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AB108516

Anti-Myosin light chain 3 antibody [EPR4161]

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Rabbit Recombinant Monoclonal MYL3 antibody. Suitable for WB and reacts with Rat, Mouse, Human samples.

View Alternative Names

Myosin light chain 3, Cardiac myosin light chain 1, Ventricular myosin alkali light chain, Ventricular myosin light chain 1, Ventricular/slow twitch myosin alkali light chain, CMLC1, MLC1SB, VLCl, MLC-lV/sb, MYL3

1 Images
Western blot - Anti-Myosin light chain 3 antibody [EPR4161] (AB108516)
  • WB

Unknown

Western blot - Anti-Myosin light chain 3 antibody [EPR4161] (AB108516)

All lanes:

Western blot - Anti-Myosin light chain 3 antibody [EPR4161] (ab108516) at 1/1000 dilution

Lane 1:

Human skeletal muscle lysate at 10 µg

Lane 2:

Human heart lysate at 10 µg

Lane 3:

Mouse heart lysate at 10 µg

Lane 4:

Mouse liver lysate at 10 µg

Lane 5:

Rat heart lysate at 10 µg

Predicted band size: 21 kDa

false

  • Carrier free

    Anti-Myosin light chain 3 antibody [EPR4161] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4161

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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You may be interested in:

AB246538

Human Myosin light chain 3 ELISA Kit

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View product

We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Myosin light chain 3 (MLC3) also known as myosin regulatory light chain 3 is a part of the myosin protein complex. It plays a major role in muscle contraction by regulating the ATPase activity of myosin heads. MLC3 is encoded by the MYL3 gene and has a molecular mass of approximately 18kDa. It is expressed mainly in cardiac and skeletal muscle tissues. By interacting with myosin heavy chains MLC3 contributes to the force-generating capacity of muscles.
Biological function summary

MLC3 functions as an essential component of the myosin light chain complex. This complex regulates contractile activity in muscle cells by modulating the interaction between actin and myosin chains. MLC3 activates the mechanical movement of myosin chains impacting cardiac and skeletal muscle contraction. When activated it affects the speed and strength of muscle contraction and it also has a role in muscle differentiation and development.

Pathways

MLC3 is an integral part of the muscle contraction pathway. It interacts with proteins such as tropomyosin and troponin which are important for the regulation of muscle contraction. Additionally MLC3 participates in the calcium signaling pathway which is pivotal for contraction processes in muscles. This interaction helps to control the cytosolic calcium concentration a critical factor in muscle contraction dynamics.

MLC3 has connections to cardiomyopathy and hypertrophic conditions. Mutations or dysregulation of MYL3 can lead to cardiac hypertrophy a disease where the heart muscle thickens abnormally. MLC3's interaction with troponin and other myosin chains can exacerbate cardiac conditions when these pathways are disrupted. Studies have linked deficiencies in MLC3 to improper muscle development which may underline some muscle myopathies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulatory light chain of myosin. Does not bind calcium.
See full target information MYL3
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Product
Primary Antibodies

AB108923

Anti-Myosin light chain 3 antibody [EPR4160]

primary-antibodies

myosin-light-chain-3-antibody-epr4160-ab108923

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(0 reviews)

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