AB76057

Anti-N Cadherin antibody

Name: Anti-N Cadherin antibody (ab76057) | Abcam
Brand: Abcam Limited
SKU: AB76057
Price: 620 USD
Availability: InStock
Rating: 5 (20 reviews)

(20 Reviews)

(253 Publications)

Rabbit Polyclonal N Cadherin antibody. Suitable for IP and reacts with Mouse samples. Cited in 253 publications. Immunogen corresponding to Synthetic Peptide within Human CDH2 aa 800-850.

View Alternative Names

CD325, CDHN, NCAD, CDH2, Cadherin-2, CDw325, Neural cadherin, N-cadherin

1 Images

Supplier Data

Immunoprecipitation - Anti-N Cadherin antibody (AB76057)

Western blot image of mouse brain lysate immunoprecipitated with no antibody (lane 1), anti-N-Cadherin (CP1751) rabbit polyclonal antibody (lane 2), and whole mouse brain lysate (lane 3). The blot was probed with anti-N-cadherin (Cytoplasmic) mouse monoclonal antibody (lanes 1-3) and detected using anti-Mouse Ig Light Chain specific : HRP secondary antibody.

All lanes:

Immunoprecipitation - Anti-N Cadherin antibody (ab76057)

Predicted band size: 99 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

applications

Immunogen

Synthetic Peptide within Human CDH2 aa 800-850. The exact immunogen used to generate this antibody is proprietary information.

P19022

Specificity

ab76057 does not crossreact with E Cadherin.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100", "IP-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

N-Cadherin also known as CDH2 or neuronal cadherin is a calcium-dependent cell adhesion protein. It is characterized by its role in mediating intercellular connections primarily through its presence on neurons fibroblasts and certain epithelial cells. N-Cadherin has an approximate mass of 130 kDa. The protein is integral in forming homophilic interactions where N-Cadherin molecules on adjacent cells interact to establish robust cell-cell adhesion.

Biological function summary

N-Cadherin significantly influences cell-cell interaction and communication facilitating cellular adhesion and signal transduction. It is a vital component of adherens junctions contributing to tissue morphogenesis and stability. N-Cadherin interacts with other cytoplasmic proteins such as catenins forming a complex essential for linking the actin cytoskeleton to the cell membrane. This interaction affects cellular behaviors including migration and differentiation.

Pathways

N-Cadherin plays a significant role in the neural development and epithelial-to-mesenchymal transition (EMT) pathways important for development and cancer progression. It engages with related proteins such as beta-catenin which helps transduce signals within these pathways. N-Cadherin's interactions within these pathways highlight its role in maintaining multicellular structure and signaling processes important for development and pathogenesis.

N-Cadherin is associated with cancer and heart disease. In cancer particularly in the context of the EMT N-Cadherin facilitates tumor progression and metastasis cooperating with proteins like Twist and Snail to promote these processes. In heart disease alterations in N-Cadherin expression and function can impact cardiac muscle integrity and syncytium highlighting potential interactions with connexin43 to maintain cardiac function. Understanding these associations provides insights into therapeutic targets for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium-dependent cell adhesion protein; preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone : upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. Plays a role in cell-to-cell junction formation between pancreatic beta cells and neural crest stem (NCS) cells, promoting the formation of processes by NCS cells (By similarity). Required for proper neurite branching. Required for pre- and postsynaptic organization (By similarity). CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.

See full target information CDH2

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Publications (253)

Recent publications for all applications. Explore the full list and refine your search

NPJ Regenerative medicine 10:17 PubMed40175378

2025

Preclinical efficacy and safety of AAVrh10-based plakophilin-2 gene therapy (LX2020) as a treatment for arrhythmogenic cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Zhang,Erika Joana Gutierrez-Lara,Aryanne Do,Lena Nguyen,Anju Nair,Nithya Selvan,Tim Fenn,Eric Adler,Richie Khanna,Farah Sheikh

Pulmonary circulation 14:e70013 PubMed39582775

2024

High gestational leucine level dampens WDPCP/MAPK signaling to impair the EMT and migration of cardiac microvascular endothelial cells in congenital heart defects.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Hong,Guozhou You,Zhongming Luo,Mingxiang Zhang,Jian Chen

Journal of gastroenterology and hepatology 39:2926-2939 PubMed39496400

2024

TYROBP promotes the spread of pancreatic cancer by causing M2 TAM polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Dingwen Zhong,Yonghui Liao,Wenhui Chen,Xianyu Huang,Jiaxin Liu,Zheng Wang

Nature cardiovascular research 2:1246-1261 PubMed39196150

2024

Plakophilin 2 gene therapy prevents and rescues arrhythmogenic right ventricular cardiomyopathy in a mouse model harboring patient genetics.

Applications

Unspecified application

Species

Unspecified reactive species

William H Bradford,Jing Zhang,Erika J Gutierrez-Lara,Yan Liang,Aryanne Do,Tsui-Min Wang,Lena Nguyen,Nirosh Mataraarachchi,Jie Wang,Yusu Gu,Andrew McCulloch,Kirk L Peterson,Farah Sheikh

Journal of biochemical and molecular toxicology 38:e23769 PubMed39152098

2024

Cancer-associated fibroblasts derived exosomal LINC01833 promotes the occurrence of non-small cell lung cancer through miR-335-5p -VAPA axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Chen,Jian-Jun Sun,Ya-Wen Ma,Meng-Qin Zhu,Jing Hu,Qi-Jue Lu,Zhi-Gang Cai

Journal of cellular and molecular medicine 28:e18488 PubMed39031896

2024

MCM8 promotes lung cancer progression through upregulating DNAJC10.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Cao,Hongsheng Liu,Zhijun Han,Cheng Huang,Chao Guo,Luo Zhao,Chao Gao,Yuan Xu,Guige Wang,Zhe Feng,Shanqing Li

Journal of cancer research and clinical oncology 150:335 PubMed38969831

2024

UBQLN4 promotes the proliferation and invasion of non-small cell lung cancer cell by regulating PI3K/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Li He,Heng Chen,Bin Ruan,Li He,Ming Luo,Yulun Fu,Rui Zou

American journal of cancer research 14:1831-1849 PubMed38726274

2024

Construction of gastric cancer prognostic signature based on the E26 transcription factor and the identification of novel oncogene ELK3.

Applications

Unspecified application

Species

Unspecified reactive species

Chenxi Liu,Liqiang Zhou,Zhiqing Chen

Pharmaceutics 16: PubMed38543236

2024

Conjugated Linoleic Acid-Carboxymethyl Chitosan Polymeric Micelles to Improve the Solubility and Oral Bioavailability of Paclitaxel.

Applications

Unspecified application

Species

Unspecified reactive species

Iqra Mubeen,Ghulam Abbas,Shahid Shah,Abdullah A Assiri

Frontiers in oncology 13:1227789 PubMed38033505

2023

Long non-coding RNA PRR7-AS1 promotes osteosarcoma progression via binding RNF2 to transcriptionally suppress MTUS1.

Applications

Unspecified application

Species

Unspecified reactive species

Gu Chen-Xi,Xu Jin-Fu,Huang An-Quan,Yu Xiao,Wu Ying-Hui,Li Suo-Yuan,Shen Cong,Zou Tian-Ming,Shen Jun

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-N Cadherin antibody

Immunoprecipitation - Anti-N Cadherin antibody (AB76057)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (253)

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