Rabbit Polyclonal Myosin Phosphatase antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human PPP1R12A.
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- Cancer science 112:2739-27522021PP1 regulatory subunit NIPP1 regulates transcription of E2F1 target genes following DNA damage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShunsuke Hanaki,Makoto Habara,Takahiro Masaki,Keisuke Maeda,Yuki Sato,Makoto Nakanishi,Midori ShimadaPubMed 33939241
- Medicine 99:e200602020Leukocyte Rho kinase activity and serum cystatin C affect cardiovascular events in acute coronary syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Ma,Wenqin Dai,Yongbo Lin,Zhongyuan Zhang,Yunhong Pan,Hongyan Han,Haizhen Jia,Jun Peng,Jinhe Zhao,Liang XuPubMed 32664054
- Frontiers in oncology 9:5392019Offsetting Expression Profiles of Prognostic Markers in Prostate Tumor vs. Its Microenvironment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhenyu Jia et. al.PubMed 31316912
- Pathology, research and practice 215:1524642019HMGCS2 functions as a tumor suppressor and has a prognostic impact in prostate cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSong Wan,Ming Xi,Hai-Bo Zhao,Wei Hua,Yuan-Ling Liu,Yu-Lin Zhou,Yang-Jia Zhuo,Ze-Zhen Liu,Zhi-Duan Cai,Yue-Ping Wan,Wei-De ZhongPubMed 31176575
- Current molecular medicine 18:100-1082018Decreased Expression of MYPT1 Contributes to Tumor Angiogenesis and Poor Patient Prognosis in Human Prostate Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesY Liang,Y Zhuo,Z Lin,F Jiang,Q Dai,J Lu,W Dong,X Zhu,Z Han,W ZhongPubMed 29974831
- Experimental and therapeutic medicine 14:2221-22272017Expression of MYPT1, CPI-17 and MLC20 in ileum of neonatal mouse NEC model and its significance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYinyu Yin,Yiping Li,Jian Pan,Ruze Tang,Jie Zhu,Zhenfang Qin,Xiaobing Xu,Jian WangPubMed 28962146
- Experimental and therapeutic medicine 14:308-3162017Resveratrol enhances vascular reactivity in mice following lipopolysaccharide challenge via the RhoA-ROCK-MLCP pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXu-Qing Wang et. al.PubMed 28672931
- Current molecular medicine 13:205-192012ROCK1 & 2 perform overlapping and unique roles in angiogenesis and angiosarcoma tumor progression.Applications:WBReactive species:Unspecified reactive speciesJ Montalvo,C Spencer,A Hackathorn,K Masterjohn,A Perkins,C Doty,A Arumugam,P P Ongusaha,R Lakshmanaswamy,J K Liao,D C Mitchell,B A BryanPubMed 22934846
- The Journal of biological chemistry 287:16575-852012Tra2β protein is required for tissue-specific splicing of a smooth muscle myosin phosphatase targeting subunit alternative exon.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human PPP1R12A. The exact immunogen used to generate this antibody is proprietary information. Database link O14974
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN-dependent suppression of HIF1A activity.
Alternative names
MBS, MYPT1, PPP1R12A, Protein phosphatase 1 regulatory subunit 12A, Myosin phosphatase-targeting subunit 1, Protein phosphatase myosin-binding subunit, Myosin phosphatase target subunit 1
Recommended products
Rabbit Polyclonal Myosin Phosphatase antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human PPP1R12A.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human PPP1R12A. The exact immunogen used to generate this antibody is proprietary information. Database link O14974
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Myosin phosphatase sometimes also known as Myosin Light Chain Phosphatase is an enzyme that plays a mechanical role in the dephosphorylation of myosin light chains which are important for muscle contraction and cell motility. This enzyme typically consists of three subunits: a catalytic subunit known as protein phosphatase 1 and two regulatory subunits. Together they generally create a structure with a mass between 100-150 kDa. You will find myosin phosphatase widely expressed across muscle tissues such as cardiac smooth and skeletal muscles.
- Biological function summary
Myosin phosphatase regulates contraction by reversing phosphorylation of myosin light chains an important process in muscle function and various cellular activities. This enzyme operates as part of the myosin phosphatase complex where it provides the capability needed to maintain equilibrium between phosphorylating and dephosphorylating processes. By controlling this balance myosin phosphatase plays a significant role in cell movement cell shape and tension maintenance.
- Pathways
Myosin phosphatase is integrated into the RhoA/Rho-kinase signaling pathway which is important for cytoskeletal dynamics and cell adhesion. Within this pathway myosin phosphatase interacts with proteins like Rho-kinase (ROCK) which counter-regulates its activity by inhibiting dephosphorylation. The crosstalk with another protein protein phosphatase 1 is also significant in controlling phosphorylation states and facilitating cellular responses to external stimuli.
- Associated diseases and disorders
Myosin phosphatase is involved in hypertension and heart failure through its action on smooth muscle contraction. Abnormal regulation and activity of this enzyme can lead to unwanted contraction and subsequently increased blood pressure. The protein Rho-kinase is implicated in these conditions as it often modifies the activity of myosin phosphatase. Furthermore deregulation of the associated cellular pathways contributes to cardiac stress and dysfunction highlighting its involvement in heart-related pathology.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-Myosin Phosphatase antibody (ab59235)
All lanes: Western blot - Anti-Myosin Phosphatase antibody (ab59235) at 1/500 dilution
Lane 1: NIH3T3 cell extract
Lane 2: NIH3T3 cell extract with immunising peptide
Predicted band size: 115 kDa
Observed band size: 130 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Phosphatase antibody (ab59235)
ab59235 at 1/50 dilution staining Myosin Phosphatase in human brain by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-Myosin Phosphatase antibody (ab59235)
Myosin Phosphatase western blot using anti-Myosin Phosphatase antibody ab59235. Publication image and figure legend from Montalvo, J., Spencer, C., et al., 2013, Curr Mol Med, PubMed 22934846.
ab59235 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab59235 please see the product overview.
ROCK1 & 2 display paralog specific cytoskeletal control in endothelial cells. (A) Growth patterns of confluent
monolayers of MS1 endothelial cells stably expressing either non-targeting control, ROCK1, or ROCK2 shRNA expression
vectors, or non-targeting control shRNA MS1 cells treated with 10 µM Y27632. (B) MS1 endothelial cells stably expressing non-targeting
control, ROCK1, or ROCK2 shRNA expression vectors, and control shRNA MS1 cells treated with 10 µM Y27632 were
grown on glass coverslips for 24 hours. Actin microfilaments in each condition were visualized by rhodamine-conjugated
phalloidin staining while nuclei were counterstained with DAPI. Immunofluorescent images were captured with scanning
confocal microscopy. (solid arrows=areas of cell retraction; a=control shRNA, b=Y27632, c= ROCK1 shRNA, d=ROCK2
shRNA) (C) Semi-quantitative RT-PCR analysis of actin alpha 1 (Acta1), actin alpha 2 (Acta2), and Gapdh steady state mRNA
expression levels. (D) Western blot detection of the phosphorylated (p) and total (t) forms of the myosin binding subunit of
myosin phosphatase (MBS), cofilin, and ezrin/radixin/moesin (ERM). Ponceau staining of the membrane was used as a loading
control. (E) Quantification of the normalized levels of phosphorylated MBS, cofilin, and ERM phosphorylation in each condition.
(C=control shRNA, R1=ROCK1 shRNA, R2=ROCK2 shRNA, Y=Y27632; * indicates p<0.05)
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