Rabbit Recombinant Monoclonal N-Myc/MYCN antibody. Suitable for IP, ChIP, WB and reacts with Human samples. Cited in 1 publication.
Images
![ChIP - Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - ChIP Grade (AB227822), expandable thumbnail](https://content.abcam.com/products/images/n-myc-mycn-antibody-epr18982-8r-3-chip-grade-ab227822--chip-img110234.jpg/_jcr_content/renditions/webp-475.webp "ChIP - Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - ChIP Grade (AB227822)")
![Immunoprecipitation - Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - ChIP Grade (AB227822), expandable thumbnail](https://content.abcam.com/products/images/n-myc-mycn-antibody-epr18982-8r-3-chip-grade-ab227822--immunoprecipitation-img4304.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - ChIP Grade (AB227822)")
![Western blot - Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - ChIP Grade (AB227822), expandable thumbnail](https://content.abcam.com/products/images/n-myc-mycn-antibody-epr18982-8r-3-chip-grade-ab227822--western-blot-img9447.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - ChIP Grade (AB227822)")
Publications
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- Frontiers in medicine 9:9429912022A prognosis model for clear cell renal cell carcinoma based on four necroptosis-related genes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiangmin Qiu,Yanze Li,Ye Zhang,Yanguang Hou,Juncheng Hu,Lei Wang,Zhiyuan Chen,Yourong Lei,Yang Du,Xiuheng LiuPubMed 36016998
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 49% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | ChIP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Not recommended | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/30 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ChIP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg for 25 µg chromatin | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
Positively regulates the transcription of MYCNOS in neuroblastoma cells.
Alternative names
BHLHE37, NMYC, MYCN, N-myc proto-oncogene protein, Class E basic helix-loop-helix protein 37, bHLHe37
Recommended products
Rabbit Recombinant Monoclonal N-Myc/MYCN antibody. Suitable for IP, ChIP, WB and reacts with Human samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 49% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR18982-8R-3
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
N-Myc also known as MYCN is a transcription factor encoded by the MYCN gene. It plays an important role in cell proliferation and differentiation. The n-Myc protein is part of the Myc family of transcription factors including c-Myc and c-Myb. n-Myc has a molecular weight of approximately 64 kDa. This protein expresses predominantly in developing tissues particularly in the brain but its expression is seen in other tissues during embryogenesis as well. This characteristic makes it important in early development and organ formation.
- Biological function summary
N-Myc/MYCN functions in gene regulation by binding to E-box sequences in DNA facilitating transcription of genes essential for cellular growth and metabolism. n-Myc often forms a heterodimer with the MAX protein which enhances its DNA-binding capacity and transcriptional activity. This activity influences processes like apoptosis cell differentiation and cell cycle progression. The ncm mouse model a genetically engineered mouse with MYCN overexpression demonstrates the effects of deregulated n-Myc expression.
- Pathways
MYCN significantly impacts the p53 signaling pathway and the Wnt signaling pathway. In the p53 signaling pathway n-Myc interacts with p53 and MDM2 regulating cell cycle checkpoints and apoptosis. In the Wnt pathway MYCN interplays with molecules like β-catenin to influence cell division and differentiation. These interactions highlight the role of n-Myc in maintaining cellular homeostasis and response to external signals that affect growth and division.
- Associated diseases and disorders
N-Myc is closely linked to neuroblastoma and medulloblastoma aggressive forms of pediatric cancers. MYCN amplification often indicates a poor prognosis in neuroblastoma playing a role in tumor progression and resistance to therapy. In medulloblastoma overexpression of n-Myc partners with proteins like Aurora A kinase to promote oncogenesis. Targeting these protein interactions offers potential therapeutic strategies for these MYCN-related cancers.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![ChIP - Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - ChIP Grade (ab227822), expandable thumbnail](https://content.abcam.com/products/images/n-myc-mycn-antibody-epr18982-8r-3-chip-grade-ab227822--chip-img110234.jpg "ChIP - Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - ChIP Grade (ab227822)")
ChIP - Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - ChIP Grade (ab227822)
Chromatin was prepared from IMR-32 cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25 μg of chromatin, 5 μg of ab227822 (red), and 20 μl of Protein A/G sepharose beads. 5 μg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (SYBR green approach). Primers and probes are located in the first kb of the transcribed region.
![Immunoprecipitation - Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - ChIP Grade (ab227822), expandable thumbnail](https://content.abcam.com/products/images/n-myc-mycn-antibody-epr18982-8r-3-chip-grade-ab227822--immunoprecipitation-img4304.jpg "Immunoprecipitation - Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - ChIP Grade (ab227822)")
Immunoprecipitation - Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - ChIP Grade (ab227822)
n-Myc/MYCN was immunoprecipitated from 0.35 mg of IMR-32 (human neuroblastoma neuroblast cell line) whole cell lysate with ab227822 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab227822 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1000 dilution.
Lane 1: IMR-32 whole cell lysate 10 μg (Input).
Lane 2: ab227822 IP in IMR-32 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab227822 in IMR-32 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.The expression profile observed is consistent with what has been described in the literature (PMID: 17938259; PMID: 2657399).
All lanes: Immunoprecipitation - Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - ChIP Grade (ab227822)
Predicted band size: 50 kDa
Observed band size: 49-62 kDa
![Western blot - Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - ChIP Grade (ab227822), expandable thumbnail](https://content.abcam.com/products/images/n-myc-mycn-antibody-epr18982-8r-3-chip-grade-ab227822--western-blot-img9447.jpg "Western blot - Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - ChIP Grade (ab227822)")
Western blot - Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - ChIP Grade (ab227822)
Blocking and dilution buffer: 5% NFDM/TBST
The expression profile observed is consistent with what has been described in the literature (PMID: 11034201; PMID: 27197171; PMID: 23792191).
Negative control: HeLa (PMID: 27197171).
All lanes: Western blot - Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - ChIP Grade (ab227822) at 1/1000 dilution
Lane 1: IMR-32 (human neuroblastoma neuroblast cell line) whole cell lysate at 20 µg
Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 50 kDa
Observed band size: 62 kDa
Exposure time: 103s
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