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Rabbit Recombinant Monoclonal N2,N2-dimethylguanosine (m2,2G) antibody. Carrier free. Suitable for IP, ELISA, Dot and reacts with Modified Nucleic Acid samples.

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Images

Dot Blot - Anti-N2,N2-dimethylguanosine (m2,2G) antibody [EPR-19838-40] - BSA and Azide free (AB251526), expandable thumbnail
  • Immunoprecipitation - Anti-N2,N2-dimethylguanosine (m2,2G) antibody [EPR-19838-40] - BSA and Azide free (AB251526), expandable thumbnail
  • Dot Blot - Anti-N2,N2-dimethylguanosine (m2,2G) antibody [EPR-19838-40] - BSA and Azide free (AB251526), expandable thumbnail
  • ELISA - Anti-N2,N2-dimethylguanosine (m2,2G) antibody [EPR-19838-40] - BSA and Azide free (AB251526), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPELISADot
Modified Nucleic Acid
Tested
Tested
Tested

Tested
Tested

Species
Modified Nucleic Acid
Dilution info
-
Notes

-

Tested
Tested

Species
Modified Nucleic Acid
Dilution info
-
Notes

-

Tested
Tested

Species
Modified Nucleic Acid
Dilution info
-
Notes

-

Target data

Alternative names

Di-m2G, N2,N2-dimethylguanosine, m22G

Recommended products

Rabbit Recombinant Monoclonal N2,N2-dimethylguanosine (m2,2G) antibody. Carrier free. Suitable for IP, ELISA, Dot and reacts with Modified Nucleic Acid samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR-19838-40
Purification technique
Affinity purification Protein A
Specificity

Has been developed to discriminate between the modified base N2,N2-dimethylguanosine (m2,2G) and the unmodified counterpart Guanosine (G).

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab251526 is the carrier-free version of Anti-N2,N2-dimethylguanosine (m2,2G) antibody [EPR-19838-40] ab211488.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

N2N2-dimethylguanosine (m22G) is a modified nucleoside found in various types of RNA including tRNA and rRNA. It plays a role in the cellular machinery by contributing to the structural stability and function of RNA molecules. Its mass is approximately 297 g/mol. Expression of m22G occurs in the cellular RNA of many organisms reflecting its essential role in RNA structure. The modification m22G often referred to as a methylated derivative of guanosine is implicated in numerous cellular processes due to its presence in key RNA components.

Biological function summary

The modification of guanine at the N2 position influences RNA stability and function by enhancing the efficiency of translation. It forms part of the ribosome's active sites appearing as part of larger ribonucleoprotein complexes which are important for carrying out protein synthesis. This modification helps maintain the correct structure of ribosomal RNA ensuring accurate and efficient protein production. The presence of m22G in RNA components aids in the prevention of frameshift mutations during translation marking its importance in maintaining genetic fidelity.

Pathways

M22G plays a significant role in the translation and protein synthesis pathway contributing to the proper assembly and function of ribosomes. The presence of m22G within these pathways ensures the proper execution of genetic code translation interacting closely with proteins like ribosomal protein S15 and other translational machinery components. These interactions are essential in coordinating the synthesis of proteins impacting cellular growth and development functions.

Associated diseases and disorders

M22G has connections with certain cancers and neurological disorders due to its involvement in regulating RNA stability and translation efficiency. Alterations or dysregulation in the patterns of m22G methylation can contribute to carcinogenesis by affecting the accuracy of protein translation. Proteins involved in this dysregulation include ribosomal proteins and RNA methyltransferases which can lead to the development of malignant transformation. Additionally insufficient or aberrant methylation of guanosine can contribute to neurodegenerative diseases like Alzheimer's where impaired RNA function results from transcriptional and translational inefficiencies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Dot Blot - Anti-N2,N2-dimethylguanosine (m2,2G) antibody [EPR-19838-40] - BSA and Azide free (ab251526), expandable thumbnail

    Dot Blot - Anti-N2,N2-dimethylguanosine (m2,2G) antibody [EPR-19838-40] - BSA and Azide free (ab251526)

