Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] - BSA and Azide free

• IHC

Supplier Data

Immunohistochemistry - Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] - BSA and Azide free (AB302789)

This data was developed using ab302788, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling N-ethylmaleimide-sensitive fusion protein with ab302788 at 1/500 (0.944 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Cytoplasmic staining on rat cerebrum. The section was incubated with ab302788 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC

Supplier Data

Immunohistochemistry - Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] - BSA and Azide free (AB302789)

This data was developed using ab302788, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling N-ethylmaleimide-sensitive fusion protein with ab302788 at 1/500 (0.944 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Cytoplasmic staining on mouse hippocampus. The section was incubated with ab302788 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC

Supplier Data

Immunohistochemistry - Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] - BSA and Azide free (AB302789)

This data was developed using ab302788, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat lung tissue labeling N-ethylmaleimide-sensitive fusion protein with ab302788 at 1/500 (0.944 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Negative control : No staining on rat lung. The section was incubated with ab302788 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• WB

Lab

Western blot - Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] - BSA and Azide free (AB302789)

This data was developed using ab302788, the same antibody clone in a different buffer formulation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/1000000.

All lanes:

Western blot - Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] (<a href='/en-us/products/primary-antibodies/n-ethylmaleimide-sensitive-fusion-protein-antibody-epr25190-1-ab302788'>ab302788</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse hippocampus tissue lysate at 20 µg

Lane 3:

Mouse heart tissue lysate at 20 µg

Lane 4:

Rat brain tissue lysate at 20 µg

Lane 5:

Rat hippocampus tissue lysate at 20 µg

Lane 6:

Rat heart tissue lysate at 20 µg

Lane 7:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

Lane 8:

PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 80 kDa

false

Exposure time: 40s