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Rabbit Recombinant Monoclonal MYSM1 antibody. Carrier free. Suitable for WB, IP and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-MYSM1 antibody [EPR18657] - BSA and Azide free (AB251165), expandable thumbnail
  • Western blot - Anti-MYSM1 antibody [EPR18657] - BSA and Azide free (AB251165), expandable thumbnail
  • Western blot - Anti-MYSM1 antibody [EPR18657] - BSA and Azide free (AB251165), expandable thumbnail
  • Immunoprecipitation - Anti-MYSM1 antibody [EPR18657] - BSA and Azide free (AB251165), expandable thumbnail
  • Western blot - Anti-MYSM1 antibody [EPR18657] - BSA and Azide free (AB251165), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIP
Human
Tested
Tested
Mouse
Tested
Expected
Rat
Tested
Expected

Tested
Tested

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Metalloprotease with deubiquitinase activity that plays important regulator roles in hematopoietic stem cell function, blood cell production and immune response (PubMed:24062447, PubMed:26220525, PubMed:28115216). Participates in the normal programming of B-cell responses to antigen after the maturation process (By similarity). Within the cytoplasm, plays critical roles in the repression of innate immunity and autoimmunity (PubMed:33086059). Removes 'Lys-63'-linked polyubiquitins from TRAF3 and TRAF6 complexes (By similarity). Attenuates NOD2-mediated inflammation and tissue injury by promoting 'Lys-63'-linked deubiquitination of RIPK2 component (By similarity). Suppresses the CGAS-STING1 signaling pathway by cleaving STING1 'Lys-63'-linked ubiquitin chains (PubMed:33086059). In the nucleus, acts as a hematopoietic transcription regulator derepressing a range of genes essential for normal stem cell differentiation including EBF1 and PAX5 in B-cells, ID2 in NK-cell progenitor or FLT3 in dendritic cell precursors (PubMed:24062447). Deubiquitinates monoubiquitinated histone H2A, a specific tag for epigenetic transcriptional repression, leading to dissociation of histone H1 from the nucleosome (PubMed:17707232).

Alternative names

KIAA1915, MYSM1, Deubiquitinase MYSM1, 2A-DUB

Recommended products

Rabbit Recombinant Monoclonal MYSM1 antibody. Carrier free. Suitable for WB, IP and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR18657
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab251165 is the carrier-free version of Anti-MYSM1 antibody [EPR18657] ab193081.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

MYSM1 also known as Myb-like SWIRM and MPN domains 1 functions as a histone H2A deubiquitinase. This protein weighs approximately 83 kDa. MYSM1 is found in the nucleus and shows expression in various tissues including hematopoietic cells such as T cells and bone marrow. By removing ubiquitin from histone H2A MYSM1 influences chromatin structure and gene transcription.

Biological function summary

MYSM1 plays an important role in regulating gene expression during processes like hematopoiesis and immune development. It forms part of a chromatin remodeling complex that modulates transcriptional activity by altering nucleosome configuration. This function is essential for proper stem cell maintenance and differentiation by ensuring that transcriptionally active and inactive regions are appropriately maintained and accessed.

Pathways

MYSM1 participates in the ubiquitin-proteasome pathway and is involved in DNA damage response. It associates closely with proteins like TP53 where it aids in governing cell cycle progression and apoptosis. MYSM1 also interacts with histone modification machinery exerting effects on transcriptional silencing and activation particularly influencing genes associated with immune cell lineage commitment.

Associated diseases and disorders

MYSM1 impacts conditions such as bone marrow failure and immunodeficiency. It connects with the TP53 protein where mutations or dysregulation in this pathway can lead to hematological abnormalities and impaired immune function. MYSM1 deficiency has been implicated in defective T cell maturation and aberrant myeloid lineage decisions contributing to these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-MYSM1 antibody [EPR18657] - BSA and Azide free (ab251165), expandable thumbnail

    Western blot - Anti-MYSM1 antibody [EPR18657] - BSA and Azide free (ab251165)

    This data was developed using Anti-MYSM1 antibody [EPR18657] ab193081, the same antibody clone in a different buffer formulation.