    This data was developed using Anti-N2,N2-dimethylguanosine (m2,2G) antibody [EPR-19838-40] ab211488, the same antibody clone in a different buffer formulation.Primary antibody dilution: 1/500 Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated Secondary antibody dilution: 1/100,000 Blocking and dilution buffer: AdvanBlockTM Chemi Blocking buffer Input: Biotin-m2.2G oligo 2 ng per Dot Competitive nucleosides: m2.2G, m2G, m1G Exposure times: 37 seconds

  • Immunoprecipitation - Anti-N2,N2-dimethylguanosine (m2,2G) antibody [EPR-19838-40] - BSA and Azide free (ab251526), expandable thumbnail

    Immunoprecipitation - Anti-N2,N2-dimethylguanosine (m2,2G) antibody [EPR-19838-40] - BSA and Azide free (ab251526)

    This data was developed using Anti-N2,N2-dimethylguanosine (m2,2G) antibody [EPR-19838-40] ab211488, the same antibody clone in a different buffer formulation.

    The IP was performed in a U-bottom non-adsorbing propylene 96-well plate.

    Anti-N2,N2-dimethylguanosine (m2,2G) antibody [EPR-19838-40] ab211488 (0.2 μg) was coated into Dynabeads® sheep-anti-rabbit IgG (50 μl) for 1h at RT.

    Unmodified/modified oligonucleotides (5 μM) were added to samples containing the antibody/bead complexes and incubated with agitation for 1 hour at RT.

    After washing, Peroxidase-conjugated Streptavidin was incubated at 1/1000 dilution with agitation for 1 hour at RT.

    ECL substrate was then added and the results read in a non-transparent 96-well plate with a digital detector and analyzed using ImageJ.

    Lane 1: Buffer only.

    Lane 2: Modified oligonucleotide (5 μM), 5' Biotin-mN.mN.mN.mN.mN.[m2,2G]*.mN.mN.mN.mN.mN 3'

    Lane 3: Unmodified oligonucleotide (5 μM), 5' Biotin-mN.mN.mN.mN.mN.[G]*.mN.mN.mN.mN.mN 3'

    N - equimolar mixture of (A/U/G/C)
    m - 2'O methyl protection
    * - phosphorothioate protection

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: TBST/0.1% Triton X-100/1 mM EDTA.

    All lanes: Immunoprecipitation - Anti-N2,N2-dimethylguanosine (m2,2G) antibody [EPR-19838-40] (Anti-N2,N2-dimethylguanosine (m2,2G) antibody [EPR-19838-40] ab211488)

    Developed using the ECL technique.

  • Dot Blot - Anti-N2,N2-dimethylguanosine (m2,2G) antibody [EPR-19838-40] - BSA and Azide free (ab251526), expandable thumbnail

    Dot Blot - Anti-N2,N2-dimethylguanosine (m2,2G) antibody [EPR-19838-40] - BSA and Azide free (ab251526)

    This data was developed using Anti-N2,N2-dimethylguanosine (m2,2G) antibody [EPR-19838-40] ab211488, the same antibody clone in a different buffer formulation.Lane 1: Yeast tRNA Lane 2: Biotin-m2.2G RNA oligo Lane 3: Biotin-G RNA oligo Primary antibody dilution: 1/500 Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated Secondary antibody dilution: 1/20,000 Blocking and dilution buffer: AdvanBlockTM Chemi Blocking buffer

  • ELISA - Anti-N2,N2-dimethylguanosine (m2,2G) antibody [EPR-19838-40] - BSA and Azide free (ab251526), expandable thumbnail

    ELISA - Anti-N2,N2-dimethylguanosine (m2,2G) antibody [EPR-19838-40] - BSA and Azide free (ab251526)

    This data was developed using Anti-N2,N2-dimethylguanosine (m2,2G) antibody [EPR-19838-40] ab211488, the same antibody clone in a different buffer formulation.BSA-conjugated m2,2G (modified) and G (unmodified) nucleosides were coated onto wells of a 96 well plate. ELISA was performed on 1.0 µg/ml of antigen using Anti-N2,N2-dimethylguanosine (m2,2G) antibody [EPR-19838-40] ab211488 at a concentration range of 0.005-4.000 µg/ml, followed by Goat Anti-Rabbit IgG, (H+L), alkaline phosphatase conjugated secondary antibody at 1/2500 dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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