AB150386

Anti-Myosin VIIa/MYO7A antibody [EPR7497]

RabMAb
Recombinant
What is this?

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(12 Publications)

Anti-Myosin VIIa/MYO7A antibody [EPR7497] (ab150386) is a rabbit monoclonal antibody detecting Myosin VIIa/MYO7A in Western Blot, Flow Cytometry (Intra), ICC/IF. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

USH1B, MYO7A, Unconventional myosin-VIIa

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Myosin VIIa/MYO7A antibody [EPR7497] (AB150386)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Myosin VIIa/MYO7A antibody [EPR7497] (AB150386)

Immunofluorescence analysis of A97 cells labeling Myosin VIIa/MYO7A with ab150386 at a 1/100 dilution.

Flow Cytometry (Intracellular) - Anti-Myosin VIIa/MYO7A antibody [EPR7497] (AB150386)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Myosin VIIa/MYO7A antibody [EPR7497] (AB150386)

Intracellular flow cytometric analysis of permeabilized Y79 cells labeling Myosin VIIa/MYO7A with ab150386 at a 1/100 dilution.

Unknown

Western blot - Anti-Myosin VIIa/MYO7A antibody [EPR7497] (AB150386)

All lanes:

Western blot - Anti-Myosin VIIa/MYO7A antibody [EPR7497] (ab150386) at 1/1000 dilution

Lane 1:

Y79 (Human retinoblastoma cell line) cell lysate at 10 µg

Lane 2:

Rat testis tissue lysate at 10 µg

Lane 3:

Mouse testis tissue lysate at 10 µg

Secondary

All lanes:

HRP labelled goat-anti rabbit antibody at 1/2000 dilution

Predicted band size: 254 kDa

false

Carrier free

Anti-Myosin VIIa/MYO7A antibody [EPR7497] - BSA and Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Myosin VIIa/MYO7A antibody [EPR7497]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Myosin VIIa/MYO7A antibody [EPR7497]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Myosin VIIa/MYO7A antibody [EPR7497]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Myosin VIIa/MYO7A antibody [EPR7497]
660 APC

APC Anti-Myosin VIIa/MYO7A antibody [EPR7497]
578 PE

PE Anti-Myosin VIIa/MYO7A antibody [EPR7497]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7497

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

What is this antibody validated in?
Anti-Myosin VIIa/MYO7A antibody [EPR7497] (ab150386) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of Myosin VIIa/MYO7A?
Anti-Myosin VIIa/MYO7A [EPR7497] (ab150386) specifically detects a band for Myosin VIIa/MYO7A (UniProt: Q13402) at a molecular weight of 254kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR7497] also available for your convenience: ab150386, Carrier free - ab248957, APC - ab319262, PE - ab319410, Alexa Fluor® 488 - ab319544, Alexa Fluor® 647 - ab319657, Alexa Fluor® 594 - ab319761, Alexa Fluor® 555 - ab319903

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Myosin VIIa also known as MYO7A or myosin 7a is a motor protein that plays an important role in cellular transport and movement. It belongs to the myosin superfamily and has a molecular mass of approximately 250 kDa. This protein is widely expressed in sensory cells especially in the inner ear and the retina. In reproductive tissues it aids in sperm tail movements. The mechanical function of MYO7A involves ATP-dependent interactions with actin filaments leading to motor activity and cargo transport within cells.

Biological function summary

Myosin VIIa is essential for the maintenance of stereocilia structure and function in the sensory hair cells of the cochlea. It forms part of a multiprotein complex that stabilizes the actin filaments contributing to auditory signal transduction. Furthermore it is critical in the retinal pigment epithelium for normal visual processes. The protein ensures the correct intracellular localization of key components affecting cell signaling and environmental responsiveness.

Pathways

Myosin VIIa is involved in both auditory and visual signal pathways key for sensory perception. In the auditory pathway it interacts with harmonin and cadherin 23 which also play a part in mechanotransduction. In the visual pathway it affects signaling processes that rely on precise positioning of visual pigments. These interactions highlight its role in maintaining proper sensory functions in complex signaling pathways.

Mutations in MYO7A can lead to conditions such as Usher syndrome type 1B and nonsyndromic deafness. Usher syndrome is characterized by combined deafness and vision loss due to retinal degeneration. Non-syndromic hearing loss involves hearing impairment without other clinical disabilities. Both disorders demonstrate the critical importance of myosin VIIa in auditory and visual neurons. Connections between MYO7A and cadherin proteins further implicate these pathways in disease pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails bind to membranous compartments, which are then moved relative to actin filaments. In the retina, plays an important role in the renewal of the outer photoreceptor disks. Plays an important role in the distribution and migration of retinal pigment epithelial (RPE) melanosomes and phagosomes, and in the regulation of opsin transport in retinal photoreceptors. In the inner ear, plays an important role in differentiation, morphogenesis and organization of cochlear hair cell bundles. Involved in hair-cell vesicle trafficking of aminoglycosides, which are known to induce ototoxicity (By similarity). Motor protein that is a part of the functional network formed by USH1C, USH1G, CDH23 and MYO7A that mediates mechanotransduction in cochlear hair cells. Required for normal hearing.

