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Rabbit Recombinant Monoclonal N Cadherin antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples.


Images

Western blot - Anti-N Cadherin antibody [EPR1791-4] - BSA and Azide free (AB271856), expandable thumbnail
  • Western blot - Anti-N Cadherin antibody [EPR1791-4] - BSA and Azide free (AB271856), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR1791-4] - BSA and Azide free (AB271856), expandable thumbnail
  • Western blot - Anti-N Cadherin antibody [EPR1791-4] - BSA and Azide free (AB271856), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR1791-4] - BSA and Azide free (AB271856), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBIHC-PICC/IF
Human
Not recommended
Tested
Tested
Not recommended
Mouse
Not recommended
Tested
Expected
Not recommended
Rat
Not recommended
Tested
Expected
Not recommended

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Species

Rat

Dilution info

-

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Species

Human

Dilution info

-

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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    6 products for Alternative Version

      Target data

      Function

      Calcium-dependent cell adhesion protein; preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. Plays a role in cell-to-cell junction formation between pancreatic beta cells and neural crest stem (NCS) cells, promoting the formation of processes by NCS cells (By similarity). CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.

      Alternative names

      Cadherin-2, CDw325, Neural cadherin, N-cadherin, NCAD, CDHN, CDH2

      Recommended products

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      Rabbit Recombinant Monoclonal N Cadherin antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples.

      Alternative names

      Cadherin-2, CDw325, Neural cadherin, N-cadherin, NCAD, CDHN, CDH2

      Key facts

      Isotype

      IgG

      Form

      Liquid

      Clonality

      Monoclonal

      Immunogen
      • The exact immunogen used to generate this antibody is proprietary information.
      Carrier free

      Yes

      Clone number

      EPR1791-4

      Purification technique

      Affinity purification Protein A

      Concentration
      Loading...

      Storage

      Shipped at conditions

      Blue Ice

      Appropriate long-term storage conditions

      +4°C

      Storage information

      Do Not Freeze

      Notes

      ab271856 is the carrier-free version of Anti-N Cadherin antibody [EPR1791-4] ab76011.

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free batch production

      For more information, read more on recombinant antibodies.

      Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

      This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

      Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

      This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

      Supplementary info

      Activity summary

      N-Cadherin also known as CDH2 or neuronal cadherin is a calcium-dependent cell adhesion protein. It is characterized by its role in mediating intercellular connections primarily through its presence on neurons fibroblasts and certain epithelial cells. N-Cadherin has an approximate mass of 130 kDa. The protein is integral in forming homophilic interactions where N-Cadherin molecules on adjacent cells interact to establish robust cell-cell adhesion.

      Biological function summary

      N-Cadherin significantly influences cell-cell interaction and communication facilitating cellular adhesion and signal transduction. It is a vital component of adherens junctions contributing to tissue morphogenesis and stability. N-Cadherin interacts with other cytoplasmic proteins such as catenins forming a complex essential for linking the actin cytoskeleton to the cell membrane. This interaction affects cellular behaviors including migration and differentiation.

      Pathways

      N-Cadherin plays a significant role in the neural development and epithelial-to-mesenchymal transition (EMT) pathways important for development and cancer progression. It engages with related proteins such as beta-catenin which helps transduce signals within these pathways. N-Cadherin's interactions within these pathways highlight its role in maintaining multicellular structure and signaling processes important for development and pathogenesis.

      Associated diseases and disorders

      N-Cadherin is associated with cancer and heart disease. In cancer particularly in the context of the EMT N-Cadherin facilitates tumor progression and metastasis cooperating with proteins like Twist and Snail to promote these processes. In heart disease alterations in N-Cadherin expression and function can impact cardiac muscle integrity and syncytium highlighting potential interactions with connexin43 to maintain cardiac function. Understanding these associations provides insights into therapeutic targets for these conditions.

      Product promise

      We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

      In the unlikely event of one of our products not working as expected, you are covered by our product promise.

      Full details and terms and conditions can be found here:
      Terms & Conditions.

