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AB126202

Anti-N-ethylmaleimide-sensitive fusion protein antibody

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(2 Publications)

Rabbit Polyclonal N-ethylmaleimide-sensitive fusion protein antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NSF aa 350-600.

View Alternative Names

Vesicle-fusing ATPase, N-ethylmaleimide-sensitive fusion protein, Vesicular-fusion protein NSF, NEM-sensitive fusion protein, NSF

3 Images
Western blot - Anti-N-ethylmaleimide-sensitive fusion protein antibody (AB126202)
  • WB

Unknown

Western blot - Anti-N-ethylmaleimide-sensitive fusion protein antibody (AB126202)

7.5% SDS PAGE

All lanes:

Western blot - Anti-N-ethylmaleimide-sensitive fusion protein antibody (ab126202) at 1/1000 dilution

All lanes:

IMR32 whole cell lysate at 30 µg

Predicted band size: 83 kDa

false

Western blot - Anti-N-ethylmaleimide-sensitive fusion protein antibody (AB126202)
  • WB

Supplier Data

Western blot - Anti-N-ethylmaleimide-sensitive fusion protein antibody (AB126202)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-N-ethylmaleimide-sensitive fusion protein antibody (ab126202) at 1/1000 dilution

All lanes:

Rat brain tissue extract at 50 µg

Predicted band size: 83 kDa

false

Western blot - Anti-N-ethylmaleimide-sensitive fusion protein antibody (AB126202)
  • WB

Supplier Data

Western blot - Anti-N-ethylmaleimide-sensitive fusion protein antibody (AB126202)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-N-ethylmaleimide-sensitive fusion protein antibody (ab126202) at 1/1000 dilution

All lanes:

Mouse brain tissue extract at 50 µg

Predicted band size: 83 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human NSF aa 350-600. The exact immunogen used to generate this antibody is proprietary information.

P46459

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

N-ethylmaleimide-sensitive fusion protein (NSF) is an important component in the cellular machinery. It unwinds homo-oligomer complexes enabling membrane fusion in cells. NSF also goes by the name of NEM-sensitive factor. It has a mass of about 82 kDa. It is expressed in most tissues where membrane fusion processes occur such as neurons liver and other secretory cells.
Biological function summary

NSF plays a central role in ensuring proper vesicular transport. It is part of the SNARE complex which facilitates the fusion of vesicles with their target membranes. Interaction with small regulatory proteins like SNAPs (Soluble NSF Attachment Proteins) ensures NSF activity in recycling SNARE proteins after vesicle fusion. This process is critical for neurotransmitter release in neuronal synapses and for enzymatic secretion in glandular tissues.

Pathways

NSF contributes significantly to intracellular vesicle trafficking and neurotransmitter release pathways. In these pathways proteins like VAMP synaptobrevin and SNAP-25 interact with NSF to maintain the continuity of vesicle-mediated transport. NSF activity modulates SNARE-mediated vesicle fusion thereby allowing efficient cargo transfer between cellular compartments.

NSF has connections to neurological disorders and certain protein trafficking diseases. Mutations in NSF or dysfunction of its SNARE partners can link to neurodegenerative diseases like Alzheimer’s disease. Proteins like the amyloid-beta precursor protein can also interact aberrantly with NSF pathway components exacerbating disease states. Additionally improper NSF function may relate to cystic fibrosis due to issues in vesicular transport affecting CFTR protein localization.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling (By similarity).
See full target information NSF
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

iScience 25:104989 PubMed36093041

2022

PRG-1 prevents neonatal stimuli-induced persistent hyperalgesia and memory dysfunction via NSF/Glu/GluR2 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xingfeng Liu,Site Li,Wenyu Zhang,Zhuo Xie,Jingxin He,Xuanwei Zhang,Shouyang Yu,Song Cao,Tian Yu,Zhi Xiao

PloS one 13:e0206883 PubMed30481179

2018

Deletion of Pr72 causes cardiac developmental defects in Zebrafish.

Applications

Unspecified application

Species

Unspecified reactive species

Guibo Song,Mingjun Han,Zuhua Li,Xuedong Gan,Xiaowen Chen,Jie Yang,Sufang Dong,Ming Yan,Jun Wan,Yanggan Wang,Zhuliang Huang,Zhan Yin,Fang Zheng
View all publications
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Product promise

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