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AB10149

Anti-MYRIP antibody

4

(1 Review)

|

(4 Publications)

Goat Polyclonal MYRIP antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human MYRIP aa 800 to C-terminus.

View Alternative Names

SLAC2C, MYRIP, Rab effector MyRIP, Exophilin-8, Myosin-VIIa- and Rab-interacting protein, Synaptotagmin-like protein lacking C2 domains C, SlaC2-c, Slp homolog lacking C2 domains c

1 Images
Western blot - Anti-MYRIP antibody (AB10149)
  • WB

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Western blot - Anti-MYRIP antibody (AB10149)

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Western blot - Anti-MYRIP antibody (ab10149) at 0.3 µg/mL

All lanes:

Human Amygdala lysates at 35 µg

Predicted band size: 96 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human MYRIP aa 800 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8NFW9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" } } }
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Product details

A molecular complex composed of Rab27A, MYRIP and myosin VIIa bridges retinal melanosomes to the actin cytoskeleton and thereby mediates the local trafficking of these organelles. The defect of this molecular complex is likely to account for the perinuclear mislocalization of the melanosomes observed in the retinal pigment epithelium cells of myosin VIIa-defective mice.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MYRIP also known as Myosin VIIA and Rab Interacting Protein plays a role in linking Rab27A to myosin motor proteins which is essential for the transportation of cellular components. It is a protein with a molecular mass of approximately 131 kDa. MYRIP expresses in various tissues including the retina where it supports the function of photoreceptor cells. The protein also shows presence in specialized secretory cells contributing to the regulation of secretory pathways.
Biological function summary

MYRIP facilitates the connection between specific motor proteins and vesicle-bound Rab proteins forming part of a complex responsible for vesicle movement along actin filaments. By acting as an adapter it links the actin cytoskeleton with membrane-bound organelles and vesicles influencing intracellular transport and distribution. Its role in these complexes indicates its involvement in cellular trafficking mechanisms necessary for maintaining cellular organization and function.

Pathways

MYRIP engages in the exocytosis pathway and melanosome transport. It interacts with Rab27A participating in the pathway that mediates the transport of melanosomes within cells. This interaction is key to controlling the delivery of pigment granules in melanocytes. Additionally the exocytosis pathway relies on MYRIP for effective secretion where it works alongside other proteins like Rab27B to manage vesicle docking and fusion at the plasma membrane.

MYRIP relates to conditions like Griscelli syndrome type 1 which involves a defect in the transport of melanosomes. Changes or malfunctions in MYRIP or its associated proteins including Rab27A disrupt normal pigment distribution leading to this type of disorder. Furthermore given its expression in the retina MYRIP also connects to visual impairments wherein its dysfunction could affect photoreceptor cell health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Rab effector protein involved in melanosome transport. Serves as link between melanosome-bound RAB27A and the motor proteins MYO5A and MYO7A. May link RAB27A-containing vesicles to actin filaments. Functions as a protein kinase A-anchoring protein (AKAP). May act as a scaffolding protein that links PKA to components of the exocytosis machinery, thus facilitating exocytosis, including insulin release (By similarity).
See full target information MYRIP
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

eLife 12: PubMed36803984

2023

Functional hierarchy among different Rab27 effectors involved in secretory granule exocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Kunli Zhao,Kohichi Matsunaga,Kouichi Mizuno,Hao Wang,Katsuhide Okunishi,Tetsuro Izumi

Journal of cell science 129:592-603 PubMed26675235

2015

Interaction between MyRIP and the actin cytoskeleton regulates Weibel-Palade body trafficking and exocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ianina L Conte,Nicola Hellen,Ruben Bierings,Gregory I Mashanov,Jean-Baptiste Manneville,Nikolai I Kiskin,Matthew J Hannah,Justin E Molloy,Tom Carter

Proceedings of the National Academy of Sciences of 106:14966-71 PubMed19706440

2009

TLR4-mediated expulsion of bacteria from infected bladder epithelial cells.

Applications

IP

Species

Human

Jeongmin Song,Brian L Bishop,Guojie Li,Richard Grady,Ann Stapleton,Soman N Abraham

Blood 113:5010-8 PubMed19270261

2009

Rab27a and MyRIP regulate the amount and multimeric state of VWF released from endothelial cells.

Applications

ICC/IF

Species

Human

Thomas D Nightingale,Krupa Pattni,Alistair N Hume,Miguel C Seabra,Daniel F Cutler
View all publications
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Product promise

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