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AB23395

Anti-N WASP (phospho Y256) antibody

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(6 Publications)

Rabbit Polyclonal N WASP phospho Y256 antibody. Suitable for WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human Neural Wiskott-Aldrich syndrome protein phospho Y256.

View Alternative Names

Actin nucleation-promoting factor WASL, Neural Wiskott-Aldrich syndrome protein, N-WASP, WASL

1 Images
Western blot - Anti-N WASP (phospho Y256) antibody (AB23395)
  • WB

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Western blot - Anti-N WASP (phospho Y256) antibody (AB23395)

Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.

All lanes:

Western blot - Anti-N WASP (phospho Y256) antibody (ab23395) at 1/10000 dilution

Lane 1:

Control A431 cells 20ug/lane

Lane 2:

Pervanadate-treated A431 cells 20 ug/lane

Predicted band size: 55 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human Neural Wiskott-Aldrich syndrome protein phospho Y256. The exact immunogen used to generate this antibody is proprietary information.

O00401

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p>The antibody detects a 63 or 65 kDa protein corresponding to phosphorylated N WASP on SDS PAGE immunoblots of A431, HeLa, Jurkat and endothelial cells treated with pervanadate. Weak bands are also observed at higher molecular weights after pervanadte treatment which may be due to low crossreactivity with phosphotyrosine.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
This antibody was cross absorbed to phosphotyrosine then affinity purified using phospho N WASP (Tyr 256) peptide.
Storage buffer
Preservative: 0.05% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein N-WASP also known as neural Wiskott-Aldrich syndrome protein plays an important role in actin cytoskeleton dynamics. It weighs about 65 kDa and is widely expressed in diverse tissues but shows higher expression levels in the brain. In its mechanical function N-WASP regulates the polymerization of actin by activating the Arp2/3 complex. Through these interactions it helps in the formation of branched actin networks which are essential for cellular movement and shape.
Biological function summary

N-WASP functions are critical in cell signaling endocytosis and neurite extension. N-WASP often acts within a complex and interacts with various proteins like Cdc42 to transmit signals from cell surface to the actin cytoskeleton. These activities position N-WASP as a fundamental component for cellular architecture and mobility impacting processes like immune response and neuronal development. Its interaction with Arp2/3 contributes to dynamic changes in cell movement and shape.

Pathways

N-WASP plays a role in Rac and Cdc42 signaling pathways important for actin reorganization. Within these pathways it interacts with proteins such as Rho family GTPases influencing cytoskeletal arrangements. The proper functioning of these pathways is vital for numerous cellular processes including the migration of cells which affects tissue development and repair.

Alterations in N-WASP are linked to immunodeficiencies and neurological disorders. Mutations or dysfunction in N-WASP can lead to Wiskott-Aldrich syndrome characterized by immune defects and eczema due to its role in immune cell signaling. Additionally N-WASP's interaction with proteins such as WASP contributes to its involvement in neurodevelopmental disorders like autistic spectrum disorders reflecting the importance of this protein in maintaining normal cellular function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex (PubMed : 16767080, PubMed : 19366662, PubMed : 19487689, PubMed : 22847007, PubMed : 22921828, PubMed : 9422512). Involved in various processes, such as mitosis and cytokinesis, via its role in the regulation of actin polymerization (PubMed : 19366662, PubMed : 19487689, PubMed : 22847007, PubMed : 22921828, PubMed : 9422512). Together with CDC42, involved in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia (PubMed : 9422512). In addition to its role in the cytoplasm, also plays a role in the nucleus by regulating gene transcription, probably by promoting nuclear actin polymerization (PubMed : 16767080). Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression (By similarity). Plays a role in dendrite spine morphogenesis (By similarity). Decreasing levels of DNMBP (using antisense RNA) alters apical junction morphology in cultured enterocytes, junctions curve instead of being nearly linear (PubMed : 19767742).
See full target information Neural Wiskott-Aldrich syndrome protein phospho Y256
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 11:118 PubMed32051399

2020

Targeting Src family kinase member Fyn by Saracatinib attenuated liver fibrosis in vitro and in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Guifang Du,Jing Wang,Ting Zhang,Qiang Ding,Xiaodong Jia,Xueke Zhao,Jinke Dong,Xinrui Yang,Shanshan Lu,Cuihong Zhang,Ze Liu,Zhen Zeng,Rifaat Safadi,Ruizhao Qi,Xin Zhao,Zhixian Hong,Yinying Lu

The Journal of physiology 596:3617-3635 PubMed29746010

2018

Rho kinase collaborates with p21-activated kinase to regulate actin polymerization and contraction in airway smooth muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Wenwu Zhang,Bhupal P Bhetwal,Susan J Gunst

The Journal of physiology 594:4879-900 PubMed27038336

2016

p21-Activated kinase (Pak) regulates airway smooth muscle contraction by regulating paxillin complexes that mediate actin polymerization.

Applications

Unspecified application

Species

Unspecified reactive species

Wenwu Zhang,Youliang Huang,Susan J Gunst

The Journal of biological chemistry 288:4241-50 PubMed23212915

2012

Neural Wiskott-Aldrich syndrome protein (N-WASP)-mediated p120-catenin interaction with Arp2-Actin complex stabilizes endothelial adherens junctions.

Applications

WB

Species

Human

Charu Rajput,Vidisha Kini,Monica Smith,Pascal Yazbeck,Alejandra Chavez,Tracy Schmidt,Wei Zhang,Nebojsa Knezevic,Yulia Komarova,Dolly Mehta

The Journal of biological chemistry 287:33996-4008 PubMed22893699

2012

The small GTPase RhoA regulates the contraction of smooth muscle tissues by catalyzing the assembly of cytoskeletal signaling complexes at membrane adhesion sites.

Applications

WB

Species

Dog

Wenwu Zhang,Youliang Huang,Susan J Gunst

Development (Cambridge, England) 139:3200-10 PubMed22872087

2012

Focal adhesion kinase regulates actin nucleation and neuronal filopodia formation during axonal growth.

Applications

WB

Species

Unspecified reactive species

Mariola R Chacón,Ana I Navarro,German Cuesto,Isabel del Pino,Ricardo Scott,Miguel Morales,Beatriz Rico
View all publications
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