AB24193

Anti-n-Myc/MYCN antibody

Name: Anti-n-Myc/MYCN antibody (ab24193) | Abcam
Brand: Abcam Limited
SKU: AB24193
Price: 485 USD
Availability: InStock
Rating: 4 (5 reviews)

(5 Reviews)

(16 Publications)

Rabbit Polyclonal N-Myc/MYCN antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 16 publications.

View Alternative Names

BHLHE37, NMYC, MYCN, N-myc proto-oncogene protein, Class E basic helix-loop-helix protein 37, bHLHe37

2 Images

Lab

Western blot - Anti-n-Myc/MYCN antibody (AB24193)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab24193 overnight at 4°C. Antibody binding was detected using VHH Single Domain Anti-Rabbit IgG Fc (HRP) (ab191866), and visualised using ECL development solution ab133406.

All lanes:

Western blot - Anti-n-Myc/MYCN antibody (ab24193) at 1 µg/mL

Lane 1:

Heart (Human) Whole Cell Lysate - fetal normal tissue at 10 µg

Lane 2:

Heart (Mouse) Tissue Lysate at 10 µg

Lane 3:

Skeletal Muscle (Mouse) Tissue Lysate at 10 µg

Secondary

All lanes:

Western blot - VHH Single Domain Anti-Rabbit IgG Fc (HRP) (<a href='/en-us/products/secondary-antibodies/vhh-single-domain-rabbit-igg-fc-hrp-ab191866'>ab191866</a>) at 1/50000 dilution

Predicted band size: 50 kDa

Observed band size: 190 kDa,49 kDa

true

Exposure time: 30s

Unknown

Western blot - Anti-n-Myc/MYCN antibody (AB24193)

All lanes:

Western blot - Anti-n-Myc/MYCN antibody (ab24193) at 1 µg/mL

Lane 1:

Heart (Human) Whole Cell Lysate - fetal normal tissue (<a href='/en-us/products/unavailable/heart-human-whole-cell-lysate-fetal-normal-tissue-ab29432'>ab29432</a>) at 20 µg

Lane 2:

Heart (Human) Nuclear Lysate - fetal normal tissue (<a href='/en-us/products/unavailable/heart-human-nuclear-lysate-fetal-normal-tissue-ab29428'>ab29428</a>) at 20 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

Predicted band size: 50 kDa

Observed band size: 25 kDa,33 kDa,39 kDa,49 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Primates": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

N-Myc also known as MYCN is a transcription factor encoded by the MYCN gene. It plays an important role in cell proliferation and differentiation. The n-Myc protein is part of the Myc family of transcription factors including c-Myc and c-Myb. n-Myc has a molecular weight of approximately 64 kDa. This protein expresses predominantly in developing tissues particularly in the brain but its expression is seen in other tissues during embryogenesis as well. This characteristic makes it important in early development and organ formation.

Biological function summary

N-Myc/MYCN functions in gene regulation by binding to E-box sequences in DNA facilitating transcription of genes essential for cellular growth and metabolism. n-Myc often forms a heterodimer with the MAX protein which enhances its DNA-binding capacity and transcriptional activity. This activity influences processes like apoptosis cell differentiation and cell cycle progression. The ncm mouse model a genetically engineered mouse with MYCN overexpression demonstrates the effects of deregulated n-Myc expression.

Pathways

MYCN significantly impacts the p53 signaling pathway and the Wnt signaling pathway. In the p53 signaling pathway n-Myc interacts with p53 and MDM2 regulating cell cycle checkpoints and apoptosis. In the Wnt pathway MYCN interplays with molecules like β-catenin to influence cell division and differentiation. These interactions highlight the role of n-Myc in maintaining cellular homeostasis and response to external signals that affect growth and division.

N-Myc is closely linked to neuroblastoma and medulloblastoma aggressive forms of pediatric cancers. MYCN amplification often indicates a poor prognosis in neuroblastoma playing a role in tumor progression and resistance to therapy. In medulloblastoma overexpression of n-Myc partners with proteins like Aurora A kinase to promote oncogenesis. Targeting these protein interactions offers potential therapeutic strategies for these MYCN-related cancers.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Positively regulates the transcription of MYCNOS in neuroblastoma cells.

