AB59203

Anti-Myosin Phosphatase (phospho T853) antibody

Name: Anti-Myosin Phosphatase (phospho T853) antibody (ab59203) | Abcam
Brand: Abcam Limited
SKU: AB59203
Price: 545 USD
Availability: InStock
Rating: 1 (1 reviews)

(1 Review)

(8 Publications)

Rabbit Polyclonal Myosin Phosphatase phospho T853 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human PPP1R12A phospho T853.

View Alternative Names

MBS, MYPT1, PPP1R12A, Protein phosphatase 1 regulatory subunit 12A, Myosin phosphatase-targeting subunit 1, Protein phosphatase myosin-binding subunit, Myosin phosphatase target subunit 1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Phosphatase (phospho T853) antibody (AB59203)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Phosphatase (phospho T853) antibody (AB59203)

Immunohistochemical analysis of paraffin embedded human colon carcinoma tissue using ab59203 at 1/50 dilution. Samples were treated -/+ phosphopeptide.

Unknown

Western blot - Anti-Myosin Phosphatase (phospho T853) antibody (AB59203)

All lanes:

Western blot - Anti-Myosin Phosphatase (phospho T853) antibody (ab59203) at 1/500 dilution

Lane 1:

NIH/3T3 cell extract

Lane 2:

NIH/3T3 cell extract with immunizing phosphopeptide

Predicted band size: 115 kDa

Observed band size: 130 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human PPP1R12A phospho T853. The exact immunogen used to generate this antibody is proprietary information.

O14974

Specificity

Detects human Myosin Phosphatase only when phosphorylated at threonine 853 (threonine 852 for mouse and threonine 855 for rat)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Storage buffer

pH: 7 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Myosin phosphatase sometimes also known as Myosin Light Chain Phosphatase is an enzyme that plays a mechanical role in the dephosphorylation of myosin light chains which are important for muscle contraction and cell motility. This enzyme typically consists of three subunits: a catalytic subunit known as protein phosphatase 1 and two regulatory subunits. Together they generally create a structure with a mass between 100-150 kDa. You will find myosin phosphatase widely expressed across muscle tissues such as cardiac smooth and skeletal muscles.

Biological function summary

Myosin phosphatase regulates contraction by reversing phosphorylation of myosin light chains an important process in muscle function and various cellular activities. This enzyme operates as part of the myosin phosphatase complex where it provides the capability needed to maintain equilibrium between phosphorylating and dephosphorylating processes. By controlling this balance myosin phosphatase plays a significant role in cell movement cell shape and tension maintenance.

Pathways

Myosin phosphatase is integrated into the RhoA/Rho-kinase signaling pathway which is important for cytoskeletal dynamics and cell adhesion. Within this pathway myosin phosphatase interacts with proteins like Rho-kinase (ROCK) which counter-regulates its activity by inhibiting dephosphorylation. The crosstalk with another protein protein phosphatase 1 is also significant in controlling phosphorylation states and facilitating cellular responses to external stimuli.

Myosin phosphatase is involved in hypertension and heart failure through its action on smooth muscle contraction. Abnormal regulation and activity of this enzyme can lead to unwanted contraction and subsequently increased blood pressure. The protein Rho-kinase is implicated in these conditions as it often modifies the activity of myosin phosphatase. Furthermore deregulation of the associated cellular pathways contributes to cardiac stress and dysfunction highlighting its involvement in heart-related pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN-dependent suppression of HIF1A activity.

See full target information PPP1R12A phospho T853

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Diabetology & metabolic syndrome 17:374 PubMed41044623

2025

THBS1 regulates the function and insulin sensitivity of HTR8/SVneo cells treated with high glucose through the RhoA/ROCK1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yuerong Chai,Jie Tang,Aimin Liu,Qin Zhou

Lung 203:39 PubMed40047936

2025

FLRT3 Overexpression Attenuates Ischemia-Reperfusion Induced Vascular Hyperpermeability and Lung Injury Through RND3.

Applications

Unspecified application

Species

Unspecified reactive species

Yongmei Cao,Shiyang Sheng,Yong Zhong,Jiawei Shang,Cui Jin,Qin Tan,Feng Ping,Weifeng Huang,Yongchao Liu,Yingchuan Li

Medicine 99:e20060 PubMed32664054

2020

Leukocyte Rho kinase activity and serum cystatin C affect cardiovascular events in acute coronary syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Li Ma,Wenqin Dai,Yongbo Lin,Zhongyuan Zhang,Yunhong Pan,Hongyan Han,Haizhen Jia,Jun Peng,Jinhe Zhao,Liang Xu

Chemico-biological interactions 311:108749 PubMed31325423

2019

Protective effects of 18β-glycyrrhetinic acid on pulmonary arterial hypertension via regulation of Rho A/Rho kinsase pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Min Zhang,Zhi Chang,Peng Zhang,Zhicheng Jing,Lin Yan,Jun Feng,Zhiqiang Hu,Qingbin Xu,Wei Zhou,Ping Ma,Yinju Hao,Ru Zhou

Bioscience reports 38: PubMed29467272

2018

Cyclic Mechanical Stretch Up-regulates Hepatoma-Derived Growth Factor Expression in Cultured Rat Aortic Smooth Muscle Cells.

Applications

Species

Rat

Ying-Hsien Kao,Po-Han Chen,Cheuk-Kwan Sun,Yo-Chen Chang,Yu-Chun Lin,Ming-Shian Tsai,Po-Huang Lee,Cheng-I Cheng

Experimental and therapeutic medicine 14:308-316 PubMed28672931

2017

Resveratrol enhances vascular reactivity in mice following lipopolysaccharide challenge via the RhoA-ROCK-MLCP pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xu-Qing Wang,Yu-Ping Zhang,Li-Min Zhang,Niu-Niu Feng,Ming-Zhu Zhang,Zi-Gang Zhao,Chun-Yu Niu

BioMed research international 2014:214587 PubMed24716192

2014

The prognostic values of leukocyte Rho kinase activity in acute ischemic stroke.

Applications

Species

Human

Cheng-I Cheng,Yu-Chun Lin,Tzu-Hsien Tsai,Hung-Sheng Lin,Chia-Wei Liou,Wen-Neng Chang,Cheng-Hsien Lu,Chun-Man Yuen,Hon-Kan Yip

Current molecular medicine 13:205-19 PubMed22934846

2012

ROCK1 & 2 perform overlapping and unique roles in angiogenesis and angiosarcoma tumor progression.

Applications

Species

Unspecified reactive species

J Montalvo,C Spencer,A Hackathorn,K Masterjohn,A Perkins,C Doty,A Arumugam,P P Ongusaha,R Lakshmanaswamy,J K Liao,D C Mitchell,B A Bryan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Myosin Phosphatase (phospho T853) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin Phosphatase (phospho T853) antibody (AB59203)

Western blot - Anti-Myosin Phosphatase (phospho T853) antibody (AB59203)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise