Mouse Recombinant Monoclonal N Cadherin antibody. Carrier free. Suitable for IP, WB and reacts with Human, Rat, Mouse samples.
Images
![Western blot - Anti-N Cadherin antibody [32/N-Cadherin] - BSA and Azide free (AB280380), expandable thumbnail](https://content.abcam.com/products/images/n-cadherin-antibody-32-n-cadherin-bsa-and-azide-free-ab280380--western-blot-img8883.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-N Cadherin antibody [32/N-Cadherin] - BSA and Azide free (AB280380)")
![Western blot - Anti-N Cadherin antibody [32/N-Cadherin] - BSA and Azide free (AB280380), expandable thumbnail](https://content.abcam.com/products/images/n-cadherin-antibody-32-n-cadherin-bsa-and-azide-free-ab280380--western-blot-img35649.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-N Cadherin antibody [32/N-Cadherin] - BSA and Azide free (AB280380)")
![Western blot - Anti-N Cadherin antibody [32/N-Cadherin] - BSA and Azide free (AB280380), expandable thumbnail](https://content.abcam.com/products/images/n-cadherin-antibody-32-n-cadherin-bsa-and-azide-free-ab280380--western-blot-img32947.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-N Cadherin antibody [32/N-Cadherin] - BSA and Azide free (AB280380)")
![Immunoprecipitation - Anti-N Cadherin antibody [32/N-Cadherin] - BSA and Azide free (AB280380), expandable thumbnail](https://content.abcam.com/products/images/n-cadherin-antibody-32-n-cadherin-bsa-and-azide-free-ab280380--immunoprecipitation-img9142.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-N Cadherin antibody [32/N-Cadherin] - BSA and Azide free (AB280380)")
![Western blot - Anti-N Cadherin antibody [32/N-Cadherin] - BSA and Azide free (AB280380), expandable thumbnail](https://content.abcam.com/products/images/n-cadherin-antibody-32-n-cadherin-bsa-and-azide-free-ab280380--western-blot-img73394.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-N Cadherin antibody [32/N-Cadherin] - BSA and Azide free (AB280380)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
Select an application| IP | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Not recommended |
| Mouse | Not recommended | Tested | Not recommended |
| Rat | Expected | Tested | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Calcium-dependent cell adhesion protein; preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. Plays a role in cell-to-cell junction formation between pancreatic beta cells and neural crest stem (NCS) cells, promoting the formation of processes by NCS cells (By similarity). Required for proper neurite branching. Required for pre- and postsynaptic organization (By similarity). CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.
Alternative names
CD325, CDHN, NCAD, CDH2, Cadherin-2, CDw325, Neural cadherin, N-cadherin
Recommended products
Mouse Recombinant Monoclonal N Cadherin antibody. Carrier free. Suitable for IP, WB and reacts with Human, Rat, Mouse samples.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Carrier free
- Yes
- Clone number
- 32/N-Cadherin
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab280380 is the carrier-free version of Anti-N Cadherin antibody [32/N-Cadherin] ab280375.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
N-Cadherin also known as CDH2 or neuronal cadherin is a calcium-dependent cell adhesion protein. It is characterized by its role in mediating intercellular connections primarily through its presence on neurons fibroblasts and certain epithelial cells. N-Cadherin has an approximate mass of 130 kDa. The protein is integral in forming homophilic interactions where N-Cadherin molecules on adjacent cells interact to establish robust cell-cell adhesion.
- Biological function summary
N-Cadherin significantly influences cell-cell interaction and communication facilitating cellular adhesion and signal transduction. It is a vital component of adherens junctions contributing to tissue morphogenesis and stability. N-Cadherin interacts with other cytoplasmic proteins such as catenins forming a complex essential for linking the actin cytoskeleton to the cell membrane. This interaction affects cellular behaviors including migration and differentiation.
