AB119752

Anti-N Cadherin (phospho Y860) antibody

Name: Anti-N Cadherin (phospho Y860) antibody (ab119752) | Abcam
Brand: Abcam Limited
SKU: AB119752

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(4 Publications)

Rabbit Polyclonal N Cadherin phospho Y860 antibody. Suitable for ICC, WB and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human CDH2 pY860.

View Alternative Names

CD325, CDHN, NCAD, CDH2, Cadherin-2, CDw325, Neural cadherin, N-cadherin

2 Images

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Immunocytochemistry - Anti-N Cadherin (phospho Y860) antibody (AB119752)

ab119752 at 1/200 dilution staining N Cadherin (phospho Y860) in Mouse C2C12 cells, treated with pervanadate (right). The cells on the left were not treated with pervanadate prior to analysis.
Detection used an appropriate secondary antibody conjugated to Cy3.

Unknown

Western blot - Anti-N Cadherin (phospho Y860) antibody (AB119752)

All lanes:

Western blot - Anti-N Cadherin (phospho Y860) antibody (ab119752) at 1/1000 dilution

Lanes 1, 3, 5 and 7:

Human endothelial cells, untreated

Lanes 2, 4 and 8:

Human endothelial cells, treated with pervanadate (1 mM) for 30 min

Lane 6:

Human endothelial cells treated with pervanadate (1 mM) for 30 min

Predicted band size: 99 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC, WB

applications

Immunogen

Synthetic Peptide within Human CDH2 pY860. The exact immunogen used to generate this antibody is proprietary information.

P19022

Specificity

Immunogen sequence is conserved in Human E Cadherin (Y835), P Cadherin (Y782), R Cadherin (Y870) and has significant homology to VE Cadherin (Y733).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/200", "ICC-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Do not aliquot.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

ab119752 was cross-adsorbed to phospho-N-Cadherin (Tyr-820) and unphosphorylated N-cadherin (Tyr-860) peptides beforeaffinity purification using phospho-N-cadherin (Tyr-860) peptide.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

N-Cadherin also known as CDH2 or neuronal cadherin is a calcium-dependent cell adhesion protein. It is characterized by its role in mediating intercellular connections primarily through its presence on neurons fibroblasts and certain epithelial cells. N-Cadherin has an approximate mass of 130 kDa. The protein is integral in forming homophilic interactions where N-Cadherin molecules on adjacent cells interact to establish robust cell-cell adhesion.

Biological function summary

N-Cadherin significantly influences cell-cell interaction and communication facilitating cellular adhesion and signal transduction. It is a vital component of adherens junctions contributing to tissue morphogenesis and stability. N-Cadherin interacts with other cytoplasmic proteins such as catenins forming a complex essential for linking the actin cytoskeleton to the cell membrane. This interaction affects cellular behaviors including migration and differentiation.

Pathways

N-Cadherin plays a significant role in the neural development and epithelial-to-mesenchymal transition (EMT) pathways important for development and cancer progression. It engages with related proteins such as beta-catenin which helps transduce signals within these pathways. N-Cadherin's interactions within these pathways highlight its role in maintaining multicellular structure and signaling processes important for development and pathogenesis.

N-Cadherin is associated with cancer and heart disease. In cancer particularly in the context of the EMT N-Cadherin facilitates tumor progression and metastasis cooperating with proteins like Twist and Snail to promote these processes. In heart disease alterations in N-Cadherin expression and function can impact cardiac muscle integrity and syncytium highlighting potential interactions with connexin43 to maintain cardiac function. Understanding these associations provides insights into therapeutic targets for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium-dependent cell adhesion protein; preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone : upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. Plays a role in cell-to-cell junction formation between pancreatic beta cells and neural crest stem (NCS) cells, promoting the formation of processes by NCS cells (By similarity). Required for proper neurite branching. Required for pre- and postsynaptic organization (By similarity). CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. May promote axon outgrowth and motor fiber repair via DSP-mediated recruitment to outgrowth tips (By similarity).

See full target information CDH2 pY860

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of diabetes investigation 15:300-314 PubMed38149724

2023

Cordyceps cicadae polysaccharides attenuate diabetic nephropathy via the miR-30a-3p/TRIM16 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Zheng,Qin Xu,Yiwen Wang,Yifei Zhong,Rong Zhu

Molecular brain 13:118 PubMed32859226

2020

mGluR5 regulates REST/NRSF signaling through N-cadherin/β-catenin complex in Huntington's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jéssica M de Souza,Khaled S Abd-Elrahman,Fabiola M Ribeiro,Stephen S G Ferguson

Oncotarget 8:24902-24914 PubMed28212546

2017

Precursor N-cadherin mediates glial cell line-derived neurotrophic factor-promoted human malignant glioma.

Applications

Unspecified application

Species

Human

Ye Xiong,Liyun Liu,Shuang Zhu,Baole Zhang,Yuxia Qin,Ruiqin Yao,Hao Zhou,Dian Shuai Gao

Andrology 3:569-81 PubMed25873562

2015

Flutamide alters the distribution of c-Src and affects the N-cadherin-β-catenin complex in the seminiferous epithelium of adult rat.

Applications

Unspecified application

Species

Unspecified reactive species

M Zarzycka,K Chojnacka,D D Mruk,E Gorowska,A Hejmej,M Kotula-Balak,L Pardyak,B Bilinska

View all publications

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Product promise

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Anti-N Cadherin (phospho Y860) antibody

Immunocytochemistry - Anti-N Cadherin (phospho Y860) antibody (AB119752)

Western blot - Anti-N Cadherin (phospho Y860) antibody (AB119752)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise