Anti-Myosin heavy chain (MYH 1/2) antibody [A4.1025] - recombinant (ab37484)

Anti-Myosin (MYH 1/2) antibody A4.1025 is a mouse monoclonal antibody that is used to detect Myosin (MYH 1/2) in Western blot, IHC, ICC/IF. Suitable for Human, Mouse, Rat samples.

- Recognises ATP binding loop of myosin heavy chain skeletal and cardiac muscle
- Recombinant format ensures unrivaled batch-to-batch consistency
- Trusted since 2006

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin (MYH 1/2) antibody [A4.1025] (AB37484), expandable thumbnail

Publications

Key facts

Isotype: IgG2a
Host species: Mouse
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	ICC/IF	WB	IHC-Fr	IHC-P
Human	Expected	Tested	Expected	Tested
Mouse	Tested	Tested	Tested	Tested
Rat	Expected	Tested	Tested	Tested

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 3.228 µg/mL	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Rat, Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 0.1614-0.807 µg/mL	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 0.1614-0.807 µg/mL	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 0.1614-0.807 µg/mL	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 16.14 µg/mL	Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Species Rat	Dilution info 16.14 µg/mL	Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 0.807 µg/mL	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 0.807 µg/mL	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 0.807 µg/mL	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

See full target information MYH1

Function

Muscle contraction.

Additional Targets

MYH2

Alternative names

Myosin-1, Myosin heavy chain 1, Myosin heavy chain 2x, Myosin heavy chain IIx/d, MyHC-2x, MyHC-IIx/d, MYH1

Key facts

Isotype: IgG2a
Host species: Mouse
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: A4.1025
Purification technique: Affinity purification Protein A
Specificity: ATP binding loop of myosin heavy chain skeletal and cardiac muscle.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Product Specifications
Anti-Myosin (MYH 1/2) antibody [A4.1025] (ab37484) is a mouse recombinant monoclonal antibody and is validated for use in ICC/IF, IHC-Fr, IHC-P, WB in human, mouse, rat samples.
Anti-Myosin (MYH 1/2) antibody [A4.1025] (ab37484) specifically detects Myosin (UniProt ID: P12882; Molecular weight: 223kDa) and is sold in 100 µg and 1 mg selling sizes.

Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-Myosin (MYH 1/2) antibody [A4.1025] (ab37484) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.

Related Products
Antibody clone A4.1025 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 647 (ab315962, ab315963).

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin (MYH 1/2) antibody [A4.1025] (ab37484)
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling Myosin (MYH1/2) with ab37484 at 0.807 μg/ml, followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879). Cytoplasmic staining on human skeletal muscle. The section was incubated with ab37484 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Western blot - Anti-Myosin (MYH 1/2) antibody [A4.1025] (ab37484)
The extra bands under 223KDa were mainly caused by degradation (PMID: 3295257).
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Myosin (MYH 1/2) antibody [A4.1025] (ab37484) at 0.1614 µg/mL
All lanes: Human skeletal muscle tissue lysate at 20 µg
Secondary
All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 223 kDa
Observed band size: 223 kDa
Exposure time: 3s
Immunohistochemistry (Frozen sections) - Anti-Myosin (MYH 1/2) antibody [A4.1025] (ab37484)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse skeletal muscle tissue labeling Myosin (MYH1/2) with ab37484 at 16.14 μg/ml followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). Positive staining on mouse skeletal muscle is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunocytochemistry/ Immunofluorescence - Anti-Myosin (MYH 1/2) antibody [A4.1025] (ab37484)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C2C12 cells labelling Myosin (MYH1/2) with ab37484 at 3.228 μg/ml, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (2μg/ml) (Green). Confocal image showing cytoplasmic staining in differentiated (treated) C2C12 cells. Anti-beta Tubulin antibody [EPR16774] ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (2.5μg/ml), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) at a 1/500 dilution (4μg/ml) (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (2μg/ml).
Western blot - Anti-Myosin (MYH 1/2) antibody [A4.1025] (ab37484)
Exposure times: Lane 1: 3 secs; Lane 2: 1 sec.
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Myosin (MYH 1/2) antibody [A4.1025] (ab37484) at 0.807 µg/mL
Lane 1: Mouse skeletal muscle tissue lysate at 20 µg
Lane 2: Rat skeletal muscle tissue lysate at 20 µg
Secondary
All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 223 kDa
Observed band size: 223 kDa
Western blot - Anti-Myosin (MYH 1/2) antibody [A4.1025] (ab37484)
The expression profile observed is consistent with what has been described in the literature (PMID: 26010876).
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Myosin (MYH 1/2) antibody [A4.1025] (ab37484) at 0.807 µg/mL
Lane 1: C2C12 (mouse myoblasts) whole cell lysate at 20 µg
Lane 2: Differentiated C2C12 whole cell lysate at 20 µg
Secondary
All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 223 kDa
Observed band size: 223 kDa
Exposure time: 3s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin (MYH 1/2) antibody [A4.1025] (ab37484)
Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling Myosin (MYH1/2) with ab37484 at 0.807 μg/ml, followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879). Cytoplasmic staining on human cardiac muscle. The section was incubated with ab37484 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin (MYH 1/2) antibody [A4.1025] (ab37484)
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Myosin (MYH1/2) with ab37484 at 0.807 μg/ml, followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879). Cytoplasmic staining on mouse skeletal muscle. The section was incubated with ab37484 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin (MYH 1/2) antibody [A4.1025] (ab37484)
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling Myosin (MYH1/2) with ab37484 at 0.807 μg/ml, followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879). Cytoplasmic staining on rat skeletal muscle. The section was incubated with ab37484 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Frozen sections) - Anti-Myosin (MYH 1/2) antibody [A4.1025] (ab37484)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat skeletal muscle tissue labeling Myosin (MYH1/2) with ab37484 at 16.14 μg/ml followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). Positive staining on rat skeletal muscle is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).