AB19348

Anti-N Cadherin antibody [8C11]

Name: Anti-N Cadherin antibody [8C11] (ab19348) | Abcam
Brand: Abcam Limited
SKU: AB19348
Price: 530 USD
Availability: InStock
Rating: 4 (2 reviews)

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(2 Reviews)

(38 Publications)

Mouse Monoclonal N Cadherin antibody. Suitable for IHC-P, ICC/IF and reacts with Human, Bird, Rabbit, Hamster samples. Cited in 38 publications.

View Alternative Names

CD325, CDHN, NCAD, CDH2, Cadherin-2, CDw325, Neural cadherin, N-cadherin

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-N Cadherin antibody [8C11] (AB19348)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-N Cadherin antibody [8C11] (AB19348)

ab19348 staining N-Cadherin in SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells.

The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Tween for 5 mins and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab19348 at 5 μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (shown in green) and ab150084, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labeled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Anti-N Cadherin antibody [8C11] (AB19348)

Immunofluorescence analysis of ARPE-19 (human retinal pigment epithelial) cells, staining N Cadherin (green) with ab19348, at 1/20 dilution.

ARPE-19 monolayer cultures were fixed in paraformaldehyde, permeabilized with 0.2% Triton X-100 for 15 min and blocked with 2% BSA for 30 min. Samples were incubated with primary antibody for 16 hours at 4°C before incubation with an Alexa Fluor^® 488-conjugated donkey anti-mouse secondary IgG for 60 min.

Image from Chen HC et al., PLoS One. 2012;7(5):e36864. Epub 2012 May 9. Fig 1.; doi: 10.1371/journal.pone.0036864; May 9, 2012, PLoS One. 2012; 7(5): e36864. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [8C11] (AB19348)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [8C11] (AB19348)

IHC image of N cadherin staining in a section of formalin-fixed paraffin-embedded normal human heart performed on a Leica BOND^TM system using the standard protocol F.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab19348, 1 μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

The inset secondary-only control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Biotin

Biotin Anti-N Cadherin antibody [8C11]
578 PE

PE Anti-N Cadherin antibody [8C11]
Carrier free

Anti-N Cadherin antibody [8C11] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

8C11

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Human, Bird

Applications

IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Epitope

The 8C11 monoclonal binds to the extracellular domain of N-cadherin between EC3 and EC4 (PubMed ID: 12604612).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" }, "Bird": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" }, "Hamster": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" }, "Pig": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "5 µg/µL", "ICCIF-species-notes": "" } } }

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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

N-Cadherin also known as CDH2 or neuronal cadherin is a calcium-dependent cell adhesion protein. It is characterized by its role in mediating intercellular connections primarily through its presence on neurons fibroblasts and certain epithelial cells. N-Cadherin has an approximate mass of 130 kDa. The protein is integral in forming homophilic interactions where N-Cadherin molecules on adjacent cells interact to establish robust cell-cell adhesion.

Biological function summary

N-Cadherin significantly influences cell-cell interaction and communication facilitating cellular adhesion and signal transduction. It is a vital component of adherens junctions contributing to tissue morphogenesis and stability. N-Cadherin interacts with other cytoplasmic proteins such as catenins forming a complex essential for linking the actin cytoskeleton to the cell membrane. This interaction affects cellular behaviors including migration and differentiation.

Pathways

N-Cadherin plays a significant role in the neural development and epithelial-to-mesenchymal transition (EMT) pathways important for development and cancer progression. It engages with related proteins such as beta-catenin which helps transduce signals within these pathways. N-Cadherin's interactions within these pathways highlight its role in maintaining multicellular structure and signaling processes important for development and pathogenesis.

N-Cadherin is associated with cancer and heart disease. In cancer particularly in the context of the EMT N-Cadherin facilitates tumor progression and metastasis cooperating with proteins like Twist and Snail to promote these processes. In heart disease alterations in N-Cadherin expression and function can impact cardiac muscle integrity and syncytium highlighting potential interactions with connexin43 to maintain cardiac function. Understanding these associations provides insights into therapeutic targets for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium-dependent cell adhesion protein; preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone : upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. Plays a role in cell-to-cell junction formation between pancreatic beta cells and neural crest stem (NCS) cells, promoting the formation of processes by NCS cells (By similarity). Required for proper neurite branching. Required for pre- and postsynaptic organization (By similarity). CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. May promote axon outgrowth and motor fiber repair via DSP-mediated recruitment to outgrowth tips (By similarity).

