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Rabbit Polyclonal N Cadherin phospho Y860 antibody. Suitable for ICC, WB and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CDH2 phospho Y860.

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Images

Immunocytochemistry - Anti-N Cadherin (phospho Y860) antibody (AB119752), expandable thumbnail
  • Western blot - Anti-N Cadherin (phospho Y860) antibody (AB119752), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human CDH2 phospho Y860. The exact immunogen used to generate this antibody is proprietary information. Database link P19022

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICCWB
Human
Expected
Tested
Mouse
Tested
Expected

Tested
Tested

Species

Mouse

Dilution info

1/200

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Calcium-dependent cell adhesion protein; preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. Plays a role in cell-to-cell junction formation between pancreatic beta cells and neural crest stem (NCS) cells, promoting the formation of processes by NCS cells (By similarity). Required for proper neurite branching. Required for pre- and postsynaptic organization (By similarity). CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.

Alternative names

Recommended products

Rabbit Polyclonal N Cadherin phospho Y860 antibody. Suitable for ICC, WB and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CDH2 phospho Y860.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human CDH2 phospho Y860. The exact immunogen used to generate this antibody is proprietary information. Database link P19022
Purification technique

Affinity purification Protein A

Specificity

Immunogen sequence is conserved in Human E Cadherin (Y835), P Cadherin (Y782), R Cadherin (Y870) and has significant homology to VE Cadherin (Y733).

Concentration
Loading...
Purification notes

ab119752 was cross-adsorbed to phospho-N-Cadherin (Tyr-820) and unphosphorylated N-cadherin (Tyr-860) peptides beforeaffinity purification using phospho-N-cadherin (Tyr-860) peptide.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

Notes

Do not aliquot.

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

N-Cadherin also known as CDH2 or neuronal cadherin is a calcium-dependent cell adhesion protein. It is characterized by its role in mediating intercellular connections primarily through its presence on neurons fibroblasts and certain epithelial cells. N-Cadherin has an approximate mass of 130 kDa. The protein is integral in forming homophilic interactions where N-Cadherin molecules on adjacent cells interact to establish robust cell-cell adhesion.

Biological function summary

N-Cadherin significantly influences cell-cell interaction and communication facilitating cellular adhesion and signal transduction. It is a vital component of adherens junctions contributing to tissue morphogenesis and stability. N-Cadherin interacts with other cytoplasmic proteins such as catenins forming a complex essential for linking the actin cytoskeleton to the cell membrane. This interaction affects cellular behaviors including migration and differentiation.

Pathways

N-Cadherin plays a significant role in the neural development and epithelial-to-mesenchymal transition (EMT) pathways important for development and cancer progression. It engages with related proteins such as beta-catenin which helps transduce signals within these pathways. N-Cadherin's interactions within these pathways highlight its role in maintaining multicellular structure and signaling processes important for development and pathogenesis.

Associated diseases and disorders

N-Cadherin is associated with cancer and heart disease. In cancer particularly in the context of the EMT N-Cadherin facilitates tumor progression and metastasis cooperating with proteins like Twist and Snail to promote these processes. In heart disease alterations in N-Cadherin expression and function can impact cardiac muscle integrity and syncytium highlighting potential interactions with connexin43 to maintain cardiac function. Understanding these associations provides insights into therapeutic targets for these conditions.

Product promise

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2 product images

  • Immunocytochemistry - Anti-N Cadherin (phospho Y860) antibody (ab119752), expandable thumbnail

    Immunocytochemistry - Anti-N Cadherin (phospho Y860) antibody (ab119752)

    ab119752 at 1/200 dilution staining N Cadherin (phospho Y860) in Mouse C2C12 cells, treated with pervanadate (right). The cells on the left were not treated with pervanadate prior to analysis.
    Detection used an appropriate secondary antibody conjugated to Cy3.

  • Western blot - Anti-N Cadherin (phospho Y860) antibody (ab119752), expandable thumbnail

    Western blot - Anti-N Cadherin (phospho Y860) antibody (ab119752)

    Lanes 1 - 2: Anti-N Cadherin (Cytoplasmic) at 1/1000 dilution

    Lanes 3 - 4: Anti-N Cadherin(phospho Y820) at 1/1000 dilution

    Lanes 5 - 6: Anti-N Cadherin (a.a. 853-864) at 1/1000 dilution

    Lanes 7 - 8: Western blot - Anti-N Cadherin (phospho Y860) antibody (ab119752) at 1/1000 dilution

    Lanes 1, 3, 5 and 7: Human endothelial cells, untreated

    Lanes 2, 4 and 8: Human endothelial cells, treated with pervanadate (1 mM) for 30 min

    Lane 6: Human endothelial cells treated with pervanadate (1 mM) for 30 min

    Predicted band size: 99 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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