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Knockout Tested Rabbit Recombinant Monoclonal N Cadherin antibody. Suitable for I-ELISA, ICC/IF, WB and reacts with Recombinant fragment - Human, Human, Mouse samples.

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Images

Western blot - Anti-N Cadherin antibody [EPR29626-10] (AB321897), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-N Cadherin antibody [EPR29626-10] (AB321897), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-N Cadherin antibody [EPR29626-10] (AB321897), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-N Cadherin antibody [EPR29626-10] (AB321897), expandable thumbnail
  • Indirect ELISA - Anti-N Cadherin antibody [EPR29626-10] (AB321897), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
I-ELISAICC/IFWB
Human
Expected
Tested
Tested
Mouse
Expected
Tested
Tested
Recombinant fragment - Human
Tested
Not recommended
Not recommended

Tested
Tested

Species

Recombinant fragment - Human

Dilution info

1000 ng/mL

Notes

-

Expected
Expected

Species

Human, Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50

Notes

-

Species

Mouse

Dilution info

1/50

Notes

-

Not recommended
Not recommended

Species

Recombinant fragment - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Recombinant fragment - Human

Dilution info

-

Notes

-

Target data

Function

Calcium-dependent cell adhesion protein; preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. Plays a role in cell-to-cell junction formation between pancreatic beta cells and neural crest stem (NCS) cells, promoting the formation of processes by NCS cells (By similarity). Required for proper neurite branching. Required for pre- and postsynaptic organization (By similarity). CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.

Alternative names

Recommended products

Knockout Tested Rabbit Recombinant Monoclonal N Cadherin antibody. Suitable for I-ELISA, ICC/IF, WB and reacts with Recombinant fragment - Human, Human, Mouse samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR29626-10

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

N-Cadherin also known as CDH2 or neuronal cadherin is a calcium-dependent cell adhesion protein. It is characterized by its role in mediating intercellular connections primarily through its presence on neurons fibroblasts and certain epithelial cells. N-Cadherin has an approximate mass of 130 kDa. The protein is integral in forming homophilic interactions where N-Cadherin molecules on adjacent cells interact to establish robust cell-cell adhesion.

Biological function summary

N-Cadherin significantly influences cell-cell interaction and communication facilitating cellular adhesion and signal transduction. It is a vital component of adherens junctions contributing to tissue morphogenesis and stability. N-Cadherin interacts with other cytoplasmic proteins such as catenins forming a complex essential for linking the actin cytoskeleton to the cell membrane. This interaction affects cellular behaviors including migration and differentiation.

Pathways

N-Cadherin plays a significant role in the neural development and epithelial-to-mesenchymal transition (EMT) pathways important for development and cancer progression. It engages with related proteins such as beta-catenin which helps transduce signals within these pathways. N-Cadherin's interactions within these pathways highlight its role in maintaining multicellular structure and signaling processes important for development and pathogenesis.

Associated diseases and disorders

N-Cadherin is associated with cancer and heart disease. In cancer particularly in the context of the EMT N-Cadherin facilitates tumor progression and metastasis cooperating with proteins like Twist and Snail to promote these processes. In heart disease alterations in N-Cadherin expression and function can impact cardiac muscle integrity and syncytium highlighting potential interactions with connexin43 to maintain cardiac function. Understanding these associations provides insights into therapeutic targets for these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-N Cadherin antibody [EPR29626-10] (ab321897), expandable thumbnail

    Western blot - Anti-N Cadherin antibody [EPR29626-10] (ab321897)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: MCF7 (PMID: 9177902).

    Low expression: 4T1.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 22553038).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-N Cadherin antibody [EPR29626-10] (ab321897) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 2: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: C2C12 (mouse myoblast) whole cell lysate at 20 µg

    Lane 4: 4T1 (mouse mammary gland carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 5: A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 6: Human cerebellum tissue lysate at 20 µg

    Lane 7: Mouse cerebellum tissue lysate at 20 µg

    Lane 8: Mouse brain tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 80-130 kDa, 36 kDa

    Exposure time: 6s

  • Immunocytochemistry/ Immunofluorescence - Anti-N Cadherin antibody [EPR29626-10] (ab321897), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-N Cadherin antibody [EPR29626-10] (ab321897)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized A549 (human lung carcinoma epithelial cell) cells labelling N Cadherin with ab321897 at 1/50 (9.82 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

    Confocal image showing membranous and weak cytoplasmic staining in A549 cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).

    Negative control: MCF7(PMID: 9177902).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-N Cadherin antibody [EPR29626-10] (ab321897), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-N Cadherin antibody [EPR29626-10] (ab321897)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized CDH2 KO HEK-293T (CDH2 knockout human embryonic kidney epithelial cell), Human CDH2 (N Cadherin) knockout HEK-293T cell line ab255377 cells labelling N Cadherin with ab321897 at 1/50 (9.82 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

    Confocal image showing membranous staining in wildtype HEK-293T cells, showing no staining in CDH2 knockout HEK-293T cells(shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-N Cadherin antibody [EPR29626-10] (ab321897), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-N Cadherin antibody [EPR29626-10] (ab321897)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized C2C12 (mouse myoblast) cells labelling N Cadherin with ab321897 at 1/50 (9.82 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

    Confocal image showing membranous and weak cytoplasmic staining in C2C12 cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).

    Low expression: 4T1.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Indirect ELISA - Anti-N Cadherin antibody [EPR29626-10] (ab321897), expandable thumbnail

    Indirect ELISA - Anti-N Cadherin antibody [EPR29626-10] (ab321897)

    Indirect ELISA analysis of ab321897 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution.

    Antigen: Human Cadherin-2,Human Cadherin-4.

    Antigen concentration: 1000 ng/ml

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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