Anti-N Cadherin antibody [5D5] - Intercellular Junction Marker

6 product images

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  Flow Cytometry - Anti-N Cadherin antibody [5D5] - Intercellular Junction Marker (ab98952)

  Overlay histogram showing HeLa cells stained with ab98952 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab98952, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.
  
  

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  Western blot - Anti-N Cadherin antibody [5D5] - Intercellular Junction Marker (ab98952)

  All lanes: Western blot - Anti-N Cadherin antibody [5D5] - Intercellular Junction Marker (ab98952) at 1/500 dilution

  Lane 1: A431 cell lysate at 15 µL

  Lane 2: NIH-3T3 cell lysate at 15 µL

  Lane 3: HeLa cell lysate at 15 µL

  Lane 4: C6 cell lysate at 15 µL

  Lane 5: LNCap cell lysate at 15 µL

  Secondary

  All lanes: HRP-Goat Anti-Mouse Igg (Fc) at 1/10000 dilution

  Predicted band size: 99 kDa

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  This image is courtesy of an anonymous customer review

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [5D5] - Intercellular Junction Marker (ab98952)

  ab98952 staining N Cadherin in human NSCLC tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with using HOPE technique and blocked for 5 minutes at 25°C. Samples were incubated with primary antibody (1/200) for 1 hour at 25°C. An undiluted HRP-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.

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  This image is courtesy of an anonymous customer review

  Immunocytochemistry/ Immunofluorescence - Anti-N Cadherin antibody [5D5] - Intercellular Junction Marker (ab98952)

  ab98952 staining N Cadherin in paraffin-embedded human lung cancer tissue by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with HOPE and blocked. Samples were incubated with primary antibody (1/200) for 1 hour at 25°C. A TRITC-conjugated goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [5D5] - Intercellular Junction Marker (ab98952)

  Immunohistochemical analysis of paraffin-embedded Human lung cancer (1), colon cancer (2), ovarian cancer (3) and mammary cancer (4), using ab98952 at 1/200 dilution with DAB staining.

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  Immunocytochemistry/ Immunofluorescence - Anti-N Cadherin antibody [5D5] - Intercellular Junction Marker (ab98952)

  Immunofluorescence analysis of A431 cells using ab98952 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye