AB70809

Anti-Myosin Phosphatase antibody

Name: Anti-Myosin Phosphatase antibody (ab70809) | Abcam
Brand: Abcam Limited
SKU: AB70809
Rating: 5 (3 reviews)

(3 Reviews)

(3 Publications)

Rabbit Polyclonal Myosin Phosphatase antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human PPP1R12A.

View Alternative Names

MBS, MYPT1, PPP1R12A, Protein phosphatase 1 regulatory subunit 12A, Myosin phosphatase-targeting subunit 1, Protein phosphatase myosin-binding subunit, Myosin phosphatase target subunit 1

2 Images

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Immunoprecipitation - Anti-Myosin Phosphatase antibody (AB70809)

1mg whole lysate from HeLa cells was immunoprecipitated using ab70809 at 3ug/mg of lysate (lane 1) or a control rabbit Ig (lane 2). For the subsequent western blot, 20% of the immunoprecipitate was loaded per lane, and probed with ab70809 at 1ug/ml. Detection : chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-Myosin Phosphatase antibody (ab70809)

Predicted band size: 115 kDa

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Unknown

Western blot - Anti-Myosin Phosphatase antibody (AB70809)

All lanes:

Western blot - Anti-Myosin Phosphatase antibody (ab70809) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 115 kDa

Observed band size: 140 kDa

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Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human PPP1R12A. The exact immunogen used to generate this antibody is proprietary information.

O14974

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Myosin phosphatase sometimes also known as Myosin Light Chain Phosphatase is an enzyme that plays a mechanical role in the dephosphorylation of myosin light chains which are important for muscle contraction and cell motility. This enzyme typically consists of three subunits: a catalytic subunit known as protein phosphatase 1 and two regulatory subunits. Together they generally create a structure with a mass between 100-150 kDa. You will find myosin phosphatase widely expressed across muscle tissues such as cardiac smooth and skeletal muscles.

Biological function summary

Myosin phosphatase regulates contraction by reversing phosphorylation of myosin light chains an important process in muscle function and various cellular activities. This enzyme operates as part of the myosin phosphatase complex where it provides the capability needed to maintain equilibrium between phosphorylating and dephosphorylating processes. By controlling this balance myosin phosphatase plays a significant role in cell movement cell shape and tension maintenance.

Pathways

Myosin phosphatase is integrated into the RhoA/Rho-kinase signaling pathway which is important for cytoskeletal dynamics and cell adhesion. Within this pathway myosin phosphatase interacts with proteins like Rho-kinase (ROCK) which counter-regulates its activity by inhibiting dephosphorylation. The crosstalk with another protein protein phosphatase 1 is also significant in controlling phosphorylation states and facilitating cellular responses to external stimuli.

Myosin phosphatase is involved in hypertension and heart failure through its action on smooth muscle contraction. Abnormal regulation and activity of this enzyme can lead to unwanted contraction and subsequently increased blood pressure. The protein Rho-kinase is implicated in these conditions as it often modifies the activity of myosin phosphatase. Furthermore deregulation of the associated cellular pathways contributes to cardiac stress and dysfunction highlighting its involvement in heart-related pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN-dependent suppression of HIF1A activity.

See full target information PPP1R12A

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature microbiology 6:1031-1042 PubMed34282309

2021

MX2-mediated innate immunity against HIV-1 is regulated by serine phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Gilberto Betancor,Jose M Jimenez-Guardeño,Steven Lynham,Robin Antrobus,Hataf Khan,Andrew Sobala,Matthew D J Dicks,Michael H Malim

Scientific reports 6:33605 PubMed27646849

2016

Identification of antisense long noncoding RNAs that function as SINEUPs in human cells.

Applications

Unspecified application

Species

Unspecified reactive species

Aleks Schein,Silvia Zucchelli,Sakari Kauppinen,Stefano Gustincich,Piero Carninci

Scientific reports 6:31076 PubMed27484028

2016

Decompensated liver cirrhosis and neural regulation of mesenteric vascular tone in rats: role of sympathetic, nitrergic and sensory innervations.

Applications

Species

Rat

Esther Sastre,Laura Caracuel,Isabel Prieto,Pablo Llévenes,M Ángeles Aller,Jaime Arias,Gloria Balfagón,Javier Blanco-Rivero

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Myosin Phosphatase antibody

Immunoprecipitation - Anti-Myosin Phosphatase antibody (AB70809)

Western blot - Anti-Myosin Phosphatase antibody (AB70809)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise