Rabbit Polyclonal N WASP phospho Y256 antibody. Suitable for WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human Neural Wiskott-Aldrich syndrome protein phospho Y256.
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- Cell death & disease 11:1182020Targeting Src family kinase member Fyn by Saracatinib attenuated liver fibrosis in vitro and in vivo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuifang Du et. al.PubMed 32051399
- The Journal of physiology 596:3617-36352018Rho kinase collaborates with p21-activated kinase to regulate actin polymerization and contraction in airway smooth muscle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenwu Zhang et. al.PubMed 29746010
- The Journal of physiology 594:4879-9002016p21-Activated kinase (Pak) regulates airway smooth muscle contraction by regulating paxillin complexes that mediate actin polymerization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenwu Zhang et. al.PubMed 27038336
- The Journal of biological chemistry 288:4241-502012Neural Wiskott-Aldrich syndrome protein (N-WASP)-mediated p120-catenin interaction with Arp2-Actin complex stabilizes endothelial adherens junctions.Applications:WBReactive species:HumanCharu Rajput,Vidisha Kini,Monica Smith,Pascal Yazbeck,Alejandra Chavez,Tracy Schmidt,Wei Zhang,Nebojsa Knezevic,Yulia Komarova,Dolly MehtaPubMed 23212915
- The Journal of biological chemistry 287:33996-40082012The small GTPase RhoA regulates the contraction of smooth muscle tissues by catalyzing the assembly of cytoskeletal signaling complexes at membrane adhesion sites.
- Development (Cambridge, England) 139:3200-102012Focal adhesion kinase regulates actin nucleation and neuronal filopodia formation during axonal growth.Applications:WBReactive species:Unspecified reactive speciesMariola R Chacón,Ana I Navarro,German Cuesto,Isabel del Pino,Ricardo Scott,Miguel Morales,Beatriz RicoPubMed 22872087
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human Neural Wiskott-Aldrich syndrome protein phospho Y256. The exact immunogen used to generate this antibody is proprietary information. Database link O00401
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10000 | Notes The antibody detects a 63 or 65 kDa protein corresponding to phosphorylated N WASP on SDS PAGE immunoblots of A431, HeLa, Jurkat and endothelial cells treated with pervanadate. Weak bands are also observed at higher molecular weights after pervanadte treatment which may be due to low crossreactivity with phosphotyrosine. |
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Target data
Function
Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex (PubMed:16767080, PubMed:19366662, PubMed:19487689, PubMed:22847007, PubMed:22921828, PubMed:9422512). Involved in various processes, such as mitosis and cytokinesis, via its role in the regulation of actin polymerization (PubMed:19366662, PubMed:19487689, PubMed:22847007, PubMed:22921828, PubMed:9422512). Together with CDC42, involved in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia (PubMed:9422512). In addition to its role in the cytoplasm, also plays a role in the nucleus by regulating gene transcription, probably by promoting nuclear actin polymerization (PubMed:16767080). Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression (By similarity). Plays a role in dendrite spine morphogenesis (By similarity). Decreasing levels of DNMBP (using antisense RNA) alters apical junction morphology in cultured enterocytes, junctions curve instead of being nearly linear (PubMed:19767742).
Alternative names
Actin nucleation-promoting factor WASL, Neural Wiskott-Aldrich syndrome protein, N-WASP, WASL
Recommended products
Rabbit Polyclonal N WASP phospho Y256 antibody. Suitable for WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human Neural Wiskott-Aldrich syndrome protein phospho Y256.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human Neural Wiskott-Aldrich syndrome protein phospho Y256. The exact immunogen used to generate this antibody is proprietary information. Database link O00401
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
This antibody was cross absorbed to phosphotyrosine then affinity purified using phospho N WASP (Tyr 256) peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein N-WASP also known as neural Wiskott-Aldrich syndrome protein plays an important role in actin cytoskeleton dynamics. It weighs about 65 kDa and is widely expressed in diverse tissues but shows higher expression levels in the brain. In its mechanical function N-WASP regulates the polymerization of actin by activating the Arp2/3 complex. Through these interactions it helps in the formation of branched actin networks which are essential for cellular movement and shape.
- Biological function summary
N-WASP functions are critical in cell signaling endocytosis and neurite extension. N-WASP often acts within a complex and interacts with various proteins like Cdc42 to transmit signals from cell surface to the actin cytoskeleton. These activities position N-WASP as a fundamental component for cellular architecture and mobility impacting processes like immune response and neuronal development. Its interaction with Arp2/3 contributes to dynamic changes in cell movement and shape.
- Pathways
N-WASP plays a role in Rac and Cdc42 signaling pathways important for actin reorganization. Within these pathways it interacts with proteins such as Rho family GTPases influencing cytoskeletal arrangements. The proper functioning of these pathways is vital for numerous cellular processes including the migration of cells which affects tissue development and repair.
- Associated diseases and disorders
Alterations in N-WASP are linked to immunodeficiencies and neurological disorders. Mutations or dysfunction in N-WASP can lead to Wiskott-Aldrich syndrome characterized by immune defects and eczema due to its role in immune cell signaling. Additionally N-WASP's interaction with proteins such as WASP contributes to its involvement in neurodevelopmental disorders like autistic spectrum disorders reflecting the importance of this protein in maintaining normal cellular function.
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1 product image
Western blot - Anti-N WASP (phospho Y256) antibody (ab23395)
Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
All lanes: Western blot - Anti-N WASP (phospho Y256) antibody (ab23395) at 1/10000 dilution
Lane 1: Control A431 cells 20ug/lane
Lane 2: Pervanadate-treated A431 cells 20 ug/lane
Predicted band size: 55 kDa
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