      8 product images

      • Western blot - Anti-N Cadherin antibody [EPR1791-4] - BSA and Azide free (ab271856), expandable thumbnail

        Western blot - Anti-N Cadherin antibody [EPR1791-4] - BSA and Azide free (ab271856)

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-N Cadherin antibody [EPR1791-4] ab76011).

        Lanes 1 - 2: Merged signal (red and green). Green - Anti-N Cadherin antibody [EPR1791-4] ab76011 observed at 125 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.

        Anti-N Cadherin antibody [EPR1791-4] ab76011 was shown to react with N Cadherin in wild-type HEK-293T. Loss of signal was observed when knockout cell line Human CDH2 (N Cadherin) knockout HEK-293T cell line ab255377 (knockout cell lysate Human CDH2 (N Cadherin) knockout HEK-293T cell lysate ab263843) was used. Wild-type and N Cadherin knockout samples were subjected to SDS-PAGE. Anti-N Cadherin antibody [EPR1791-4] ab76011 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 5000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

        All lanes: Anti-N Cadherin antibody [EPR1791-4] ab76011, Anti-N Cadherin antibody [EPR1791-4] (Left) or Anti-N Cadherin antibody [EPR19654] ab207608, Anti-N Cadherin antibody [EPR19654] (Right), 1/1000 dilution

        Lane 1: A549 (Human lung carcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method at 20 µg

        Lane 2: PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method at 20 µg

        Lane 3: HCT 116 (Human colorectal carcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method at 20 µg

        Lane 4: HCT 116 (Human colorectal carcinoma epithelial cell) whole cell lysates prepared in 1%SDS Hot lysis method at 20 µg

        Lane 5: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate prepared in RIPA lysis method at 20 µg

        Lane 6: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate prepared in 1%SDS Hot lysis method at 20 µg

        Lane 7: C6 (Rat glial tumor glial cell) whole cell lysates prepared in RIPA lysis method at 20 µg

        Lane 8: C6 (Rat glial tumor glial cell) whole cell lysates prepared in 1%SDS Hot lysis method at 20 µg

        Lane 9: Human brain lysates prepared in 1%SDS Hot lysis method at 20 µg

        Lane 10: Mouse brain lysates prepared in 1%SDS Hot lysis method at 20 µg

        Lane 11: Rat brain lysates prepared in 1%SDS Hot lysis method at 20 µg

        Secondary

        All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

        Predicted band size: 99 kDa

        Observed band size: 110-130 kDa

      • Western blot - Anti-N Cadherin antibody [EPR1791-4] - BSA and Azide free (ab271856), expandable thumbnail

        Western blot - Anti-N Cadherin antibody [EPR1791-4] - BSA and Azide free (ab271856)

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-N Cadherin antibody [EPR1791-4] ab76011).

        Lanes 1 - 2: Merged signal (red and green). Green - Anti-N Cadherin antibody [EPR1791-4] ab76011 observed at 125 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.

        Anti-N Cadherin antibody [EPR1791-4] ab76011 was shown to react with N Cadherin in wild-type HEK-293T. Loss of signal was observed when knockout cell line Human CDH2 (N Cadherin) knockout HEK-293T cell line ab255377 (knockout cell lysate Human CDH2 (N Cadherin) knockout HEK-293T cell lysate ab263843) was used. Wild-type and N Cadherin knockout samples were subjected to SDS-PAGE. Anti-N Cadherin antibody [EPR1791-4] ab76011 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 5000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

        All lanes: Western blot - Anti-N Cadherin antibody [EPR1791-4] - BSA and Azide free (ab271856) at 1/5000 dilution

        Lane 1: Wild-type HEK-293T cell lysate at 20 µg

        Lane 2: CDH2 knockout HEK-293T cell lysate at 20 µg

        Predicted band size: 99 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR1791-4] - BSA and Azide free (ab271856), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR1791-4] - BSA and Azide free (ab271856)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cardiac muscle tissue sections labeling N Cadherin with purified Anti-N Cadherin antibody [EPR1791-4] ab76011 at 1:50 dilution (1.94 µg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-N Cadherin antibody [EPR1791-4] ab76011).

