariana Matera Veras,Marcio Nogueira Rodrigues,José de Bessa,Marina Pandolphi Brolio,Katia Ramos Moreira Leite,Homero Bruschini,Miguel Srougi,Maria Angélica Miglino,Cristiano Mendes GomesPubMed 28346707
- PloS one 9:e935832014Potential of adipose-derived mesenchymal stem cells and skeletal muscle-derived satellite cells for somatic cell nuclear transfer mediated transgenesis in Arbas Cashmere goats.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested | Not recommended |
| Mouse | Predicted | Not recommended | Not recommended | Predicted | Not recommended |
| Rat | Predicted | Not recommended | Not recommended | Predicted | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Regulatory light chain of myosin. Does not bind calcium.
Alternative names
Myosin light chain 3, Cardiac myosin light chain 1, Ventricular myosin alkali light chain, Ventricular myosin light chain 1, Ventricular/slow twitch myosin alkali light chain, CMLC1, MLC1SB, VLCl, MLC-lV/sb, MYL3
Recommended products
Rabbit Recombinant Monoclonal MYL3 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR4160
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Myosin light chain 3 (MLC3) also known as myosin regulatory light chain 3 is a part of the myosin protein complex. It plays a major role in muscle contraction by regulating the ATPase activity of myosin heads. MLC3 is encoded by the MYL3 gene and has a molecular mass of approximately 18kDa. It is expressed mainly in cardiac and skeletal muscle tissues. By interacting with myosin heavy chains MLC3 contributes to the force-generating capacity of muscles.
- Biological function summary
MLC3 functions as an essential component of the myosin light chain complex. This complex regulates contractile activity in muscle cells by modulating the interaction between actin and myosin chains. MLC3 activates the mechanical movement of myosin chains impacting cardiac and skeletal muscle contraction. When activated it affects the speed and strength of muscle contraction and it also has a role in muscle differentiation and development.
- Pathways
MLC3 is an integral part of the muscle contraction pathway. It interacts with proteins such as tropomyosin and troponin which are important for the regulation of muscle contraction. Additionally MLC3 participates in the calcium signaling pathway which is pivotal for contraction processes in muscles. This interaction helps to control the cytosolic calcium concentration a critical factor in muscle contraction dynamics.
- Associated diseases and disorders
MLC3 has connections to cardiomyopathy and hypertrophic conditions. Mutations or dysregulation of MYL3 can lead to cardiac hypertrophy a disease where the heart muscle thickens abnormally. MLC3's interaction with troponin and other myosin chains can exacerbate cardiac conditions when these pathways are disrupted. Studies have linked deficiencies in MLC3 to improper muscle development which may underline some muscle myopathies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923), expandable thumbnail](https://content.abcam.com/products/images/myosin-light-chain-3-antibody-epr4160-ab108923--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118752.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)
Immunohistochemical staining of paraffin-embedded human cardiac muscle using ab108923 at a dilution of 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923), expandable thumbnail](https://content.abcam.com/products/images/myosin-light-chain-3-antibody-epr4160-ab108923--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img131586.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)
Immunohistochemical staining of paraffin-embedded human skeletal muscle tissue using ab108923 at a diltion of 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Western blot - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923), expandable thumbnail](https://content.abcam.com/products/images/myosin-light-chain-3-antibody-epr4160-ab108923--western-blot-img74454.jpg "Western blot - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)")
Western blot - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)
All lanes: Western blot - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923) at 1/1000 dilution
Lane 1: human heart muscle tissue lysate at 10 µg/mL
Lane 2: human skeletal muscle tissue lysate at 10 µg/mL
Predicted band size: 21 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923), expandable thumbnail](https://content.abcam.com/products/images/myosin-light-chain-3-antibody-epr4160-ab108923--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img122723.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)
ab108923 showing negative staining in Normal brain tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923), expandable thumbnail](https://content.abcam.com/products/images/myosin-light-chain-3-antibody-epr4160-ab108923--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img125928.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)
ab108923 showing negative staining in Normal liver tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923), expandable thumbnail](https://content.abcam.com/products/images/myosin-light-chain-3-antibody-epr4160-ab108923--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img110548.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)
ab108923 showing negative staining in Normal tonsil tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923), expandable thumbnail](https://content.abcam.com/products/images/myosin-light-chain-3-antibody-epr4160-ab108923--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107314.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)
ab108923 showing negative staining in Normal breast tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923), expandable thumbnail](https://content.abcam.com/products/images/myosin-light-chain-3-antibody-epr4160-ab108923--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img110548.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923), expandable thumbnail](https://content.abcam.com/products/images/myosin-light-chain-3-antibody-epr4160-ab108923--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107314.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin light chain 3 antibody [EPR4160] (ab108923)")
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- Visit the general protocols
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