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AB248957

Anti-Myosin VIIa/MYO7A antibody [EPR7497] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal MYO7A antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

USH1B, MYO7A, Unconventional myosin-VIIa

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Myosin VIIa/MYO7A antibody [EPR7497] - BSA and Azide free (AB248957)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Myosin VIIa/MYO7A antibody [EPR7497] - BSA and Azide free (AB248957)

This data was developed using ab150386, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of A97 cells labeling Myosin VIIa/MYO7A with ab150386 at a 1/100 dilution.

Flow Cytometry (Intracellular) - Anti-Myosin VIIa/MYO7A antibody [EPR7497] - BSA and Azide free (AB248957)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Myosin VIIa/MYO7A antibody [EPR7497] - BSA and Azide free (AB248957)

This data was developed using ab150386, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized Y79 cells labeling Myosin VIIa/MYO7A with ab150386 at a 1/100 dilution.

Western blot - Anti-Myosin VIIa/MYO7A antibody [EPR7497] - BSA and Azide free (AB248957)
  • WB

Unknown

Western blot - Anti-Myosin VIIa/MYO7A antibody [EPR7497] - BSA and Azide free (AB248957)

This data was developed using ab150386, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Myosin VIIa/MYO7A antibody [EPR7497] (<a href='/en-us/products/primary-antibodies/myosin-viia-myo7a-antibody-epr7497-ab150386'>ab150386</a>) at 1/1000 dilution

Lane 1:

Y79 (Human retinoblastoma cell line) cell lysate at 10 µg

Lane 2:

Rat testis tissue lysate at 10 µg

Lane 3:

Mouse testis tissue lysate at 10 µg

Secondary

All lanes:

HRP labelled goat-anti rabbit antibody at 1/2000 dilution

Predicted band size: 254 kDa

false

  • Unconjugated

    Anti-Myosin VIIa/MYO7A antibody [EPR7497]

  • 660 APC

    APC Anti-Myosin VIIa/MYO7A antibody [EPR7497]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Myosin VIIa/MYO7A antibody [EPR7497]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Myosin VIIa/MYO7A antibody [EPR7497]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Myosin VIIa/MYO7A antibody [EPR7497]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Myosin VIIa/MYO7A antibody [EPR7497]

  • 578 PE

    PE Anti-Myosin VIIa/MYO7A antibody [EPR7497]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7497

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt (Intra), ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Secondary Antibodies

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

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Primary Antibodies

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Primary Antibodies

AB150820

Anti-USH1G antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-USH1G antibody (AB150820)

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Reactivity data

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Product details

ab248957 is the carrier-free version of ab150386.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Myosin VIIa also known as MYO7A or myosin 7a is a motor protein that plays an important role in cellular transport and movement. It belongs to the myosin superfamily and has a molecular mass of approximately 250 kDa. This protein is widely expressed in sensory cells especially in the inner ear and the retina. In reproductive tissues it aids in sperm tail movements. The mechanical function of MYO7A involves ATP-dependent interactions with actin filaments leading to motor activity and cargo transport within cells.
Biological function summary

Myosin VIIa is essential for the maintenance of stereocilia structure and function in the sensory hair cells of the cochlea. It forms part of a multiprotein complex that stabilizes the actin filaments contributing to auditory signal transduction. Furthermore it is critical in the retinal pigment epithelium for normal visual processes. The protein ensures the correct intracellular localization of key components affecting cell signaling and environmental responsiveness.

Pathways

Myosin VIIa is involved in both auditory and visual signal pathways key for sensory perception. In the auditory pathway it interacts with harmonin and cadherin 23 which also play a part in mechanotransduction. In the visual pathway it affects signaling processes that rely on precise positioning of visual pigments. These interactions highlight its role in maintaining proper sensory functions in complex signaling pathways.

Mutations in MYO7A can lead to conditions such as Usher syndrome type 1B and nonsyndromic deafness. Usher syndrome is characterized by combined deafness and vision loss due to retinal degeneration. Non-syndromic hearing loss involves hearing impairment without other clinical disabilities. Both disorders demonstrate the critical importance of myosin VIIa in auditory and visual neurons. Connections between MYO7A and cadherin proteins further implicate these pathways in disease pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails bind to membranous compartments, which are then moved relative to actin filaments. In the retina, plays an important role in the renewal of the outer photoreceptor disks. Plays an important role in the distribution and migration of retinal pigment epithelial (RPE) melanosomes and phagosomes, and in the regulation of opsin transport in retinal photoreceptors. In the inner ear, plays an important role in differentiation, morphogenesis and organization of cochlear hair cell bundles. Involved in hair-cell vesicle trafficking of aminoglycosides, which are known to induce ototoxicity (By similarity). Motor protein that is a part of the functional network formed by USH1C, USH1G, CDH23 and MYO7A that mediates mechanotransduction in cochlear hair cells. Required for normal hearing.
See full target information MYO7A
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of personalized medicine 12: PubMed35207643

2022

Detyrosinated α-Tubulin, Vimentin and PD-L1 in Circulating Tumor Cells (CTCs) Isolated from Non-Small Cell Lung Cancer (NSCLC) Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Spyridoula D Katsarou,Ippokratis Messaritakis,Anastasia Voumvouraki,Stavros Kakavogiannis,Athanasios Κotsakis,Saad Alkahtani,Christos Stournaras,Stuart S Martin,Vassilis Georgoulias,Galatea Kallergi
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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