JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB251682

Anti-Myt1/MTF1 antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal Myt1/MTF1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MYT1 aa 300-450.

View Alternative Names

KIAA0835, KIAA1050, MTF1, MYTI, PLPB1, MYT1, Myelin transcription factor 1, MyT1, Myelin transcription factor I, Proteolipid protein-binding protein, MyTI

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myt1/MTF1 antibody (AB251682)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myt1/MTF1 antibody (AB251682)

Paraffin-embedded human cerebellum tissue stained for Myt1/MTF1 using ab251682 at 1/50 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-Myt1/MTF1 antibody (AB251682)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Myt1/MTF1 antibody (AB251682)

PFA-fixed, Triton X-100 permeabilized SH-SY5Y (human neuroblastoma cell line from bone marrow) cells stained for Myt1/MTF1 (green) using ab251682 at 4 μg/ml in ICC/IF.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human MYT1 aa 300-450. The exact immunogen used to generate this antibody is proprietary information.

Q01538

style
left-column

Complementary Products

Primary Antibodies

AB236401

Anti-MTF1 antibody [OTI2F3]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTF1 antibody [OTI2F3] (AB236401)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB200387

Anti-PKMYT1 antibody

Western blot - Anti-PKMYT1 antibody (AB200387)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Myt1 also known as Membrane-associated tyrosine- and threonine-specific Cdc2-inhibitory kinase is a part of the Myt family of serine/threonine protein kinases. The molecular weight of Myt1 is approximately 58 kDa. Myt1 is expressed in several tissues with higher levels observed in the central nervous system. This protein is known for regulating cell cycle progression particularly during the G2/M transition by phosphorylating cyclin-dependent kinases.
Biological function summary

Myt1 plays a significant role in the control of cell cycle checkpoints. It forms part of a larger regulatory complex involving other kinases such as Wee1 to ensure proper cell division by preventing premature entry into mitosis. Myt1 achieves this by inactivating CDK1 through phosphorylation which is a critical step in maintaining cell cycle integrity in response to cellular stresses and DNA damage.

Pathways

Myt1 integrates into the cell cycle regulation pathway impacting the G2/M checkpoint pathways. It functions alongside Wee1 and related kinases to exert fine control over mitotic entry ensuring that any damaged DNA is repaired before the cell divides. Myt1's actions are important for proper cell cycle progression and maintaining genomic stability making it an important player in the processes that govern cellular proliferation.

Myt1 has associations with various cancers due to its role in controlling cell division. When Myt1 function is disrupted it can lead to unchecked cell proliferation and tumorigenesis. Studies also connect Myt1 with Neurodevelopmental Disorders as its expression patterns affect neuronal differentiation and development. In these contexts Myt1 interacts with proteins like Wee1 highlighting its involvement in pathological states where cell cycle dysregulation and improper development take place.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Binds to the promoter region of genes encoding proteolipid proteins of the central nervous system. May play a role in the development of neurons and oligodendroglia in the CNS. May regulate a critical transition point in oligodendrocyte lineage development by modulating oligodendrocyte progenitor proliferation relative to terminal differentiation and up-regulation of myelin gene transcription.
See full target information MYT1
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Neoplasia (New York, N.Y.) 44:100931 PubMed37647805

2023

Histone H3 K27M-mediated regulation of cancer cell stemness and differentiation in diffuse midline glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Monika Sharma,Ivana Barravecchia,Brian Magnuson,Sarah F Ferris,April Apfelbaum,Nneka E Mbah,Jeanette Cruz,Varunkumar Krishnamoorthy,Robert Teis,McKenzie Kauss,Carl Koschmann,Costas A Lyssiotis,Mats Ljungman,Stefanie Galban
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com