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Rabbit Recombinant Monoclonal N Cadherin antibody. Suitable for IP, Flow Cyt, WB and reacts with Human, Mouse, Rat samples. Cited in 23 publications.

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Images

Flow Cytometry - Anti-N Cadherin antibody [EPR22397-264] (AB245117), expandable thumbnail
  • Western blot - Anti-N Cadherin antibody [EPR22397-264] (AB245117), expandable thumbnail
  • Immunoprecipitation - Anti-N Cadherin antibody [EPR22397-264] (AB245117), expandable thumbnail
  • Western blot - Anti-N Cadherin antibody [EPR22397-264] (AB245117), expandable thumbnail
  • Western blot - Anti-N Cadherin antibody [EPR22397-264] (AB245117), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-PICC/IF
Human
Tested
Tested
Tested
Not recommended
Not recommended
Mouse
Expected
Expected
Tested
Not recommended
Not recommended
Rat
Expected
Expected
Tested
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

1/30

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Species

Human

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

Calcium-dependent cell adhesion protein; preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. Plays a role in cell-to-cell junction formation between pancreatic beta cells and neural crest stem (NCS) cells, promoting the formation of processes by NCS cells (By similarity). Required for proper neurite branching. Required for pre- and postsynaptic organization (By similarity). CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.

Alternative names

CDHN, NCAD, NCAD, CDHN, CDH2, Cadherin-2, CDw325, Neural cadherin, N-cadherin

Recommended products

Rabbit Recombinant Monoclonal N Cadherin antibody. Suitable for IP, Flow Cyt, WB and reacts with Human, Mouse, Rat samples. Cited in 23 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR22397-264

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

N-Cadherin also known as CDH2 or neuronal cadherin is a calcium-dependent cell adhesion protein. It is characterized by its role in mediating intercellular connections primarily through its presence on neurons fibroblasts and certain epithelial cells. N-Cadherin has an approximate mass of 130 kDa. The protein is integral in forming homophilic interactions where N-Cadherin molecules on adjacent cells interact to establish robust cell-cell adhesion.

Biological function summary

N-Cadherin significantly influences cell-cell interaction and communication facilitating cellular adhesion and signal transduction. It is a vital component of adherens junctions contributing to tissue morphogenesis and stability. N-Cadherin interacts with other cytoplasmic proteins such as catenins forming a complex essential for linking the actin cytoskeleton to the cell membrane. This interaction affects cellular behaviors including migration and differentiation.

Pathways

N-Cadherin plays a significant role in the neural development and epithelial-to-mesenchymal transition (EMT) pathways important for development and cancer progression. It engages with related proteins such as beta-catenin which helps transduce signals within these pathways. N-Cadherin's interactions within these pathways highlight its role in maintaining multicellular structure and signaling processes important for development and pathogenesis.

Associated diseases and disorders

N-Cadherin is associated with cancer and heart disease. In cancer particularly in the context of the EMT N-Cadherin facilitates tumor progression and metastasis cooperating with proteins like Twist and Snail to promote these processes. In heart disease alterations in N-Cadherin expression and function can impact cardiac muscle integrity and syncytium highlighting potential interactions with connexin43 to maintain cardiac function. Understanding these associations provides insights into therapeutic targets for these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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6 product images

  • Flow Cytometry - Anti-N Cadherin antibody [EPR22397-264] (ab245117), expandable thumbnail

    Flow Cytometry - Anti-N Cadherin antibody [EPR22397-264] (ab245117)

    Flow cytometric analysis of MCF7 (human breast adenocarcinoma epithelial cell line, Left) / HeLa (human cervix adenocarcinoma epithelial cell line, Right) cell lines labeling N Cadherin with ab245117 at 1/500 (red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.

    Negative control: MCF7 (PMID: 9177902).

    Gated on viable cells.