See full target information CDH2

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Publications (38)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 23:574 PubMed40830966

2025

Bioengineered cardiovascular bypass grafts via in vivo self-assembly of scaffold-guided tubular tissue in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Shiqi Hu,Xiuhong Sun,Rui Zhou,Kai Fu,Yunfei Mo,Jialin Xu,Xiaoyan Shen,Shiwen Liu,Yuqing Niu

eLife 14: PubMed40810390

2025

Interplay of YEATS2 and GCDH regulates histone crotonylation and drives EMT in head and neck cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Deepak Pant,Parik Kakani,Rushikesh Joshi,Abin Sabu,Shruti Agrawal,Atul Samaiya,Sanjeev Shukla

Nature communications 16:5433 PubMed40595635

2025

SEMA3B switches axon-axon to axon-glia interactions required for unmyelinated axon envelopment and integrity.

Applications

Unspecified application

Species

Unspecified reactive species

Li Liu,Zhigang Gao,Xueyuan Niu,Hong Yu,Xiaolong Xin,Yanlan Gu,Genghai Ma,Yan Gu,Yijun Liu,Sanhua Fang,Till Marquardt,Liang Wang

Cell death discovery 11:115 PubMed40118855

2025

FDX1 overexpression inhibits the growth and metastasis of clear cell renal cell carcinoma by upregulating FMR1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Wuping Yang,Cunjin Wu,Chaochao Jiang,Taile Jing,Minghao Lu,Dan Xia,Ding Peng

Journal of translational medicine 23:335 PubMed40089779

2025

F-box protein FBXO21 overexpression inhibits the proliferation and metastasis of clear cell renal cell carcinoma and is closely related to the CREB pathway and tumor immune cell infiltration.

Applications

Unspecified application

Species

Unspecified reactive species

Wuping Yang,Taile Jing,Cunjin Wu,Minghao Lu,Xiaolin Yao,Dan Xia,Ding Peng

Cells, tissues, organs 213:446-463 PubMed38768571

2024

Cadherin Expression Is Regulated by Mechanical Phenotypes of Fibroblasts in the Perivascular Matrix.

Applications

Unspecified application

Species

Unspecified reactive species

Vaishali Bala,Vidhi Patel,Mary Kathryn Sewell-Loftin

The Journal of cell biology 223: PubMed38477830

2024

N-cadherin dynamically regulates pediatric glioma cell migration in complex environments.

Applications

Unspecified application

Species

Unspecified reactive species

Dayoung Kim,James M Olson,Jonathan A Cooper

iScience 26:108497 PubMed38213789

2024

FEZ1 participates in human embryonic brain development by modulating neuronal progenitor subpopulation specification and migrations.

Applications

Unspecified application

Species

Unspecified reactive species

Yinghua Qu,Jonathan Jun-Yong Lim,Omer An,Henry Yang,Yi-Chin Toh,John Jia En Chua

Cell death & disease 14:212 PubMed36966163

2023

ZNF582 overexpression restrains the progression of clear cell renal cell carcinoma by enhancing the binding of TJP2 and ERK2 and inhibiting ERK2 phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Wuping Yang,Zedan Zhang,Lei Li,Kenan Zhang,Yawei Xu,Mancheng Xia,Jingcheng Zhou,Yanqing Gong,Jinchao Chen,Kan Gong

Journal of assisted reproduction and genetics 40:201-210 PubMed36471201

2022

CircSTAM inhibits migration and invasion of trophoblast cells by regulating miR-148a-5p/PTEN axis.

Applications

Unspecified application

Species

Unspecified reactive species

Lingfeng Chen,Jinyu Yan,Haiyan Zhang,Jia Xu,Xiaopei Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-N Cadherin antibody [8C11]

Immunocytochemistry/ Immunofluorescence - Anti-N Cadherin antibody [8C11] (AB19348)

Immunocytochemistry/ Immunofluorescence - Anti-N Cadherin antibody [8C11] (AB19348)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [8C11] (AB19348)

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Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Epitope

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (38)

Product promise