AB76059

Anti-N Cadherin (phospho Y820) antibody

Name: Anti-N Cadherin (phospho Y820) antibody (ab76059) | Abcam
Brand: Abcam Limited
SKU: AB76059
Price: 535 USD
Availability: InStock
Rating: 3 (2 reviews)

(2 Reviews)

(8 Publications)

Rabbit Polyclonal N Cadherin phospho Y820 antibody. Suitable for WB and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human CDH2 pY820.

View Alternative Names

CD325, CDHN, NCAD, CDH2, Cadherin-2, CDw325, Neural cadherin, N-cadherin

1 Images

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Western blot - Anti-N Cadherin (phospho Y820) antibody (AB76059)

All lanes:

Western blot - Anti-N Cadherin (phospho Y820) antibody (ab76059) at 1/1000 dilution

Lane 1:

Human endothelial cells untreated

Lane 2:

Human endothelial cells treated with pervanadate (1 mM) for 30 min

Lane 3:

Human endothelial cells treated with pervanadate (1 mM) for 30 min with phospho-N-cadherin (Tyr-820) peptide

Lane 4:

Human endothelial cells treated with pervanadate (1 mM) for 30 min with phospho-N-cadherin (Tyr-860) peptide

Predicted band size: 99 kDa

Observed band size: 130 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human CDH2 pY820. The exact immunogen used to generate this antibody is proprietary information.

P19022

Specificity

ab76059 detects a 130 kDa band corresponding to N cadherin in western blots of serum-starved human endothelial cells treated with pervanadate, but it is not detected in untreated cells.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Do not aliquot this antibody.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab76059 was cross-adsorbed to phospho-N Cadherin (Tyr-860) and unphosphorylated N cadherin (Tyr-820) peptides before affinity purification using phospho-N cadherin (Tyr-820) peptide.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

N-Cadherin also known as CDH2 or neuronal cadherin is a calcium-dependent cell adhesion protein. It is characterized by its role in mediating intercellular connections primarily through its presence on neurons fibroblasts and certain epithelial cells. N-Cadherin has an approximate mass of 130 kDa. The protein is integral in forming homophilic interactions where N-Cadherin molecules on adjacent cells interact to establish robust cell-cell adhesion.

Biological function summary

N-Cadherin significantly influences cell-cell interaction and communication facilitating cellular adhesion and signal transduction. It is a vital component of adherens junctions contributing to tissue morphogenesis and stability. N-Cadherin interacts with other cytoplasmic proteins such as catenins forming a complex essential for linking the actin cytoskeleton to the cell membrane. This interaction affects cellular behaviors including migration and differentiation.

Pathways

N-Cadherin plays a significant role in the neural development and epithelial-to-mesenchymal transition (EMT) pathways important for development and cancer progression. It engages with related proteins such as beta-catenin which helps transduce signals within these pathways. N-Cadherin's interactions within these pathways highlight its role in maintaining multicellular structure and signaling processes important for development and pathogenesis.

N-Cadherin is associated with cancer and heart disease. In cancer particularly in the context of the EMT N-Cadherin facilitates tumor progression and metastasis cooperating with proteins like Twist and Snail to promote these processes. In heart disease alterations in N-Cadherin expression and function can impact cardiac muscle integrity and syncytium highlighting potential interactions with connexin43 to maintain cardiac function. Understanding these associations provides insights into therapeutic targets for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium-dependent cell adhesion protein; preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone : upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. Plays a role in cell-to-cell junction formation between pancreatic beta cells and neural crest stem (NCS) cells, promoting the formation of processes by NCS cells (By similarity). Required for proper neurite branching. Required for pre- and postsynaptic organization (By similarity). CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. May promote axon outgrowth and motor fiber repair via DSP-mediated recruitment to outgrowth tips (By similarity).

See full target information CDH2 pY820

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

NPJ Regenerative medicine 10:17 PubMed40175378

2025

Preclinical efficacy and safety of AAVrh10-based plakophilin-2 gene therapy (LX2020) as a treatment for arrhythmogenic cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Zhang,Erika Joana Gutierrez-Lara,Aryanne Do,Lena Nguyen,Anju Nair,Nithya Selvan,Tim Fenn,Eric Adler,Richie Khanna,Farah Sheikh

iScience 28:111989 PubMed40083715

2025

TAF7 directly targets SAA1 to enhance triple-negative breast cancer metastasis via phosphorylating E-cadherin and N-cadherin.

Applications

Unspecified application

Species

Unspecified reactive species

Wanjun Zhang,Jun Wang,Hanning Li,Xue Zhang,Dunjie Yao,Huimin Zhang,Xinhong Zhou,Jiaqi Nie,Tongxing Lai,Haichuan Zhu,Yiping Gong,Yoshimasa Tanaka,Xingrui Li,Xinghua Liao,Li Su

Cell adhesion & migration 18:1-12 PubMed38831518

2024

Knockdown of PIK3R6 impedes the onset and advancement of clear cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Yang,Xiaoni Zhong,Xiaoling Gao,Wenyi Xie,Yaokai Chen,Yuanjiang Liao,Peilin Zhang

Heliyon 10:e31520 PubMed38828336

2024

ANGPTL3 diminishes the resistance of ovarian cancer to paclitaxel by blocking the PI3K-AKT-mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Dandan Wu,Jia Liu,Xin Yang,Zhifen Wu,Tingzhao Wang,Meiqin Xiao

The clinical respiratory journal 18:e13770 PubMed38783645

2024

ZEB1-AS1 mediates bone metastasis through targeting miR-320b/BMPR1A axis in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Nianxi Tan,Junyi Tang,Guang Chen,Weilin Jiang,Zhiqin Liu

Frontiers in oncology 12:948023 PubMed35924156

2022

Apogossypolone Inhibits Cell Proliferation and Epithelial-Mesenchymal Transition in Cervical Cancer Activating DKK3.

Applications

Unspecified application

Species

Unspecified reactive species

Yuling Li,Jinfeng Qu,Lu Liu,Yu Sun,Junhua Zhang,Sai Han,Youzhong Zhang

Cellular & molecular biology letters 27:39 PubMed35578166

2022

Identification and validation of an eight-lncRNA signature that predicts prognosis in patients with esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jinfeng Zhang,Xiaodong Ling,Chengyuan Fang,Jianqun Ma

Oncology letters 22:773 PubMed34589152

2021

Long non-coding RNA TMCC1-AS1 predicts poor prognosis and accelerates epithelial-mesenchymal transition in liver cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Chen,Na Su,Guiying Li,Yanfeng Shen,Xiaoting Duan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-N Cadherin (phospho Y820) antibody

Western blot - Anti-N Cadherin (phospho Y820) antibody (AB76059)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise