Rabbit Monoclonal N-ethylmaleimide-sensitive fusion protein antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat samples.
Images
![Western blot - Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] - BSA and Azide free (AB302789), expandable thumbnail](https://content.abcam.com/products/images/n-ethylmaleimide-sensitive-fusion-protein-antibody-epr25190-1-bsa-and-azide-free-ab302789--western-blot-img446021.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] - BSA and Azide free (AB302789)")
![Immunohistochemistry - Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] - BSA and Azide free (AB302789), expandable thumbnail](https://content.abcam.com/products/images/n-ethylmaleimide-sensitive-fusion-protein-antibody-epr25190-1-bsa-and-azide-free-ab302789--immunohistochemistry-img401330.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] - BSA and Azide free (AB302789)")
![Immunohistochemistry - Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] - BSA and Azide free (AB302789), expandable thumbnail](https://content.abcam.com/products/images/n-ethylmaleimide-sensitive-fusion-protein-antibody-epr25190-1-bsa-and-azide-free-ab302789--immunohistochemistry-img401329.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] - BSA and Azide free (AB302789)")
![Immunohistochemistry - Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] - BSA and Azide free (AB302789), expandable thumbnail](https://content.abcam.com/products/images/n-ethylmaleimide-sensitive-fusion-protein-antibody-epr25190-1-bsa-and-azide-free-ab302789--immunohistochemistry-img401328.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] - BSA and Azide free (AB302789)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | IHC-Fr | ICC/IF | IP | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Tested | Not recommended | Not recommended | Not recommended |
| Rat | Tested | Tested | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes This antibody may require protocol optimization (e.g., increased sample loading, reduced dilution, femtogram-level ECL substrates) for improved sensitivity in testing cell line lysate. Additionally, faint non-specific band close to target band may appear in low-expression tissues (e.g., heart); validation with controls is recommended. |
Species Rat | Dilution info - | Notes This antibody may require protocol optimization (e.g., increased sample loading, reduced dilution, femtogram-level ECL substrates) for improved sensitivity in testing cell line lysate. Additionally, faint non-specific band close to target band may appear in low-expression tissues (e.g., heart); validation with controls is recommended. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes - |
Species Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Target data
Function
Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack GRIA2 leads to influence GRIA2 membrane cycling (By similarity).
Alternative names
Skd2, Nsf, Vesicle-fusing ATPase, N-ethylmaleimide-sensitive fusion protein, Suppressor of K(+) transport growth defect 2, Vesicular-fusion protein NSF, NEM-sensitive fusion protein, Protein SKD2
Recommended products
Rabbit Monoclonal N-ethylmaleimide-sensitive fusion protein antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR25190-1
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
N-ethylmaleimide-sensitive fusion protein (NSF) is an important component in the cellular machinery. It unwinds homo-oligomer complexes enabling membrane fusion in cells. NSF also goes by the name of NEM-sensitive factor. It has a mass of about 82 kDa. It is expressed in most tissues where membrane fusion processes occur such as neurons liver and other secretory cells.
- Biological function summary
NSF plays a central role in ensuring proper vesicular transport. It is part of the SNARE complex which facilitates the fusion of vesicles with their target membranes. Interaction with small regulatory proteins like SNAPs (Soluble NSF Attachment Proteins) ensures NSF activity in recycling SNARE proteins after vesicle fusion. This process is critical for neurotransmitter release in neuronal synapses and for enzymatic secretion in glandular tissues.
- Pathways
NSF contributes significantly to intracellular vesicle trafficking and neurotransmitter release pathways. In these pathways proteins like VAMP synaptobrevin and SNAP-25 interact with NSF to maintain the continuity of vesicle-mediated transport. NSF activity modulates SNARE-mediated vesicle fusion thereby allowing efficient cargo transfer between cellular compartments.
- Associated diseases and disorders
NSF has connections to neurological disorders and certain protein trafficking diseases. Mutations in NSF or dysfunction of its SNARE partners can link to neurodegenerative diseases like Alzheimer’s disease. Proteins like the amyloid-beta precursor protein can also interact aberrantly with NSF pathway components exacerbating disease states. Additionally improper NSF function may relate to cystic fibrosis due to issues in vesicular transport affecting CFTR protein localization.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] - BSA and Azide free (ab302789), expandable thumbnail](https://content.abcam.com/products/images/n-ethylmaleimide-sensitive-fusion-protein-antibody-epr25190-1-bsa-and-azide-free-ab302789--western-blot-img446021.jpg "Western blot - Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] - BSA and Azide free (ab302789)")
Western blot - Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] - BSA and Azide free (ab302789)
N-ethylmaleimide-sensitive fusion protein Western blot staining using rabbit Anti-N-ethylmaleimide-sensitive fusion protein antibody
This data was developed using Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] ab302788, the same antibody clone in a different buffer formulation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/1000000.
All lanes: Western blot - Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] (Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] ab302788) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Mouse hippocampus tissue lysate at 20 µg
Lane 3: Mouse heart tissue lysate at 20 µg
Lane 4: Rat brain tissue lysate at 20 µg
Lane 5: Rat hippocampus tissue lysate at 20 µg
Lane 6: Rat heart tissue lysate at 20 µg
Lane 7: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg
Lane 8: PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 80 kDa
Exposure time: 40s
![Immunohistochemistry - Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] - BSA and Azide free (ab302789), expandable thumbnail](https://content.abcam.com/products/images/n-ethylmaleimide-sensitive-fusion-protein-antibody-epr25190-1-bsa-and-azide-free-ab302789--immunohistochemistry-img401330.jpg "Immunohistochemistry - Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] - BSA and Azide free (ab302789)")
Immunohistochemistry - Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] - BSA and Azide free (ab302789)
This data was developed using Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] ab302788, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat lung tissue labeling N-ethylmaleimide-sensitive fusion protein with Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] ab302788 at 1/500 (0.944 ug/ml) followed by a LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Negative control: No staining on rat lung. The section was incubated with Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] ab302788 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
![Immunohistochemistry - Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] - BSA and Azide free (ab302789), expandable thumbnail](https://content.abcam.com/products/images/n-ethylmaleimide-sensitive-fusion-protein-antibody-epr25190-1-bsa-and-azide-free-ab302789--immunohistochemistry-img401329.jpg "Immunohistochemistry - Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] - BSA and Azide free (ab302789)")
Immunohistochemistry - Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] - BSA and Azide free (ab302789)
This data was developed using Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] ab302788, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling N-ethylmaleimide-sensitive fusion protein with Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] ab302788 at 1/500 (0.944 ug/ml) followed by a LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Cytoplasmic staining on mouse hippocampus. The section was incubated with Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] ab302788 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
![Immunohistochemistry - Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] - BSA and Azide free (ab302789), expandable thumbnail](https://content.abcam.com/products/images/n-ethylmaleimide-sensitive-fusion-protein-antibody-epr25190-1-bsa-and-azide-free-ab302789--immunohistochemistry-img401328.jpg "Immunohistochemistry - Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] - BSA and Azide free (ab302789)")
Immunohistochemistry - Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] - BSA and Azide free (ab302789)
This data was developed using Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] ab302788, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling N-ethylmaleimide-sensitive fusion protein with Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] ab302788 at 1/500 (0.944 ug/ml) followed by a LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Cytoplasmic staining on rat cerebrum. The section was incubated with Anti-N-ethylmaleimide-sensitive fusion protein antibody [EPR25190-1] ab302788 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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