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Rabbit Recombinant Monoclonal N Cadherin antibody. C-terminal. Suitable for Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (AB225719), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (AB225719), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (AB225719), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (AB225719), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-N Cadherin antibody [SP90] - C-terminal (AB225719), expandable thumbnail

Publications

  • Cancers 13:2021
    Impact of Epithelial-Mesenchymal Transition on the Immune Landscape in Breast Cancer.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Fatima-Zohra Khadri,Marianne Samir Makboul Issac,Louis Arthur Gaboury
    PubMed 34680248

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)IHC-P
Human
Tested
Tested
Mouse
Expected
Tested
Rat
Expected
Tested
Cow
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/100

Notes

Antigen retrieval: Boil tissue section in 1mM EDTA, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

Species

Rat

Dilution info

1/100

Notes

Antigen retrieval: Boil tissue section in 1mM EDTA, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

Species

Human

Dilution info

1/100

Notes

Antigen retrieval: Boil tissue section in 1mM EDTA, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

Predicted
Predicted

Species

Cow

Dilution info

-

Notes

-

Associated Products

Select an associated product type

9 products for Alternative Product

    1 products for Alternative Version

      Target data

      Function

      Calcium-dependent cell adhesion protein; preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. Plays a role in cell-to-cell junction formation between pancreatic beta cells and neural crest stem (NCS) cells, promoting the formation of processes by NCS cells (By similarity). CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.

      Alternative names

      Cadherin-2, CDw325, Neural cadherin, N-cadherin, NCAD, CDHN, CDH2

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      Rabbit Recombinant Monoclonal N Cadherin antibody. C-terminal. Suitable for Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

      Alternative names

      Cadherin-2, CDw325, Neural cadherin, N-cadherin, NCAD, CDHN, CDH2

      Key facts

      Isotype

      IgG

      Form

      Liquid

      Clonality

      Monoclonal

      Immunogen
      • The exact immunogen used to generate this antibody is proprietary information.
      Clone number

      SP90

      Purification technique

      Affinity purification Protein A/G

      Concentration
      Loading...
      Purification notes

      Purified from TCS by protein A/G.

      Storage

      Shipped at conditions

      Blue Ice

      Appropriate short-term storage duration

      1-2 weeks

      Appropriate short-term storage conditions

      +4°C

      Appropriate long-term storage conditions

      -20°C

      Aliquoting information

      Upon delivery aliquot

      Storage information

      Avoid freeze / thaw cycle

      Notes

      This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

      Supplementary info

      Activity summary

      N-Cadherin also known as CDH2 or neuronal cadherin is a calcium-dependent cell adhesion protein. It is characterized by its role in mediating intercellular connections primarily through its presence on neurons fibroblasts and certain epithelial cells. N-Cadherin has an approximate mass of 130 kDa. The protein is integral in forming homophilic interactions where N-Cadherin molecules on adjacent cells interact to establish robust cell-cell adhesion.

      Biological function summary

      N-Cadherin significantly influences cell-cell interaction and communication facilitating cellular adhesion and signal transduction. It is a vital component of adherens junctions contributing to tissue morphogenesis and stability. N-Cadherin interacts with other cytoplasmic proteins such as catenins forming a complex essential for linking the actin cytoskeleton to the cell membrane. This interaction affects cellular behaviors including migration and differentiation.

      Pathways

      N-Cadherin plays a significant role in the neural development and epithelial-to-mesenchymal transition (EMT) pathways important for development and cancer progression. It engages with related proteins such as beta-catenin which helps transduce signals within these pathways. N-Cadherin's interactions within these pathways highlight its role in maintaining multicellular structure and signaling processes important for development and pathogenesis.

      Associated diseases and disorders

      N-Cadherin is associated with cancer and heart disease. In cancer particularly in the context of the EMT N-Cadherin facilitates tumor progression and metastasis cooperating with proteins like Twist and Snail to promote these processes. In heart disease alterations in N-Cadherin expression and function can impact cardiac muscle integrity and syncytium highlighting potential interactions with connexin43 to maintain cardiac function. Understanding these associations provides insights into therapeutic targets for these conditions.

      Product promise

      We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

      In the unlikely event of one of our products not working as expected, you are covered by our product promise.

      Full details and terms and conditions can be found here:
      Terms & Conditions.

      13 product images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat liver tissue sections labeling N Cadherin with Purified ab225719 at 1/100 dilution (0.56 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse liver tissue sections labeling N Cadherin with Purified ab225719 at 1/100 dilution (0.56 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human heart tissue sections labeling N Cadherin with Purified ab225719 at 1/100 dilution (0.56 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling N Cadherin with Purified ab225719 at 1/100 dilution (0.56 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

      • Flow Cytometry (Intracellular) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719), expandable thumbnail

        Flow Cytometry (Intracellular) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)

        Flow cytometry analysis of Hela (human cervix adenocarcinoma) labeling N Cadherin with purified ab225719 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black). Unlableled control - Unlabelled cells (blue).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)

        Formalin-fixed, paraffin-embedded human mesothelioma tissue stained for N Cadherin with ab225719 at 1/100 dilution in immunohistochemical analysis.

      • Flow Cytometry (Intracellular) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719), expandable thumbnail

        Flow Cytometry (Intracellular) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)

        Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling N Cadherin with ab225719 (green) compared with an isotype control rabbit IgG (blue).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)

        Formalin-fixed, paraffin-embedded human mesothelioma tissue stained for N Cadherin with ab225719 at 1/100 dilution in immunohistochemical analysis.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)

        Formalin-fixed, paraffin-embedded human liver tissue stained for N Cadherin with ab225719 at 1/100 dilution in immunohistochemical analysis.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)

        Formalin-fixed, paraffin-embedded human adrenal gland tissue stained for N Cadherin with ab225719 at 1/100 dilution in immunohistochemical analysis.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)

        Formalin-fixed, paraffin-embedded human fallopian tube tissue stained for N Cadherin with ab225719 at 1/100 dilution in immunohistochemical analysis.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)

        Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for N Cadherin with ab225719 at 1/100 dilution in immunohistochemical analysis.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody [SP90] - C-terminal (ab225719)

        Formalin-fixed, paraffin-embedded human stomach tissue stained for N Cadherin with ab225719 at 1/100 dilution in immunohistochemical analysis.

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      Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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