  • Western blot - Anti-N Cadherin antibody [EPR22397-264] (ab245117), expandable thumbnail

    Western blot - Anti-N Cadherin antibody [EPR22397-264] (ab245117)

    Lanes 1-2: Merged signal (red and green). Green - ab245117 observed at 110, 130 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.

    ab245117 was shown to react with N Cadherin in wild-type HEK-293T cells in Western blot. Loss of signal was observed when knockout sample Human CDH2 (N Cadherin) knockout HEK-293T cell lysate ab263843 was used. Wild-type and N Cadherin knockout samples were subjected to SDS-PAGE. ab245117 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 100000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-N Cadherin antibody [EPR22397-264] (ab245117) at 1/1000 dilution

    Lane 1: Wild-type HEK-293T cell lysate at 20 µg

    Lane 2: CDH2 knockout HEK-293T cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 99 kDa

    Observed band size: 125 kDa, 130 kDa

    Lanes 1 - 2: Merged signal (red and green). Green - ab245117 observed at 125 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.

    ab245117 was shown to react with N Cadherin in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line Human CDH2 (N Cadherin) knockout HEK-293T cell line ab255377 (knockout cell lysate Human CDH2 (N Cadherin) knockout HEK-293T cell lysate ab263843) was used. Wild-type and N Cadherin knockout samples were subjected to SDS-PAGE. ab245117 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunoprecipitation - Anti-N Cadherin antibody [EPR22397-264] (ab245117), expandable thumbnail

    Immunoprecipitation - Anti-N Cadherin antibody [EPR22397-264] (ab245117)

    N Cadherin was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell line) whole cell lysate with ab245117 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab245117 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1000 dilution.

    Lane 1: HeLa whole cell lysate 10 μg (Input).
    Lane 2: ab245117 IP in HeLa whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab245117 in HeLa whole cell lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 3 seconds.

    The molecular weight is consistent with literature (PMID: 8230319).

    All lanes: Immunoprecipitation - Anti-N Cadherin antibody [EPR22397-264] (ab245117)

    Predicted band size: 99 kDa

    Observed band size: 110 kDa, 130 kDa

  • Western blot - Anti-N Cadherin antibody [EPR22397-264] (ab245117), expandable thumbnail

    Western blot - Anti-N Cadherin antibody [EPR22397-264] (ab245117)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-3: 26 seconds; Lanes 4-6: 3 minutes; Lane 7: 48 seconds.

    The molecular weight is consistent with literature (PMID: 8230319).

    Negative control: MCF7 (PMID: 9177902).

    All lanes: Western blot - Anti-N Cadherin antibody [EPR22397-264] (ab245117) at 1/1000 dilution

    Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Lane 2: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

    Lane 3: MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg

    Lane 4: PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 5: C6 (rat glial tumor glial cell) whole cell lysate at 10 µg

    Lane 6: A549 (human lung carcinoma cell line) whole cell lysate at 20 µg

    Lane 7: Human brain lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 99 kDa

    Observed band size: 110 kDa, 130 kDa

  • Western blot - Anti-N Cadherin antibody [EPR22397-264] (ab245117), expandable thumbnail

    Western blot - Anti-N Cadherin antibody [EPR22397-264] (ab245117)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 3 minutes; Lane 2: 26 seconds; Lane 3: 48 seconds; Lane 4: 10 seconds; Lane 5: 3 minutes.

    The molecular weight is consistent with literature (PMID: 8230319).

    All lanes: Western blot - Anti-N Cadherin antibody [EPR22397-264] (ab245117) at 1/1000 dilution

    Lane 1: Mouse brain lysate at 10 µg

    Lane 2: Mouse heart lysate at 10 µg

    Lane 3: Rat brain lysate at 10 µg

    Lane 4: Rat heart lysate at 10 µg

    Lane 5: Rat liver lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 99 kDa

    Observed band size: 110 kDa, 130 kDa

  • Western blot - Anti-N Cadherin antibody [EPR22397-264] (ab245117), expandable thumbnail

    Western blot - Anti-N Cadherin antibody [EPR22397-264] (ab245117)

    False colour image of Western blot: Anti-N Cadherin antibody [EPR22397-264] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab245117 was shown to bind specifically to N Cadherin. A band was observed at 125 kDa in wild-type HeLa cell lysates with no signal observed at this size in cdh2 knockout cell line ab274934 (knockout cell lysate ab274992). To generate this image, wild-type and cdh2 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    Lanes 1 - 2: Western blot - Anti-N Cadherin antibody [EPR22397-264] (ab245117) at 1/1000 dilution

    Lanes 1 - 2: Western blot - Anti-N Cadherin antibody [EPR22397-264] - BSA and Azide free (Anti-N Cadherin antibody [EPR22397-264] - BSA and Azide free ab245827) at 1/1000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: cdh2 knockout HeLa cell lysate at 20 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 125 kDa

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