Knockout Tested Rabbit Recombinant Monoclonal N Cadherin antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 52 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR1791-4] - Low endotoxin, Azide free (AB202030), expandable thumbnail](https://content.abcam.com/products/images/n-cadherin-antibody-epr1791-4-low-endotoxin-azide-free-ab202030--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137136.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR1791-4] - Low endotoxin, Azide free (AB202030)")
![Western blot - Anti-N Cadherin antibody [EPR1791-4] - Low endotoxin, Azide free (AB202030), expandable thumbnail](https://content.abcam.com/products/images/n-cadherin-antibody-epr1791-4-low-endotoxin-azide-free-ab202030--western-blot-img55322.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-N Cadherin antibody [EPR1791-4] - Low endotoxin, Azide free (AB202030)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR1791-4] - Low endotoxin, Azide free (AB202030), expandable thumbnail](https://content.abcam.com/products/images/n-cadherin-antibody-epr1791-4-low-endotoxin-azide-free-ab202030--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107979.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR1791-4] - Low endotoxin, Azide free (AB202030)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR1791-4] - Low endotoxin, Azide free (AB202030), expandable thumbnail](https://content.abcam.com/products/images/n-cadherin-antibody-epr1791-4-low-endotoxin-azide-free-ab202030--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104049.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR1791-4] - Low endotoxin, Azide free (AB202030)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR1791-4] - Low endotoxin, Azide free (AB202030), expandable thumbnail](https://content.abcam.com/products/images/n-cadherin-antibody-epr1791-4-low-endotoxin-azide-free-ab202030--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img102345.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR1791-4] - Low endotoxin, Azide free (AB202030)")
Publications
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- Journal of gastrointestinal oncology 13:1444-14532022UBE2C promotes the progression of pancreatic cancer and glycolytic activity via EGFR stabilization-mediated PI3K-Akt pathway activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing-Zhu Cao,Gang Nie,Hao Hu,Xiao Zhang,Chen-Ming Ni,Zhi-Ping Huang,Guang-Lei Qiao,Liu OuyangPubMed 35837197
- Annals of translational medicine 9:17222022Aloe-emodin inhibits the proliferation, migration, and invasion of melanoma cells via inactivation of the Wnt/beta-catenin signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaotao Du,Pan Shen,Ranjing Tan,Dengyan Wu,Shenghao TuPubMed 35071416
- Cell death & disease 12:10472021Linc-ROR facilitates progression and angiogenesis of hepatocellular carcinoma by modulating DEPDC1 expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChuan Tian,Mubalake Abudoureyimu,Xinrong Lin,Xiaoyuan Chu,Rui WangPubMed 34741030
- Cancer cell international 21:4292021LINC01410 leads the migration, invasion and EMT of bladder cancer cells by modulating miR-4319 / Snail1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Guo,Qimei Gai,Yue Ma,Zhengfei Shan,Jitao WuPubMed 34391433
- American journal of translational research 13:7524-75372021LINC00671 inhibits renal cell cancer progression via regulating miR-221-5p/SOCS1 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGang Jin,Haiyan Mi,Yunfei Ye,Qi Yao,Lei Yuan,Xiaoxiong WuPubMed 34377233
- Oncology reports 46:2021Long non‑coding RNA PCED1B‑AS1 promotes pancreatic ductal adenocarcinoma progression by regulating the miR‑411‑3p/HIF‑1α axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi Zhang,Huan Ma,Chang ChenPubMed 34036383
- Open medicine (Warsaw, Poland) 16:805-8152021Functional implications of PABPC1 in the development of ovarian cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCong Feng,Yan-Hua Han,Na Qi,Jia Li,Qing-Hua Sheng,Yu Liu,Li-Li YangPubMed 34027108
- Experimental and therapeutic medicine 22:6982021High expression of spermatogenesis associated serine rich 2 promotes tumorigenicity in esophageal squamous cell carcinoma cells and is associated with poor patient prognosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan-Peng Liu,Qiuhong Cao,Ling Li,Min ZhangPubMed 33986862
- Leukemia & lymphoma 62:1574-15842021lncRNA SNHG14 promotes oncogenesis and immune evasion in diffuse large-B-cell lymphoma by sequestering miR-152-3p.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuyang Tian,Lianqiao Li,Guoqiang Lin,Yan Wang,Li Wang,Qian Zhao,Youdong Hu,Hongmei Yong,Yan Wan,Yanming ZhangPubMed 33682607
- OncoTargets and therapy 14:393-4022021Long Non-Coding RNA PCED1B-AS1 Promotes the Progression of Clear Cell Renal Cell Carcinoma Through miR-484/ZEB1 Axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianhua Qin et. al.PubMed 33469315
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Not recommended | Tested | Tested | Not recommended |
| Mouse | Not recommended | Tested | Expected | Not recommended |
| Rat | Not recommended | Tested | Expected | Not recommended |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Mouse | Dilution info - | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Rat | Dilution info - | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
4 products for Alternative Version
Target data
Function
Calcium-dependent cell adhesion protein; preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. Plays a role in cell-to-cell junction formation between pancreatic beta cells and neural crest stem (NCS) cells, promoting the formation of processes by NCS cells (By similarity). Required for proper neurite branching. Required for pre- and postsynaptic organization (By similarity). CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.
Alternative names
CDHN, NCAD, NCAD, CDHN, CDH2, Cadherin-2, CDw325, Neural cadherin, N-cadherin
Recommended products
Knockout Tested Rabbit Recombinant Monoclonal N Cadherin antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 52 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR1791-4
- Purification technique
Affinity purification Protein A
- Specificity
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab202030 is the carrier-free version of Anti-N Cadherin antibody [EPR1791-4] ab76011.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Our Low endotoxin, azide-free formats have low endotoxin level (1 EU/mg, determined by the TAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
N-Cadherin also known as CDH2 or neuronal cadherin is a calcium-dependent cell adhesion protein. It is characterized by its role in mediating intercellular connections primarily through its presence on neurons fibroblasts and certain epithelial cells. N-Cadherin has an approximate mass of 130 kDa. The protein is integral in forming homophilic interactions where N-Cadherin molecules on adjacent cells interact to establish robust cell-cell adhesion.
- Biological function summary
N-Cadherin significantly influences cell-cell interaction and communication facilitating cellular adhesion and signal transduction. It is a vital component of adherens junctions contributing to tissue morphogenesis and stability. N-Cadherin interacts with other cytoplasmic proteins such as catenins forming a complex essential for linking the actin cytoskeleton to the cell membrane. This interaction affects cellular behaviors including migration and differentiation.
- Pathways
N-Cadherin plays a significant role in the neural development and epithelial-to-mesenchymal transition (EMT) pathways important for development and cancer progression. It engages with related proteins such as beta-catenin which helps transduce signals within these pathways. N-Cadherin's interactions within these pathways highlight its role in maintaining multicellular structure and signaling processes important for development and pathogenesis.
- Associated diseases and disorders
N-Cadherin is associated with cancer and heart disease. In cancer particularly in the context of the EMT N-Cadherin facilitates tumor progression and metastasis cooperating with proteins like Twist and Snail to promote these processes. In heart disease alterations in N-Cadherin expression and function can impact cardiac muscle integrity and syncytium highlighting potential interactions with connexin43 to maintain cardiac function. Understanding these associations provides insights into therapeutic targets for these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR1791-4] - Low endotoxin, Azide free (ab202030), expandable thumbnail](https://content.abcam.com/products/images/n-cadherin-antibody-epr1791-4-low-endotoxin-azide-free-ab202030--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137136.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR1791-4] - Low endotoxin, Azide free (ab202030)")
This image is courtesy of an anonymous customer review.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR1791-4] - Low endotoxin, Azide free (ab202030)
Anti-N Cadherin antibody [EPR1791-4] ab76011 staining N Cadherin in Mouse pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA + 1% FBS for 2 hours at room temperature; antigen retrieval was by heat mediation in a citrate buffer pH6. Samples were incubated with primary antibody (1/500 in 1% BSA + 1% FBS) for 16 hours at 4°C. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-N Cadherin antibody [EPR1791-4] ab76011).