See full target information MYCN

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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

International journal of genomics 2024:6594426 PubMed39465005

2024

EIF2S1 Silencing Impedes Neuroblastoma Development Through GPX4 Inactivation and Ferroptosis Induction.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Li,Yunhui Wang,Shubin Liang,Tingdong Yuan,Jing Liu

Cancer cell international 24:153 PubMed38685086

2024

Exosome-mediated secretion of miR-127-3p regulated by RAB27A accelerates metastasis in renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Dae Hyun Song,Jong Sil Lee,Jeong-Hee Lee,Dong Chul Kim,Jung Wook Yang,Min Hye Kim,Ji Min Na,Hyun-Kyung Cho,Jiyun Yoo,Hyo Jung An

Cancers 14: PubMed35681607

2022

Extracellular Vesicle-Based Method for Detecting Amplification Status of Pediatric Neuroblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jirawan Panachan,Napat Rojsirikulchai,Nutkridta Pongsakul,Ladawan Khowawisetsut,Pongpak Pongphitcha,Teerapong Siriboonpiputtana,Takol Chareonsirisuthigul,Pitichai Phornsarayuth,Nisakorn Klinkulab,Natini Jinawath,Wararat Chiangjong,Usanarat Anurathapan,Kovit Pattanapanyasat,Suradej Hongeng,Somchai Chutipongtanate

Translational oncology 14:101149 PubMed34118691

2021

Anti-tumor effects of rigosertib in high-risk neuroblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Katarzyna Radke,Karin Hansson,Jonas Sjölund,Magdalena Wolska,Jenny Karlsson,Javanshir Esfandyari,Kristian Pietras,Kristina Aaltonen,David Gisselsson,Daniel Bexell

Cancer cell 36:302-318.e7 PubMed31474569

2019

Single-Cell Transcriptomics in Medulloblastoma Reveals Tumor-Initiating Progenitors and Oncogenic Cascades during Tumorigenesis and Relapse.

Applications

Unspecified application

Species

Unspecified reactive species

Liguo Zhang,Xuelian He,Xuezhao Liu,Feng Zhang,L Frank Huang,Andrew S Potter,Lingli Xu,Wenhao Zhou,Tao Zheng,Zaili Luo,Kalen P Berry,Allison Pribnow,Stephanie M Smith,Christine Fuller,Blaise V Jones,Maryam Fouladi,Rachid Drissi,Zeng-Jie Yang,W Clay Gustafson,Marc Remke,Scott L Pomeroy,Emily J Girard,James M Olson,A Sorana Morrissy,Maria C Vladoiu,Jiao Zhang,Weidong Tian,Mei Xin,Michael D Taylor,S Steven Potter,Martine F Roussel,William A Weiss,Q Richard Lu

Oncology reports : PubMed31524241

2019

β2‑adrenergic receptor signaling promotes neuroblastoma cell proliferation by activating autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Deng,Ping Jiang,Tianyou Yang,Mao Huang,Jinye Xie,Chuanghua Luo,Weiwei Qi,Ti Zhou,Zhonghan Yang,Yan Zou,Guoquan Gao,Xia Yang

Experimental and therapeutic medicine 17:3883-3890 PubMed31007733

2019

BMP4 and Neuregulin regulate the direction of mouse neural crest cell differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Shunqin Zhu,Wanhong Liu,Han-Fei Ding,Hongjuan Cui,Liqun Yang

Brain research 1704:219-228 PubMed30321496

2018

FGF represses metastasis of neuroblastoma regulated by MYCN and TGF-β1 induced LMO1 via control of let-7 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Hui Wang,Hai-Ying Wu,Jian Gao,Xu-Hui Wang,Tian-Hui Gao,Shu-Feng Zhang

Molecular medicine reports 18:4595-4602 PubMed30221657

2018

Huwe1 is a novel mediator of protection of neural progenitor L2.3 cells against oxygen‑glucose deprivation injury.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoqin Jiang,Jiyun Yang,Hedong Li,Yi Qu,Wenming Xu,Haiyan Yu,Yu Tong

Molecular medicine reports 18:377-384 PubMed29749516

2018

Downregulation of N‑Myc inhibits neuroblastoma cell growth via the Wnt/β‑catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yingge Wang,Shan Gao,Weiguang Wang,Yuting Xia,Jingyan Liang

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-n-Myc/MYCN antibody

Western blot - Anti-n-Myc/MYCN antibody (AB24193)

Western blot - Anti-n-Myc/MYCN antibody (AB24193)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (16)

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