AB211488

Anti-N2,N2-dimethylguanosine (m2,2G) antibody [EPR-19838-40]

20ul selling size
RabMAb
Recombinant
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(3 Publications)

Rabbit Recombinant Monoclonal N2,N2-dimethylguanosine (m2,2G) antibody. Suitable for Dot, IP, ELISA and reacts with Modified Nucleic Acid samples. Cited in 3 publications.

View Alternative Names

Di-m2G, N2,N2-dimethylguanosine, m22G

4 Images

Supplier Data

Immunoprecipitation - Anti-N2,N2-dimethylguanosine (m2,2G) antibody [EPR-19838-40] (AB211488)

The IP was performed in a U-bottom non-adsorbing propylene 96-well plate.

ab211488 (0.2 μg) was coated into Dynabeads^® sheep-anti-rabbit IgG (50 μl) for 1h at RT.

Unmodified/modified oligonucleotides (5 μM) were added to samples containing the antibody/bead complexes and incubated with agitation for 1 hour at RT.

After washing, Peroxidase-conjugated Streptavidin was incubated at 1/1000 dilution with agitation for 1 hour at RT.

ECL substrate was then added and the results read in a non-transparent 96-well plate with a digital detector and analyzed using ImageJ.

Lane 1 : Buffer only.

Lane 2 : Modified oligonucleotide (5 μM), 5' Biotin-mN.mN.mN.mN.mN.[m2,2G]*.mN.mN.mN.mN.mN 3'

Lane 3 : Unmodified oligonucleotide (5 μM), 5' Biotin-mN.mN.mN.mN.mN.[G]*.mN.mN.mN.mN.mN 3'

N - equimolar mixture of (A/U/G/C)
m - 2'O methyl protection
* - phosphorothioate protection

Blocking buffer and concentration : 5% NFDM/TBST

Dilution buffer and concentration : TBST/0.1% Triton X-100/1 mM EDTA

All lanes:

Immunoprecipitation - Anti-N2,N2-dimethylguanosine (m2,2G) antibody [EPR-19838-40] (ab211488)

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ELISA - Anti-N2,N2-dimethylguanosine (m2,2G) antibody [EPR-19838-40] (AB211488)

ELISA

Supplier Data

ELISA - Anti-N2,N2-dimethylguanosine (m2,2G) antibody [EPR-19838-40] (AB211488)

BSA-conjugated m22G (modified) and G (unmodified) nucleosides were coated onto wells of a 96 well plate. ELISA was performed on 1.0 μg/ml of antigen using ab211488 at a concentration range of 0.005-4.000 μg/ml followed by Goat Anti-Rabbit IgG (H+L) alkaline phosphatase conjugated secondary antibody at 1/2500 dilution.

Lab

Dot Blot - Anti-N2,N2-dimethylguanosine (m2,2G) antibody [EPR-19838-40] (AB211488)

Primary antibody dilution : 1/500

Secondary antibody : Goat Anti-Rabbit IgG (H+L) Peroxidase conjugated

Secondary antibody dilution : 1/100000

Blocking and dilution buffer : AdvanBlockTM Chemi Blocking buffer

Input : Biotin-m2.2G oligo 2 ng per Dot

Competitive nucleosides : m2.2G m2G m1G

Exposure times : 37 seconds

Lab

Dot Blot - Anti-N2,N2-dimethylguanosine (m2,2G) antibody [EPR-19838-40] (AB211488)

Lane 1 : Yeast tRNA

Lane 2 : Biotin-m2.2G RNA oligo

Lane 3 : Biotin-G RNA oligo

Primary antibody dilution : 1/500

Secondary antibody : Goat Anti-Rabbit IgG (H+L) Peroxidase conjugated

Secondary antibody dilution : 1/20000

Blocking and dilution buffer : AdvanBlockTM Chemi Blocking buffer

Carrier free

Anti-N2,N2-dimethylguanosine (m2,2G) antibody [EPR-19838-40] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR-19838-40