See full target information MYO7A

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Antioxidants (Basel, Switzerland) 14: PubMed40563254

2025

Ferulic Acid Combines with Ascorbic Acid to Target MMP9 to Attenuate Cisplatin-Induced Ototoxicity Through the p38MAPK Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Guojun Yang,Na Hu,Jie Gao,Xinzhi Li,Bin Zhang,Ketao Ma

PloS one 20:e0320634 PubMed40198625

2025

Ptgds downregulation protect vestibular hair cells from aminoglycoside-induced vestibulotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Chen,Zhimin Zhao,Jinghong Han,Yue Zhang,Guohui Nie

Cell communication and signaling : CCS 23:121 PubMed40045370

2025

Impact of POU3F4 mutation on cochlear development and auditory function.

Applications

Unspecified application

Species

Unspecified reactive species

Jiong Dang,Panpan Bian,Chao Chen,Chi Chen,Wenqi Shan,Luhang Cai,Yong Li,Huan Tan,Baicheng Xu,Minxin Guan,Yufen Guo

Cell biology and toxicology 41:31 PubMed39820591

2025

Innovative treatment of age-related hearing loss using MSCs and EVs with Apelin.

Applications

Unspecified application

Species

Unspecified reactive species

Shengqun Xu,Dongliang Liu,Fang Zhang,Yuan Tian

International journal of molecular sciences 25: PubMed39201392

2024

TNF Induces Laminin-332-Encoding Genes in Endothelial Cells and Laminin-332 Promotes an Atherogenic Endothelial Phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Assim Hayderi,Mulugeta Melkie Zegeye,Sare Meydan,Allan Sirsjö,Ashok Kumar Kumawat,Liza U Ljungberg

Cell death & disease 14:605 PubMed37704645

2023

Intranasal delivery of NGF rescues hearing impairment in aged SAMP8 mice.

Applications

Unspecified application

Species

Unspecified reactive species

Vanessa Castelli,Michele d'Angelo,Francesca Zazzeroni,Davide Vecchiotti,Edoardo Alesse,Daria Capece,Laura Brandolini,Franca Cattani,Andrea Aramini,Marcello Allegretti,Annamaria Cimini

BMC pharmacology & toxicology 24:16 PubMed36882858

2023

miR-34a/DRP-1-mediated mitophagy participated in cisplatin-induced ototoxicity via increasing oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyan Wang,Hanqing Lin,Weibiao Kang,Lingfei Huang,Sisi Gong,Tao Zhang,Xiaotong Huang,Feinan He,Yongyi Ye,Yiyang Tang,Haiying Jia,Haidi Yang

Frontiers in molecular neuroscience 15:963083 PubMed35992197

2022

Pitavastatin protects against neomycin-induced ototoxicity through inhibition of endoplasmic reticulum stress.

Applications

Unspecified application

Species

Unspecified reactive species

Yunhao Wu,Wei Meng,Ming Guan,Xiaolong Zhao,Chen Zhang,Qiaojun Fang,Yuhua Zhang,Zihui Sun,Mingjing Cai,Dongdong Huang,Xuechun Yang,Yafeng Yu,Yong Cui,Shuangba He,Renjie Chai

Bioengineered 13:6729-6739 PubMed35246011

2022

Small activating RNA activation of ATOH1 promotes regeneration of human inner ear hair cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yong-Li Zhang,Moorim Kang,Jian-Cheng Wu,Meng-Yao Xie,Ruo-Yan Xue,Qi Tang,Hua Yang,Long-Cheng Li

International journal of molecular sciences 23: PubMed35163494

2022

Usher Syndrome Belongs to the Genetic Diseases Associated with Radiosensitivity: Influence of the ATM Protein Kinase.

Applications

Unspecified application

Species

Unspecified reactive species

Joëlle Al-Choboq,Mélanie L Ferlazzo,Laurène Sonzogni,Adeline Granzotto,Laura El-Nachef,Mira Maalouf,Elise Berthel,Nicolas Foray

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Myosin VIIa/MYO7A antibody [EPR7497]

Immunocytochemistry/ Immunofluorescence - Anti-Myosin VIIa/MYO7A antibody [EPR7497] (AB150386)

Flow Cytometry (Intracellular) - Anti-Myosin VIIa/MYO7A antibody [EPR7497] (AB150386)

Western blot - Anti-Myosin VIIa/MYO7A antibody [EPR7497] (AB150386)

Related conjugates and formulations (7)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

Product promise