AB155984

Anti-Myosin VIIa/MYO7A antibody [EPR7498]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal MYO7A antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications.

View Alternative Names

USH1B, MYO7A, Unconventional myosin-VIIa

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Myosin VIIa/MYO7A antibody [EPR7498] (AB155984)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Myosin VIIa/MYO7A antibody [EPR7498] (AB155984)

Immunofluorescence analysis of Y79 cells staining Myosin VIIa/MYO7A with ab155984 at 1/100 dilution.

Unknown

Western blot - Anti-Myosin VIIa/MYO7A antibody [EPR7498] (AB155984)

All lanes:

Western blot - Anti-Myosin VIIa/MYO7A antibody [EPR7498] (ab155984) at 1/1000 dilution

All lanes:

Y79 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 254 kDa

false

Carrier free

Anti-Myosin VIIa/MYO7A antibody [EPR7498] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7498

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Myosin VIIa also known as MYO7A or myosin 7a is a motor protein that plays an important role in cellular transport and movement. It belongs to the myosin superfamily and has a molecular mass of approximately 250 kDa. This protein is widely expressed in sensory cells especially in the inner ear and the retina. In reproductive tissues it aids in sperm tail movements. The mechanical function of MYO7A involves ATP-dependent interactions with actin filaments leading to motor activity and cargo transport within cells.

Biological function summary

Myosin VIIa is essential for the maintenance of stereocilia structure and function in the sensory hair cells of the cochlea. It forms part of a multiprotein complex that stabilizes the actin filaments contributing to auditory signal transduction. Furthermore it is critical in the retinal pigment epithelium for normal visual processes. The protein ensures the correct intracellular localization of key components affecting cell signaling and environmental responsiveness.

Pathways

Myosin VIIa is involved in both auditory and visual signal pathways key for sensory perception. In the auditory pathway it interacts with harmonin and cadherin 23 which also play a part in mechanotransduction. In the visual pathway it affects signaling processes that rely on precise positioning of visual pigments. These interactions highlight its role in maintaining proper sensory functions in complex signaling pathways.

Mutations in MYO7A can lead to conditions such as Usher syndrome type 1B and nonsyndromic deafness. Usher syndrome is characterized by combined deafness and vision loss due to retinal degeneration. Non-syndromic hearing loss involves hearing impairment without other clinical disabilities. Both disorders demonstrate the critical importance of myosin VIIa in auditory and visual neurons. Connections between MYO7A and cadherin proteins further implicate these pathways in disease pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails bind to membranous compartments, which are then moved relative to actin filaments. In the retina, plays an important role in the renewal of the outer photoreceptor disks. Plays an important role in the distribution and migration of retinal pigment epithelial (RPE) melanosomes and phagosomes, and in the regulation of opsin transport in retinal photoreceptors. In the inner ear, plays an important role in differentiation, morphogenesis and organization of cochlear hair cell bundles. Involved in hair-cell vesicle trafficking of aminoglycosides, which are known to induce ototoxicity (By similarity). Motor protein that is a part of the functional network formed by USH1C, USH1G, CDH23 and MYO7A that mediates mechanotransduction in cochlear hair cells. Required for normal hearing.

See full target information MYO7A

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Methods in molecular biology (Clifton, N.J.) 2557:453-493 PubMed36512231

2022

Common Markers and Small Molecule Inhibitors in Golgi Studies.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Bui,Drew Stark,Jie Li,Jianchao Zhang,Yanzhuang Wang

Frontiers in molecular biosciences 8:806650 PubMed35096971

2022

Accumulated ROS Activates HIF-1α-Induced Glycolysis and Exerts a Protective Effect on Sensory Hair Cells Against Noise-Induced Damage.

Applications

Unspecified application

Species

Unspecified reactive species

Shuo Liang,Shuohui Dong,Wenwen Liu,Man Wang,Shanshan Tian,Yu Ai,Haibo Wang

Drug design, development and therapy 15:3443-3450 PubMed34413631

2021

Hearing Protection Outcomes of Analog Electrode Arrays Coated with Different Drug-Eluting Polymer Films Implanted into Guinea Pig Cochleae.

Applications

Unspecified application

Species

Unspecified reactive species

Yuyu Huang,Haoran Yu,Min Liang,Shule Hou,Junmin Chen,Fan Zhang,Xiayu Sun,Huan Jia,Jun Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Myosin VIIa/MYO7A antibody [EPR7498]

Immunocytochemistry/ Immunofluorescence - Anti-Myosin VIIa/MYO7A antibody [EPR7498] (AB155984)

Western blot - Anti-Myosin VIIa/MYO7A antibody [EPR7498] (AB155984)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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