Anti-Myosin Phosphatase antibody

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Phosphatase antibody (AB59235)

ab59235 at 1/50 dilution staining Myosin Phosphatase in human brain by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.

• WB

Unknown

Western blot - Anti-Myosin Phosphatase antibody (AB59235)

All lanes:

Western blot - Anti-Myosin Phosphatase antibody (ab59235) at 1/500 dilution

Lane 1:

NIH3T3 cell extract

Lane 2:

NIH3T3 cell extract with immunising peptide

Predicted band size: 115 kDa

Observed band size: 130 kDa

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• WB

CiteAb

Western blot - Anti-Myosin Phosphatase antibody (AB59235)

Myosin Phosphatase western blot using anti-Myosin Phosphatase antibody ab59235. Publication image and figure legend from Montalvo, J., Spencer, C., et al., 2013, Curr Mol Med, PubMed 22934846.

ab59235 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab59235 please see the product overview.

ROCK1 & 2 display paralog specific cytoskeletal control in endothelial cells. (A) Growth patterns of confluent monolayers of MS1 endothelial cells stably expressing either non-targeting control, ROCK1, or ROCK2 shRNA expression vectors, or non-targeting control shRNA MS1 cells treated with 10 μM Y27632. (B) MS1 endothelial cells stably expressing non-targeting control, ROCK1, or ROCK2 shRNA expression vectors, and control shRNA MS1 cells treated with 10 μM Y27632 were grown on glass coverslips for 24 hours. Actin microfilaments in each condition were visualized by rhodamine-conjugated phalloidin staining while nuclei were counterstained with DAPI. Immunofluorescent images were captured with scanning confocal microscopy. (solid arrows=areas of cell retraction; a=control shRNA, b=Y27632, c= ROCK1 shRNA, d=ROCK2 shRNA) (C) Semi-quantitative RT-PCR analysis of actin alpha 1 (Acta1), actin alpha 2 (Acta2), and Gapdh steady state mRNA expression levels. (D) Western blot detection of the phosphorylated (p) and total (t) forms of the myosin binding subunit of myosin phosphatase (MBS), cofilin, and ezrin/radixin/moesin (ERM). Ponceau staining of the membrane was used as a loading control. (E) Quantification of the normalized levels of phosphorylated MBS, cofilin, and ERM phosphorylation in each condition. (C=control shRNA, R1=ROCK1 shRNA, R2=ROCK2 shRNA, Y=Y27632; * indicates p<0.05)

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