    False colour image of Western blot: Anti-MYSM1 antibody [EPR18657] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-MYSM1 antibody [EPR18657] ab193081 was shown to bind specifically to MYSM1. A band was observed at 110 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in MYSM1 knockout cell line Human MYSM1 knockout HEK-293T cell line ab267297 (knockout cell lysate Human MYSM1 knockout HEK-293T cell lysate ab257548). To generate this image, wild-type and MYSM1 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.

    All lanes: Western blot - Anti-MYSM1 antibody [EPR18657] (Anti-MYSM1 antibody [EPR18657] ab193081) at 1/1000 dilution

    Lane 1: Wild-type HEK-293T cell lysate at 20 µg

    Lane 2: MYSM1 knockout HEK-293T cell lysate at 20 µg

    Lane 2: Western blot - Human MYSM1 knockout HEK-293T cell line (Human MYSM1 knockout HEK-293T cell line ab267297)

    Lane 3: HeLa cell lysate at 20 µg

    Lane 4: RT-4 cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 95 kDa

    Observed band size: 110 kDa

  • Western blot - Anti-MYSM1 antibody [EPR18657] - BSA and Azide free (ab251165), expandable thumbnail

    Western blot - Anti-MYSM1 antibody [EPR18657] - BSA and Azide free (ab251165)

    This data was developed using Anti-MYSM1 antibody [EPR18657] ab193081, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Two bands detected represent two isoforms.

    All lanes: Western blot - Anti-MYSM1 antibody [EPR18657] (Anti-MYSM1 antibody [EPR18657] ab193081) at 1/1000 dilution

    Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Lane 2: HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

    Lane 3: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

    Lane 4: NCCIT (Human pluripotent embryonic carcinoma cell line) whole cell lysate at 20 µg

    Lane 5: K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 95 kDa

    Observed band size: 66 kDa, 95 kDa

    Exposure time: 1min

  • Western blot - Anti-MYSM1 antibody [EPR18657] - BSA and Azide free (ab251165), expandable thumbnail

    Western blot - Anti-MYSM1 antibody [EPR18657] - BSA and Azide free (ab251165)

    This data was developed using Anti-MYSM1 antibody [EPR18657] ab193081, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-MYSM1 antibody [EPR18657] (Anti-MYSM1 antibody [EPR18657] ab193081) at 1/1000 dilution

    All lanes: mESC (Mouse embryonic stem cell line) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 95 kDa

    Observed band size: 95 kDa

    Exposure time: 8s

  • Immunoprecipitation - Anti-MYSM1 antibody [EPR18657] - BSA and Azide free (ab251165), expandable thumbnail

    Immunoprecipitation - Anti-MYSM1 antibody [EPR18657] - BSA and Azide free (ab251165)

    This data was developed using Anti-MYSM1 antibody [EPR18657] ab193081, the same antibody clone in a different buffer formulation.

    MYSM1 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with Anti-MYSM1 antibody [EPR18657] ab193081 at 1/100 dilution. Western blot was performed from the immunoprecipitate using Anti-MYSM1 antibody [EPR18657] ab193081 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1000 dilution.

    Lane 1: HeLa whole cell lysate 10μg (Input).

    Lane 2: Anti-MYSM1 antibody [EPR18657] ab193081 IP in HeLa whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-MYSM1 antibody [EPR18657] ab193081 in HeLa whole cell lysate.

    Predicted band size: 66kDa, 95kDa.

    Observed band size: 66kDa, 95kDa.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    Two bands detected represent two isoforms.

    All lanes: Immunoprecipitation - Anti-MYSM1 antibody [EPR18657] (Anti-MYSM1 antibody [EPR18657] ab193081)

    Developed using the ECL technique.

    Predicted band size: 95 kDa

    Observed band size: 66 kDa, 95 kDa

    Exposure time: 3min

  • Western blot - Anti-MYSM1 antibody [EPR18657] - BSA and Azide free (ab251165), expandable thumbnail

    Western blot - Anti-MYSM1 antibody [EPR18657] - BSA and Azide free (ab251165)

    This data was developed using Anti-MYSM1 antibody [EPR18657] ab193081, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-MYSM1 antibody [EPR18657] (Anti-MYSM1 antibody [EPR18657] ab193081) at 1/1000 dilution

    Lane 1: RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

    Lane 2: PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg

    Lane 3: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 95 kDa

    Observed band size: 95 kDa

    Exposure time: 3min

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Product protocols

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