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AB252215

Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128]

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(27 Publications)

Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] (ab252215) is a rabbit monoclonal antibody detecting N4-acetylcytidine (ac4C) in Dot Blot.

- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications
8 Images
Dot Blot - Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] (AB252215)
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Lab

Dot Blot - Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] (AB252215)

Dot blot analysis of N4-acetylcytidine (ac4C) labeled with ab252215 at 1/200 dilution.

Lane 1 : HeLa total RNA
Lane 2 : ac4C RNA oligo
Lane 3 : C RNA oligo

Goat Anti-Rabbit IgG H&L (HRP) Peroxidase conjugated (ab97051) at 1/20,000 dilution was used as secondary antibody.

Ac4C oligo (without biotin labelled) used in the figure was provided by a collaborator.

Dot Blot - Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] (AB252215)
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PubMed

Dot Blot - Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] (AB252215)

Dot blot analysis showing effect of hydroxylamine on ac4C in RNA using anti-ac4C antibody. Reaction conditions : 50 mM hydroxylamine, Tris at pH 8.0, 65 °C, 1 hour. Hydroxylamine reduced the detection of ac4C.

Refer to PMID 29039931

Image from Sinclair WR et al., ACS Chemical Biology., 12 (12), 2922-2926. Fig 4C.; doi : 10.1021/acschembio.7b00734.

Image from Sinclair WR et al., ACS Chemical Biology., 12 (12), 2922-2926. Fig 4C.; doi: 10.1021/acschembio.7b00734.

Dot Blot - Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] (AB252215)
  • Dot

PubMed

Dot Blot - Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] (AB252215)

Dot blot analysis of total RNA by anti-ac4C found a significantly decreased signal in hydroxylamine-treated samples compared to untreated samples.

Refer to PMID 29039931

<https : //www.ncbi.nlm.nih.gov/pubmed/?term=29039931>.

Image from Sinclair WR et al., ACS Chemical Biology., 12 (12), 2922-2926. Fig 4F.; doi : 10.1021/acschembio.7b00734.

Image from Sinclair WR et al., ACS Chemical Biology., 12 (12), 2922-2926. Fig 4F.; doi: 10.1021/acschembio.7b00734.

Dot Blot - Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] (AB252215)
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Unknown

Dot Blot - Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] (AB252215)

Competitive RNA Dot Blot - Recombinant Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] (ab252215)

Primary antibody dilution : 1 ug/ml

Secondary antibody : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated

Secondary antibody dilution : 1/20,000

Blocking buffer and dilution buffer : AdvanBlock™ Chemi Blocking buffer

Input : HeLa total RNA 500ng per Dot

Competitive nucleosides :

Lane A : ac4C

Lane B : unmodified C

Exposure time : 137 seconds

This blot was developed using a higher sensitivity ECL substrate

Dot Blot - Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] (AB252215)
  • Dot

Unknown

Dot Blot - Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] (AB252215)

Dot blot analysis of N4-acetylcytidine (ac4C) labeled with ab252215 at 1/1000 dilution.

Lane 1 : Synthetic wild type Beta globin RNA probe.
Lane 2 : Synthetic Beta globin RNA probe containing ac4C.
Lane 3 : Synthetic Beta globin RNA probe containing m5C.
Lane 4 : Beta globin RNA probe containing hm5C.

Goat Anti-Rabbit IgG H&L (HRP) at 1/100000 dilution was used as secondary antibody.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 2 seconds.

This antibody shows no cross reactivity with other RNA modifications.

This image was kindly provided by Dr. Jordan Meier, NCI, USA.

This antibody was cited in PMID : 29039931 and 30449621.

Dot Blot - Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] (AB252215)
  • Dot

PubMed

Dot Blot - Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] (AB252215)

Dot blot analysis demonstrates concentration-dependent detection of in vitro transcribed ac4C-containing RNAs. The antibody detects RNA probes synthesized in the presence of ac4CTP, but not CTP.

Refer to PMID 29039931

Image from Sinclair WR et al., ACS Chemical Biology., 12 (12), 2922-2926. Fig 3C.; doi : 10.1021/acschembio.7b00734.

Image from Sinclair WR et al., ACS Chemical Biology., 12 (12), 2922-2926. Fig 3C.; doi: 10.1021/acschembio.7b00734.

Dot Blot - Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] (AB252215)
  • Dot

Unknown

Dot Blot - Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] (AB252215)

Dot blot analysis of N4-acetylcytidine (ac4C) labeled with ab252215 at 1/1000 dilution.

Lane 1 : Synthetic wild type E. coli tRNA Met probe.
Lane 2 : Synthetic E. coli tRNA Met probe containing ac4C.
Lane 3 : Synthetic E. coli tRNA Met probe containing ac4C pre-treated with 50mM hydroxylamine.

Goat Anti-Rabbit IgG H&L (HRP) at 1/100000 dilution was used as the secondary antibody.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 2 seconds.

Hydroxylamine removes the electrophilic acetamide of ac4C.

This image was kindly provided by Dr. Jordan Meier, NCI, USA.

This antibody was cited in PMID : 29039931 and 30449621.

Dot Blot - Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] (AB252215)
  • Dot

Unknown

Dot Blot - Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] (AB252215)

Dot blot analysis of N4-acetylcytidine (ac4C) labeled with either ab252215 at 1/1000 dilution or with methylene blue.