![Western blot - Anti-N Cadherin antibody [EPR1791-4] - Low endotoxin, Azide free (ab202030), expandable thumbnail](https://content.abcam.com/products/images/n-cadherin-antibody-epr1791-4-low-endotoxin-azide-free-ab202030--western-blot-img55322.jpg "Western blot - Anti-N Cadherin antibody [EPR1791-4] - Low endotoxin, Azide free (ab202030)")
Western blot - Anti-N Cadherin antibody [EPR1791-4] - Low endotoxin, Azide free (ab202030)
This data was developed using the same antibody clone in a different buffer formulation (Anti-N Cadherin antibody [EPR1791-4] ab76011).
Lanes 1 - 2: Merged signal (red and green). Green - Anti-N Cadherin antibody [EPR1791-4] ab76011 observed at 125 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.
Anti-N Cadherin antibody [EPR1791-4] ab76011 was shown to react with N Cadherin in wild-type HEK-293T. Loss of signal was observed when knockout cell line Human CDH2 (N Cadherin) knockout HEK-293T cell line ab255377 (knockout cell lysate Human CDH2 (N Cadherin) knockout HEK-293T cell lysate ab263843) was used. Wild-type and N Cadherin knockout samples were subjected to SDS-PAGE. Anti-N Cadherin antibody [EPR1791-4] ab76011 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 5000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-N Cadherin antibody [EPR1791-4] (Anti-N Cadherin antibody [EPR1791-4] ab76011) at 1/5000 dilution
Lane 1: Wild-type HEK-293T cell lysate at 20 µg
Lane 2: CDH2 knockout HEK-293T cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 99 kDa
Observed band size: 100 kDa, 125 kDa
Lanes 1 - 2: Merged signal (red and green). Green - Anti-N Cadherin antibody [EPR1791-4] ab76011 observed at 125 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.
Anti-N Cadherin antibody [EPR1791-4] ab76011 was shown to react with N Cadherin in wild-type HEK-293T. Loss of signal was observed when knockout cell line Human CDH2 (N Cadherin) knockout HEK-293T cell line ab255377 (knockout cell lysate Human CDH2 (N Cadherin) knockout HEK-293T cell lysate ab263843) was used. Wild-type and N Cadherin knockout samples were subjected to SDS-PAGE. Anti-N Cadherin antibody [EPR1791-4] ab76011 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 5000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR1791-4] - Low endotoxin, Azide free (ab202030), expandable thumbnail](https://content.abcam.com/products/images/n-cadherin-antibody-epr1791-4-low-endotoxin-azide-free-ab202030--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107979.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR1791-4] - Low endotoxin, Azide free (ab202030)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR1791-4] - Low endotoxin, Azide free (ab202030)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cardiac muscle tissue sections labeling N Cadherin with purified Anti-N Cadherin antibody [EPR1791-4] ab76011 at 1:50 dilution (1.94 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR1791-4] - Low endotoxin, Azide free (ab202030), expandable thumbnail](https://content.abcam.com/products/images/n-cadherin-antibody-epr1791-4-low-endotoxin-azide-free-ab202030--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104049.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR1791-4] - Low endotoxin, Azide free (ab202030)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR1791-4] - Low endotoxin, Azide free (ab202030)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling N Cadherin with purified Anti-N Cadherin antibody [EPR1791-4] ab76011 at 1:50 dilution (1.94 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR1791-4] - Low endotoxin, Azide free (ab202030), expandable thumbnail](https://content.abcam.com/products/images/n-cadherin-antibody-epr1791-4-low-endotoxin-azide-free-ab202030--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img102345.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR1791-4] - Low endotoxin, Azide free (ab202030)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR1791-4] - Low endotoxin, Azide free (ab202030)
Immunohistochemistry of kidney carcinoma staining N Cadherin with Anti-N Cadherin antibody [EPR1791-4] ab76011 at 1μg/ml
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-N Cadherin antibody [EPR1791-4] ab76011).