      • Western blot - Anti-N Cadherin antibody [EPR1791-4] - BSA and Azide free (ab271856), expandable thumbnail

        Western blot - Anti-N Cadherin antibody [EPR1791-4] - BSA and Azide free (ab271856)

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-N Cadherin antibody [EPR1791-4] ab76011).

        This blot was produced using a 4-12% Bis-tris under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was blocked for an hour using 3% milk before Anti-N Cadherin antibody [EPR1791-4] ab76011 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at a 1/1000 dilution and 1/20000 dilution respectively. Antibody binding was detected using Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

        All lanes: Western blot - Anti-N Cadherin antibody [EPR1791-4] - BSA and Azide free (ab271856) at 1/1000 dilution

        Lane 1: HeLa Whole Cell Lysate at 20 µg

        Lane 2: HeLa Whole Cell Lysate (Scraped) at 20 µg

        Lane 3: Human Brain Tissue Lysate at 20 µg

        Lane 4: Mouse Brain Tissue Lysate at 20 µg

        Lane 5: Rat Brain Tissue Lysate at 20 µg

        Lane 6: MCF7 Whole Cell Lysate at 20 µg

        Performed under reducing conditions.

        Predicted band size: 99 kDa

        Observed band size: 125 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR1791-4] - BSA and Azide free (ab271856), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR1791-4] - BSA and Azide free (ab271856)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling N Cadherin with purified Anti-N Cadherin antibody [EPR1791-4] ab76011 at 1:50 dilution (1.94 µg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-N Cadherin antibody [EPR1791-4] ab76011).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR1791-4] - BSA and Azide free (ab271856), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR1791-4] - BSA and Azide free (ab271856)

        Immunohistochemistry of kidney carcinoma staining N Cadherin with Anti-N Cadherin antibody [EPR1791-4] ab76011 at 1μg/ml

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-N Cadherin antibody [EPR1791-4] ab76011).

      • Western blot - Anti-N Cadherin antibody [EPR1791-4] - BSA and Azide free (ab271856), expandable thumbnail

        Western blot - Anti-N Cadherin antibody [EPR1791-4] - BSA and Azide free (ab271856)

        This data was developed using the same antibody clone in a different buffer formulation (Anti-N Cadherin antibody [EPR1791-4] ab76011).

        False colour image of Western blot: Anti-N Cadherin antibody [EPR1791-4] staining at 1/5000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-N Cadherin antibody [EPR1791-4] ab76011 was shown to bind specifically to N Cadherin. A band was observed at 125 kDa in wild-type HeLa cell lysates with no signal observed at this size in cdh2 knockout cell line ab274934 (knockout cell lysate ab274992).

        To generate this image, wild-type and cdh2 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

        Lanes 1 - 2: Western blot - Anti-N Cadherin antibody [EPR1791-4] (Anti-N Cadherin antibody [EPR1791-4] ab76011) at 1/5000 dilution

        Lanes 1 - 2: Western blot - Anti-N Cadherin antibody [EPR1791-4] - BSA and Azide free (ab271856)

        Lanes 1 - 2: Western blot - Anti-N Cadherin antibody [EPR1791-4] - Low endotoxin, Azide free (Anti-N Cadherin antibody [EPR1791-4] - Low endotoxin, Azide free ab202030) at 1/5000 dilution

        Lane 1: Wild-type HeLa cell lysate at 20 µg

        Lane 2: cdh2 knockout HeLa cell lysate at 20 µg

        Secondary

        All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

        Performed under reducing conditions.

        Observed band size: 125 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR1791-4] - BSA and Azide free (ab271856), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR1791-4] - BSA and Azide free (ab271856)

        Immunohistochemical analysis of formalin-fixed paraffin-embedded human heart labelling N Cadherin with ab271856 at a concentration of 1µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32min with ULTRA cell conditioning solution (CC1 pH8.5). ab271856 anti N Cadherin antibody was incubated at 37°C for 16min. Sections were counterstained is with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

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      Product protocols

      For this product, it's our understanding that no specific protocols are required. You can:

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