Isotype

IgG

Carrier free

Applications

IP, Dot, ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Has been developed to discriminate between the modified base N2,N2-dimethylguanosine (m2,2G) and the unmodified counterpart Guanosine (G).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Modified Nucleic Acid": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "0.2 μg coated onto 50 μL Dynabeads® sheep-anti-rabbit IgG.", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "0.005-4 µg/mL", "ELISA-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

N2N2-dimethylguanosine (m22G) is a modified nucleoside found in various types of RNA including tRNA and rRNA. It plays a role in the cellular machinery by contributing to the structural stability and function of RNA molecules. Its mass is approximately 297 g/mol. Expression of m22G occurs in the cellular RNA of many organisms reflecting its essential role in RNA structure. The modification m22G often referred to as a methylated derivative of guanosine is implicated in numerous cellular processes due to its presence in key RNA components.

Biological function summary

The modification of guanine at the N2 position influences RNA stability and function by enhancing the efficiency of translation. It forms part of the ribosome's active sites appearing as part of larger ribonucleoprotein complexes which are important for carrying out protein synthesis. This modification helps maintain the correct structure of ribosomal RNA ensuring accurate and efficient protein production. The presence of m22G in RNA components aids in the prevention of frameshift mutations during translation marking its importance in maintaining genetic fidelity.

Pathways

M22G plays a significant role in the translation and protein synthesis pathway contributing to the proper assembly and function of ribosomes. The presence of m22G within these pathways ensures the proper execution of genetic code translation interacting closely with proteins like ribosomal protein S15 and other translational machinery components. These interactions are essential in coordinating the synthesis of proteins impacting cellular growth and development functions.

M22G has connections with certain cancers and neurological disorders due to its involvement in regulating RNA stability and translation efficiency. Alterations or dysregulation in the patterns of m22G methylation can contribute to carcinogenesis by affecting the accuracy of protein translation. Proteins involved in this dysregulation include ribosomal proteins and RNA methyltransferases which can lead to the development of malignant transformation. Additionally insufficient or aberrant methylation of guanosine can contribute to neurodegenerative diseases like Alzheimer's where impaired RNA function results from transcriptional and translational inefficiencies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information N2,N2-dimethylguanosine (m2,2G)

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

STAR protocols 6:103924 PubMed40601427

2025

Protocol to detect mG modification on single tRNA iso-decoders by immuno-northern blotting.

Applications

Unspecified application

Species

Unspecified reactive species

Sseu-Pei Hwang,Katherine Barondeau,Catherine Denicourt

ACS chemical biology 17:2789-2800 PubMed36190452

2022

Conditional Covalent Lethality Driven by Oncometabolite Accumulation.

Applications

Unspecified application

Species

Unspecified reactive species

Minervo Perez,Kellie D Nance,Daniel W Bak,Supuni Thalalla Gamage,Susana S Najera,Amy N Conte,W Marston Linehan,Eranthie Weerapana,Jordan L Meier

Scientific reports 7:11383 PubMed28900184

2017

Survivin and XIAP - two potential biological targets in follicular thyroid carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas A Werner,Levent Dizdar,Inga Nolten,Jasmin C Riemer,Sabrina Mersch,Sina C Schütte,Christiane Driemel,Pablo E Verde,Katharina Raba,Stefan A Topp,Matthias Schott,Wolfram T Knoefel,Andreas Krieg

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-N2,N2-dimethylguanosine (m2,2G) antibody [EPR-19838-40]

Immunoprecipitation - Anti-N2,N2-dimethylguanosine (m2,2G) antibody [EPR-19838-40] (AB211488)

ELISA - Anti-N2,N2-dimethylguanosine (m2,2G) antibody [EPR-19838-40] (AB211488)

Dot Blot - Anti-N2,N2-dimethylguanosine (m2,2G) antibody [EPR-19838-40] (AB211488)

Dot Blot - Anti-N2,N2-dimethylguanosine (m2,2G) antibody [EPR-19838-40] (AB211488)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Applications

Immunogen

Specificity

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise