AB211126

Anti-N Cadherin antibody

Name: Anti-N Cadherin antibody (ab211126) | Abcam
Brand: Abcam Limited
SKU: AB211126
Price: 495 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal N Cadherin antibody. Suitable for WB, IHC - Wmt and reacts with Zebrafish samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Zebrafish cdh2 aa 100-350.

View Alternative Names

pac, cdh2, Cadherin-2, Neural cadherin, Parachute, ZNCAD, N-cadherin

3 Images

IHC - Wholemount - Anti-N Cadherin antibody (AB211126)

IHC - Wmt

Supplier Data

IHC - Wholemount - Anti-N Cadherin antibody (AB211126)

Immunohistochemical analysis (wholemount) of 2 days-post-fertilization zebrafish embryo labeling N Cadherin with ab211126 at 1/50 dilution.

IHC - Wmt

Supplier Data

IHC - Wholemount - Anti-N Cadherin antibody (AB211126)

Immunohistochemical analysis (wholemount) of 2 days-post-fertilization zebrafish embryo labeling N Cadherin with ab211126 at 1/50 dilution.

Supplier Data

Western blot - Anti-N Cadherin antibody (AB211126)

7.5% SDS PAGE

All lanes:

Western blot - Anti-N Cadherin antibody (ab211126) at 1/1000 dilution

All lanes:

Zebrafish brain whole cell lysate at 30 µg

Predicted band size: 99 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Zebrafish

Applications

WB, IHC - Wmt

applications

Immunogen

Recombinant Fragment Protein within Zebrafish cdh2 aa 100-350. The exact immunogen used to generate this antibody is proprietary information.

Q90275

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCWmt" : {"fullname" : "IHC - Wholemount", "shortname":"IHC - Wmt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Zebrafish": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCWmt-species-checked": "testedAndGuaranteed", "IHCWmt-species-dilution-info": "1/50 - 1/500", "IHCWmt-species-notes": "<p></p>" } } }

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We’re constantly upgrading our range to offer the highest quality products. We have selected this alternative product for your review because it gives improved performance, is easier and more convenient to use, or is available faster.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

N-Cadherin also known as CDH2 or neuronal cadherin is a calcium-dependent cell adhesion protein. It is characterized by its role in mediating intercellular connections primarily through its presence on neurons fibroblasts and certain epithelial cells. N-Cadherin has an approximate mass of 130 kDa. The protein is integral in forming homophilic interactions where N-Cadherin molecules on adjacent cells interact to establish robust cell-cell adhesion.

Biological function summary

N-Cadherin significantly influences cell-cell interaction and communication facilitating cellular adhesion and signal transduction. It is a vital component of adherens junctions contributing to tissue morphogenesis and stability. N-Cadherin interacts with other cytoplasmic proteins such as catenins forming a complex essential for linking the actin cytoskeleton to the cell membrane. This interaction affects cellular behaviors including migration and differentiation.

Pathways

N-Cadherin plays a significant role in the neural development and epithelial-to-mesenchymal transition (EMT) pathways important for development and cancer progression. It engages with related proteins such as beta-catenin which helps transduce signals within these pathways. N-Cadherin's interactions within these pathways highlight its role in maintaining multicellular structure and signaling processes important for development and pathogenesis.

N-Cadherin is associated with cancer and heart disease. In cancer particularly in the context of the EMT N-Cadherin facilitates tumor progression and metastasis cooperating with proteins like Twist and Snail to promote these processes. In heart disease alterations in N-Cadherin expression and function can impact cardiac muscle integrity and syncytium highlighting potential interactions with connexin43 to maintain cardiac function. Understanding these associations provides insights into therapeutic targets for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium-dependent cell adhesion protein; preferentially mediates homotypic cell-cell adhesion (By similarity). Cadherins contribute to the sorting of heterogeneous cell types, and thereby play an important role during embryonic development (PubMed : 12091300). Required for normal neural tube morphogenesis (PubMed : 12091300, PubMed : 21115806). Required to allow mid- and hindbrain neurons to reach and maintain their appropriate positions within the neural tube by restricting neuronal motility within the embryonic mid- and hindbrain (PubMed : 14648850, PubMed : 21115806). Functions with protocadherin pcdh19 to coordinate cell adhesion and cell movements during neurulation (PubMed : 21115806). pcdh19 shows little cell adhesion activity on its own but exhibits robust homophilic cell adhesion when in a complex with cdh2 and appears to mediate the adhesion while cdh2 acts as a cell adhesion cofactor in the complex (PubMed : 21115806, PubMed : 22184198, PubMed : 33004519). Contributes to neural progenitor cell patterning by promoting homophilic cell interactions (PubMed : 33004519). Required for proper neurite branching. Required for pre- and postsynaptic organization (By similarity).

See full target information cdh2

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Annals of translational medicine 10:79 PubMed35282061

2022

Downregulation of lncRNA NEAT1 inhibits the proliferation of human cutaneous squamous cell carcinoma and .

Applications

Unspecified application

Species

Unspecified reactive species

Zhenhua Gong,Guoliang Shen,Chunhui Huang,Jianchao Zhang,Jianfeng Ji

JCI insight 6: PubMed34784297

2021

Protease-dependent defects in N-cadherin processing drive PMM2-CDG pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Elsenoor J Klaver,Lynn Dukes-Rimsky,Brijesh Kumar,Zhi-Jie Xia,Tammie Dang,Mark A Lehrman,Peggi Angel,Richard R Drake,Hudson H Freeze,Richard Steet,Heather Flanagan-Steet

Development (Cambridge, England) 148: PubMed34131730

2021

Glypican 4 regulates planar cell polarity of endoderm cells by controlling the localization of Cadherin 2.

Applications

Unspecified application

Species

Unspecified reactive species

Anurag Kakkerla Balaraju,Bo Hu,Juan J Rodriguez,Matthew Murry,Fang Lin

International journal of molecular sciences 22: PubMed33671840

2021

Segregates with Microphthalmia and Congenital Cataracts in Two Unrelated Families.

Applications

Unspecified application

Species

Unspecified reactive species

Philippa Harding,Maria Toms,Elena Schiff,Nicholas Owen,Suzannah Bell,Ian Christopher Lloyd,Mariya Moosajee

View all publications

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Product promise

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