AB157747

Anti-Myosin light chain (phospho S1) antibody

Name: Anti-Myosin light chain (phospho S1) antibody (ab157747) | Abcam
Brand: Abcam Limited
SKU: AB157747

Be the first to review this product! Submit a review

(4 Publications)

Rabbit Polyclonal MYL9 phospho S1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Cow MYL9 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

MYRL2, MYL9, Myosin regulatory light polypeptide 9, Myosin regulatory light chain 9

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Myosin light chain (phospho S1) antibody (AB157747)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Myosin light chain (phospho S1) antibody (AB157747)

Immunofluorescent analysis of paraformaldehyde-fixed A7r5 cells labeling Myosin light chain with ab157747 at 1/100 dilution (left). Cells were dual stained with mouse anti-Myosin light chain antibody (middle panel) and the overlay of staining patterns is shown to the right. Goat anti-mouse Dylight 488 and goat anti-rabbit Dylight 594 were used for detection of primary antibodies.

Unknown

Western blot - Anti-Myosin light chain (phospho S1) antibody (AB157747)

All lanes:

Western blot - Anti-Myosin light chain (phospho S1) antibody (ab157747)

Lane 1:

C2C12 cell lysate, untreated

Lane 2:

C2C12 cell lysate treated with Lambda phosphatase

Predicted band size: 20 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Cow MYL9 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q5E9E2

Specificity

The immunogen sequence is highly conserved in Human, rat and mouse MYL9, and in other smooth muscle and non-muscle Myosin light chains.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab157747 was affinity purified using phospho-Myosin light chain (Ser-1) peptide (without carrier).

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Myosin light chain often referred to as MLC or the light chain myosin plays an important role in muscle contraction by binding to myosin heavy chains. This protein has a molecular mass of approximately 20 kDa and exists in smooth and striated muscle tissues as well as some non-muscle cells. MLC participates in regulating muscle contraction by interacting with myosin head groups during the ATPase activity which influences the actin-myosin interactions essential for muscle contraction.

Biological function summary

Myosin light chain interaction with myosin heavy chains regulates muscle contraction and relaxation. This small protein is a component of the larger myosin II complex which functions by modulating the motor activity on actin filaments. The phosphorylation state of the myosin light chain is critical in controlling the binding affinity of myosin to actin therefore directly affecting muscle contraction and cellular movement. In smooth muscles its phosphorylation by myosin light chain kinase increases the myosin ATPase activity contributing to muscle contractility.

Pathways

The action of myosin light chain fits into essential signaling processes regulating muscle function and cell motility. One significant pathway is the Rho/Rho-kinase pathway where phosphorylation of the myosin light chain modulates the contraction of smooth muscle and influences cell shape changes. Additionally the calcium-Calmodulin-Myosin Light Chain Kinase pathway also affects its phosphorylation status impacting smooth muscle contraction. Proteins such as phosphomyosin and myosin phosphatase interact within these pathways reinforcing the functional versatility of myosin light chain.

Myosin light chain is linked to conditions such as asthma and hypertension. Altered phosphorylation levels of this protein can cause increased smooth muscle contraction which is a contributing factor in the pathogenesis of these diseases. Asthma involves hyperresponsiveness of airway smooth muscle while hypertension often features increased vascular smooth muscle tone. The protein is functionally connected to tension-generating mechanisms involving myosin phosphatase providing a therapeutic target in managing muscle tone abnormalities in these disorders.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion (By similarity). In myoblasts, may regulate PIEZO1-dependent cortical actomyosin assembly involved in myotube formation (By similarity).

See full target information MYL9 phospho S1

style

left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 20:367-386 PubMed38164187

2024

Rebalancing TGF-β/PGE breaks RT-induced immunosuppressive barriers by enhancing tumor-infiltrated dendritic cell homing.

Applications

Unspecified application

Species

Unspecified reactive species

Ziqi Zhou,Qianqian Zhou,Jing Zhao,Xiaorong Hou,Junfang Yan,Xiansong Sun,Zhiwei Yang,Jiabin Ma,Fuquan Zhang,Linsheng Zhan,Ke Hu

British journal of pharmacology 176:2808-2824 PubMed31093964

2019

Histidine-rich glycoprotein ameliorates endothelial barrier dysfunction through regulation of NF-κB and MAPK signal pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shangze Gao,Hidenori Wake,Yuan Gao,Dengli Wang,Shuji Mori,Keyue Liu,Kiyoshi Teshigawara,Hideo Takahashi,Masahiro Nishibori

Biology open 7: PubMed30545950

2018

Dynamic microtubules drive fibroblast spreading.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Tvorogova,Aleena Saidova,Tatiana Smirnova,Ivan Vorobjev

Frontiers in cellular neuroscience 12:489 PubMed30618636

2018

ROCK Inhibitor Y27632 Induced Morphological Shift and Enhanced Neurite Outgrowth-Promoting Property of Olfactory Ensheathing Cells via YAP-Dependent Up-Regulation of L1-CAM.

Applications

Unspecified application

Species

Unspecified reactive species

Yijian Li,Shujia Huo,Yajie Fang,Ting Zou,Xianliang Gu,Qin Tao,Haiwei Xu

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Myosin light chain (phospho S1) antibody

Immunocytochemistry/ Immunofluorescence - Anti-Myosin light chain (phospho S1) antibody (AB157747)

Western blot - Anti-Myosin light chain (phospho S1) antibody (AB157747)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise