Rabbit Polyclonal Myosin Phosphatase phospho T853 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human PPP1R12A phospho T853.
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Publications
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- Medicine 99:e200602020Leukocyte Rho kinase activity and serum cystatin C affect cardiovascular events in acute coronary syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Ma,Wenqin Dai,Yongbo Lin,Zhongyuan Zhang,Yunhong Pan,Hongyan Han,Haizhen Jia,Jun Peng,Jinhe Zhao,Liang XuPubMed 32664054
- Chemico-biological interactions 311:1087492019Protective effects of 18β-glycyrrhetinic acid on pulmonary arterial hypertension via regulation of Rho A/Rho kinsase pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMin Zhang et. al.PubMed 31325423
- Bioscience reports 38:2018Cyclic Mechanical Stretch Up-regulates Hepatoma-Derived Growth Factor Expression in Cultured Rat Aortic Smooth Muscle Cells.Applications:WBReactive species:RatYing-Hsien Kao,Po-Han Chen,Cheuk-Kwan Sun,Yo-Chen Chang,Yu-Chun Lin,Ming-Shian Tsai,Po-Huang Lee,Cheng-I ChengPubMed 29467272
- Experimental and therapeutic medicine 14:308-3162017Resveratrol enhances vascular reactivity in mice following lipopolysaccharide challenge via the RhoA-ROCK-MLCP pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXu-Qing Wang et. al.PubMed 28672931
- BioMed research international 2014:2145872014The prognostic values of leukocyte Rho kinase activity in acute ischemic stroke.
- Current molecular medicine 13:205-192012ROCK1 & 2 perform overlapping and unique roles in angiogenesis and angiosarcoma tumor progression.Applications:WBReactive species:Unspecified reactive speciesJ Montalvo,C Spencer,A Hackathorn,K Masterjohn,A Perkins,C Doty,A Arumugam,P P Ongusaha,R Lakshmanaswamy,J K Liao,D C Mitchell,B A BryanPubMed 22934846
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human PPP1R12A phospho T853. The exact immunogen used to generate this antibody is proprietary information. Database link O14974
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN-dependent suppression of HIF1A activity.
Alternative names
MBS, MYPT1, PPP1R12A, Protein phosphatase 1 regulatory subunit 12A, Myosin phosphatase-targeting subunit 1, Protein phosphatase myosin-binding subunit, Myosin phosphatase target subunit 1
Recommended products
Rabbit Polyclonal Myosin Phosphatase phospho T853 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human PPP1R12A phospho T853.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human PPP1R12A phospho T853. The exact immunogen used to generate this antibody is proprietary information. Database link O14974
- Purification technique
- Affinity purification Immunogen
- Specificity
Detects human Myosin Phosphatase only when phosphorylated at threonine 853 (threonine 852 for mouse and threonine 855 for rat)
- Concentration
- Purification notes
Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Myosin phosphatase sometimes also known as Myosin Light Chain Phosphatase is an enzyme that plays a mechanical role in the dephosphorylation of myosin light chains which are important for muscle contraction and cell motility. This enzyme typically consists of three subunits: a catalytic subunit known as protein phosphatase 1 and two regulatory subunits. Together they generally create a structure with a mass between 100-150 kDa. You will find myosin phosphatase widely expressed across muscle tissues such as cardiac smooth and skeletal muscles.
- Biological function summary
Myosin phosphatase regulates contraction by reversing phosphorylation of myosin light chains an important process in muscle function and various cellular activities. This enzyme operates as part of the myosin phosphatase complex where it provides the capability needed to maintain equilibrium between phosphorylating and dephosphorylating processes. By controlling this balance myosin phosphatase plays a significant role in cell movement cell shape and tension maintenance.
- Pathways
Myosin phosphatase is integrated into the RhoA/Rho-kinase signaling pathway which is important for cytoskeletal dynamics and cell adhesion. Within this pathway myosin phosphatase interacts with proteins like Rho-kinase (ROCK) which counter-regulates its activity by inhibiting dephosphorylation. The crosstalk with another protein protein phosphatase 1 is also significant in controlling phosphorylation states and facilitating cellular responses to external stimuli.
- Associated diseases and disorders
Myosin phosphatase is involved in hypertension and heart failure through its action on smooth muscle contraction. Abnormal regulation and activity of this enzyme can lead to unwanted contraction and subsequently increased blood pressure. The protein Rho-kinase is implicated in these conditions as it often modifies the activity of myosin phosphatase. Furthermore deregulation of the associated cellular pathways contributes to cardiac stress and dysfunction highlighting its involvement in heart-related pathology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-Myosin Phosphatase (phospho T853) antibody (ab59203)
All lanes: Western blot - Anti-Myosin Phosphatase (phospho T853) antibody (ab59203) at 1/500 dilution
Lane 1: NIH/3T3 cell extract
Lane 2: NIH/3T3 cell extract with immunizing phosphopeptide
Predicted band size: 115 kDa
Observed band size: 130 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Phosphatase (phospho T853) antibody (ab59203)
Immunohistochemical analysis of paraffin embedded human colon carcinoma tissue using ab59203 at 1/50 dilution. Samples were treated -/+ phosphopeptide.
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