- Pathways
N-Cadherin plays a significant role in the neural development and epithelial-to-mesenchymal transition (EMT) pathways important for development and cancer progression. It engages with related proteins such as beta-catenin which helps transduce signals within these pathways. N-Cadherin's interactions within these pathways highlight its role in maintaining multicellular structure and signaling processes important for development and pathogenesis.
- Associated diseases and disorders
N-Cadherin is associated with cancer and heart disease. In cancer particularly in the context of the EMT N-Cadherin facilitates tumor progression and metastasis cooperating with proteins like Twist and Snail to promote these processes. In heart disease alterations in N-Cadherin expression and function can impact cardiac muscle integrity and syncytium highlighting potential interactions with connexin43 to maintain cardiac function. Understanding these associations provides insights into therapeutic targets for these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-N Cadherin antibody [32/N-Cadherin] - BSA and Azide free (ab280380), expandable thumbnail](https://content.abcam.com/products/images/n-cadherin-antibody-32-n-cadherin-bsa-and-azide-free-ab280380--western-blot-img8883.jpg "Western blot - Anti-N Cadherin antibody [32/N-Cadherin] - BSA and Azide free (ab280380)")
Western blot - Anti-N Cadherin antibody [32/N-Cadherin] - BSA and Azide free (ab280380)
This data was developed using Anti-N Cadherin antibody [32/N-Cadherin] ab280375, the same antibody clone in a different buffer formulation.
Blocking and Diluting buffer and concentration: Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS.
Lanes 1 - 2: Merged signal (red and green). Green - Anti-N Cadherin antibody [32/N-Cadherin] ab280375 observed at 130 kDa. Red - loading control Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit monoclonal [EPR16891] to GAPDH) observed at 36 kDa.
Lanes 1-2: Anti-N Cadherin antibody [32/N-Cadherin] ab280375 Anti-CDH2 antibody was shown to react with CDH2 in 293T cells in Western blot. Loss of signal was observed when CDH2 knockout sample was used. Wild-type and CDH2 knockout samples were subjected to SDS-PAGE.
Anti-N Cadherin antibody [32/N-Cadherin] ab280375 and Anti-GAPDH antibody [EPR16891] (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) were incubated at 4℃ overnight at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680CW) (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800RD) (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.All lanes: Western blot - Anti-N Cadherin antibody [32/N-Cadherin] (Anti-N Cadherin antibody [32/N-Cadherin] ab280375) at 1/1000 dilution
Lane 1: Wild-type 293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg
Lane 2: CDH2 knockout 293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Mouse IgG H&L (IRDye® 800CW) (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) at 1/10000 dilution
Predicted band size: 99 kDa
Observed band size: 140 kDa
![Western blot - Anti-N Cadherin antibody [32/N-Cadherin] - BSA and Azide free (ab280380), expandable thumbnail](https://content.abcam.com/products/images/n-cadherin-antibody-32-n-cadherin-bsa-and-azide-free-ab280380--western-blot-img35649.jpg "Western blot - Anti-N Cadherin antibody [32/N-Cadherin] - BSA and Azide free (ab280380)")
Western blot - Anti-N Cadherin antibody [32/N-Cadherin] - BSA and Azide free (ab280380)
This data was developed using Anti-N Cadherin antibody [32/N-Cadherin] ab280375, the same antibody clone in a different buffer formulation.
Blocking and Diluting buffer and concentration: 5% NFDM/TBST
Exposure time 26 seconds
Negative control: MCF7 (PMID: 9177902)
All lanes: Western blot - Anti-N Cadherin antibody [32/N-Cadherin] (Anti-N Cadherin antibody [32/N-Cadherin] ab280375) at 1/1000 dilution
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg
Lane 2: MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 10 µg
SecondaryAll lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 99 kDa
Observed band size: 140 kDa
![Western blot - Anti-N Cadherin antibody [32/N-Cadherin] - BSA and Azide free (ab280380), expandable thumbnail](https://content.abcam.com/products/images/n-cadherin-antibody-32-n-cadherin-bsa-and-azide-free-ab280380--western-blot-img32947.jpg "Western blot - Anti-N Cadherin antibody [32/N-Cadherin] - BSA and Azide free (ab280380)")
Western blot - Anti-N Cadherin antibody [32/N-Cadherin] - BSA and Azide free (ab280380)
This data was developed using Anti-N Cadherin antibody [32/N-Cadherin] ab280375, the same antibody clone in a different buffer formulation.
Blocking and Diluting buffer and concentration: 5% NFDM/TBST
Exposure time 15 seconds
Lanes 1-2 used the fresh lysates to minimize protein degradation.
All lanes: Western blot - Anti-N Cadherin antibody [32/N-Cadherin] (Anti-N Cadherin antibody [32/N-Cadherin] ab280375) at 1/5000 dilution
Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 10 µg
Lane 2: 293T (human embryonic kidney epithelial cell), whole cell lysate at 10 µg
SecondaryAll lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 99 kDa
Observed band size: 140 kDa
![Immunoprecipitation - Anti-N Cadherin antibody [32/N-Cadherin] - BSA and Azide free (ab280380), expandable thumbnail](https://content.abcam.com/products/images/n-cadherin-antibody-32-n-cadherin-bsa-and-azide-free-ab280380--immunoprecipitation-img9142.jpg "Immunoprecipitation - Anti-N Cadherin antibody [32/N-Cadherin] - BSA and Azide free (ab280380)")
Immunoprecipitation - Anti-N Cadherin antibody [32/N-Cadherin] - BSA and Azide free (ab280380)
This data was developed using Anti-N Cadherin antibody [32/N-Cadherin] ab280375 the same antibody clone in a different buffer formulation.
N Cadherin was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with Anti-N Cadherin antibody [32/N-Cadherin] ab280375 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-N Cadherin antibody [32/N-Cadherin] ab280375 at 1/1000 dilution. HRP-conjuagted mouse IgG for IP (Anti-mouse IgG for IP (HRP) ab131368) was used at 1/1000 dilution.
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10ug
Lane 2: Anti-N Cadherin antibody [32/N-Cadherin] ab280375 IP in HeLa whole cell lysate
Lane 3: Mouse monoclonal IgG1 (Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control ab18443) instead of Anti-N Cadherin antibody [32/N-Cadherin] ab280375 in HeLa whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 23 seconds
The molecular weight observed is consistent with what has been described in the literature (PMID: 22553038)
All lanes: Immunoprecipitation - Anti-N Cadherin antibody [32/N-Cadherin] (Anti-N Cadherin antibody [32/N-Cadherin] ab280375)
Predicted band size: 99 kDa
![Western blot - Anti-N Cadherin antibody [32/N-Cadherin] - BSA and Azide free (ab280380), expandable thumbnail](https://content.abcam.com/products/images/n-cadherin-antibody-32-n-cadherin-bsa-and-azide-free-ab280380--western-blot-img73394.jpg "Western blot - Anti-N Cadherin antibody [32/N-Cadherin] - BSA and Azide free (ab280380)")
Western blot - Anti-N Cadherin antibody [32/N-Cadherin] - BSA and Azide free (ab280380)
This data was developed using Anti-N Cadherin antibody [32/N-Cadherin] ab280375, the same antibody clone in a different buffer formulation.
Blocking and Diluting buffer and concentration: 5% NFDM/TBST
Exposure time Lanes 1-2: 15 seconds
Lanes 3: 10 seconds
Lanes 4-5: 26 seconds
All lanes: Western blot - Anti-N Cadherin antibody [32/N-Cadherin] (Anti-N Cadherin antibody [32/N-Cadherin] ab280375) at 1/1000 dilution
Lane 1: Mouse brain lysate at 10 µg
Lane 2: Mouse kidney lysate at 10 µg
Lane 3: Rat heart lysate at 10 µg
Lane 4: C6 (rat glial tumor glial cell), whole cell lysate at 10 µg
Lane 5: PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 10 µg
SecondaryAll lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 99 kDa
Observed band size: 140 kDa
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