![Western blot - Anti-N Cadherin antibody [EPR1791-4] - Low endotoxin, Azide free (ab202030), expandable thumbnail](https://content.abcam.com/products/images/n-cadherin-antibody-epr1791-4-low-endotoxin-azide-free-ab202030--western-blot-img418049.jpg "Western blot - Anti-N Cadherin antibody [EPR1791-4] - Low endotoxin, Azide free (ab202030)")
Western blot - Anti-N Cadherin antibody [EPR1791-4] - Low endotoxin, Azide free (ab202030)
This data was developed using the same antibody clone in a different buffer formulation (Anti-N Cadherin antibody [EPR1791-4] ab76011).
False colour image of Western blot: Anti-N Cadherin antibody [EPR1791-4] staining at 1/5000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-N Cadherin antibody [EPR1791-4] ab76011 was shown to bind specifically to N Cadherin. A band was observed at 125 kDa in wild-type HeLa cell lysates with no signal observed at this size in cdh2 knockout cell line ab274934 (knockout cell lysate ab274992).
To generate this image, wild-type and cdh2 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilutionLanes 1 - 2: Western blot - Anti-N Cadherin antibody [EPR1791-4] (Anti-N Cadherin antibody [EPR1791-4] ab76011) at 1/5000 dilution
Lanes 1 - 2: Western blot - Anti-N Cadherin antibody [EPR1791-4] - BSA and Azide free (Anti-N Cadherin antibody [EPR1791-4] - BSA and Azide free ab271856)
Lanes 1 - 2: Western blot - Anti-N Cadherin antibody [EPR1791-4] - Low endotoxin, Azide free (ab202030) at 1/5000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: cdh2 knockout HeLa cell lysate at 20 µg
SecondaryAll lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 125 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR1791-4] - Low endotoxin, Azide free (ab202030), expandable thumbnail](https://content.abcam.com/products/images/n-cadherin-antibody-epr1791-4-low-endotoxin-azide-free-ab202030--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img426002.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR1791-4] - Low endotoxin, Azide free (ab202030)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR1791-4] - Low endotoxin, Azide free (ab202030)
Immunohistochemical analysis of formalin-fixed paraffin-embedded human heart labelling N Cadherin with Anti-N Cadherin antibody [EPR1791-4] - BSA and Azide free ab271856 at a concentration of 1µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32min with ULTRA cell conditioning solution (CC1 pH8.5). Anti-N Cadherin antibody [EPR1791-4] - BSA and Azide free ab271856 anti N Cadherin antibody was incubated at 37°C for 16min. Sections were counterstained is with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation (Anti-N Cadherin antibody [EPR1791-4] - BSA and Azide free ab271856).
![Western blot - Anti-N Cadherin antibody [EPR1791-4] - Low endotoxin, Azide free (ab202030), expandable thumbnail](https://content.abcam.com/products/images/n-cadherin-antibody-epr1791-4-low-endotoxin-azide-free-ab202030--western-blot-img418049.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-N Cadherin antibody [EPR1791-4] - Low endotoxin, Azide free (ab202030)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR1791-4] - Low endotoxin, Azide free (ab202030), expandable thumbnail](https://content.abcam.com/products/images/n-cadherin-antibody-epr1791-4-low-endotoxin-azide-free-ab202030--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img426002.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [EPR1791-4] - Low endotoxin, Azide free (ab202030)")
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