Anti-MZF1 antibody

• WB

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Western blot - Anti-MZF1 antibody (AB64866)

All lanes:

Western blot - Anti-MZF1 antibody (ab64866) at 1/500 dilution

Lane 1:

Extracts from 293 cells at 5 µg with no immunising peptide

Lane 2:

Extracts from K562 cells at 5 µg with no immunising peptide

Lane 3:

Extracts from K562 cells at 5 µg with immunising peptide

Predicted band size: 82 kDa

Observed band size: 82 kDa

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• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-MZF1 antibody (AB64866)

ICC/IF image of ab64866 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64866, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MZF1 antibody (AB64866)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab64866 at a dilution of 1/50 - 1/100. Left hand panel - no immunising peptide; right hand panel - immunising peptide.