Lane 1 : Total RNA purified from wild-type HeLa cells.
Lane 2 : Total RNA purified from NAT10 knockout HeLa cells

Goat Anti-Rabbit IgG H&L (HRP) at 1/100000 dilution was used as secondary antibody.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times : Left panel, 2 minutes. Right panel, 30 seconds.

Knock out of NAT10 (human acetyltransferase) can dramatically reduce ac4C level.

This image was kindly provided by Dr. Jordan Meier, NCI, USA.

This antibody was cited in PMID : 29039931 and 30449621.

  • Carrier free

    Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPRNCI-184-128

Isotype

IgG

Carrier free

No

Applications

Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Modified Nucleic Acid": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/200 - 1/1000", "Dot-species-notes": "<p></p>" } } }
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Product details

What is this antibody validated in?
Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] (ab252215) is a rabbit recombinant monoclonal antibody and is validated for use in Dot Blot. Trusted by the scientific community
Anti-N4-acetylcytidine (ac4C) [EPRNCI-184-128] (ab252215) was first used in a scientific publication in 2019 and has been cited over 10 times in peer-reviewed journals.

Other related products
We have a range of other formats of antibody clone [EPRNCI-184-128] also available for your convenience: ab252215, Carrier free - ab253039

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

N4-acetylcytidine (ac4C) a modified nucleoside occurs in various types of RNA like tRNA rRNA and mRNA. The molecular mass of ac4C roughly totals 255 daltons. It is expressed ubiquitously across many organisms including humans yeast and bacteria. This modification also referred to sometimes as an 'RNA acetylation mark' contributes to the stability and codon-anticodon interactions in tRNA with an important role in maintaining the structural integrity of rRNA components.
Biological function summary

N4-acetylcytidine acts to enhance translation fidelity which is essential for accurate gene expression. It ensures the stabilization of RNA structures and impacts the efficiency of translation. This modification often involves an enzyme complex including acetyltransferases which work by transferring the acetyl group from acetyl-CoA to the cytidine residue. Proper functioning and balance of ac4C modifications in RNA depend on these enzymes ensuring that the RNA molecule remains functional and effective in protein synthesis.

Pathways

N4-acetylcytidine takes part in translation and RNA processing pathways. It works alongside RNA polymerase complexes and other enzymes that modify RNA. The modification of ac4C enhances the initiation and elongation phases of translation. Within these pathways it interacts with proteins such as RNA acetylating enzymes and ribosomal proteins to fine-tune these processes ensuring that RNA synthesis and subsequent protein production are precise and efficient.

N4-acetylcytidine shows links to conditions related to translation errors and cellular stress responses. Aberrant levels of ac4C have connections to cancer wherein dysregulated translation and misfolded protein formation occur. Defective ac4C modification might lead to neurodegeneration where accurate protein synthesis is compromised. Proteins like NAT10 a known RNA acetyltransferase interact with ac4C in these pathological contexts indicating the importance of maintaining balanced ac4C levels for cellular health and disease prevention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information N4-acetylcytidine (ac4C)
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Publications (27)

Recent publications for all applications. Explore the full list and refine your search

Clinics (Sao Paulo, Brazil) 80:100658 PubMed40245789

2025

Kynurenine promotes the immune escape of colorectal cancer cells via NAT10-mediated acC acetylation of PD-L1.

Applications

Unspecified application

Species

Unspecified reactive species

Zaibiao Wang,Manman Yin,Ruhang Zhou,Ming Li,Jie Peng,Zhengguang Wang

CytoJournal 21:68 PubMed39917001

2025

N-acetyltransferase 10 regulates UNC-51-like kinase 1 to reduce tubular cell injury and kidney stone formation.

Applications

Unspecified application

Species

Unspecified reactive species

Le Wang,Jinjing Huang,Lei Song,Ben Ke

Cell reports. Medicine 5:101728 PubMed39293390

2024

Targeting NAT10 inhibits osteosarcoma progression via ATF4/ASNS-mediated asparagine biosynthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yutong Zou,Siyao Guo,Lili Wen,Dongming Lv,Jian Tu,Yan Liao,Weidong Chen,Ziyun Chen,Hongbo Li,Junkai Chen,Jingnan Shen,Xianbiao Xie

British journal of cancer 130:201-212 PubMed38040817

2023

Downregulation of N4-acetylcytidine modification in myeloid cells attenuates immunotherapy and exacerbates hepatocellular carcinoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Xu,Jianyong Zhuo,Yiyuan Chen,Renyi Su,Huan Chen,Zhensheng Zhang,Zhengxing Lian,Di Lu,Xuyong Wei,Shusen Zheng,Xiao Xu,Shuai Wang,Qiang Wei

Nature communications 14:6327 PubMed37816771

2023

NAT10-dependent N-acetylcytidine modification mediates PAN RNA stability, KSHV reactivation, and IFI16-related inflammasome activation.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Yan,Jing Zhou,Ziyu Wang,Xiangya Ding,Xinyue Ma,Wan Li,Xuemei Jia,Shou-Jiang Gao,Chun Lu

International journal of medical sciences 20:1079-1090 PubMed37484809

2023

NAT10-mediated RNA acetylation enhances HNRNPUL1 mRNA stability to contribute cervical cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Yingfei Long,Yifei Ren,Qinglv Wei,Youchaou Mobet,Yujiao Liu,Hongyan Zhao,Tao Liu,Lei Cheng,Ping Yi

Nature communications 14:3729 PubMed37349316

2023

Maternal NAT10 orchestrates oocyte meiotic cell-cycle progression and maturation in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Jiang,Yu Cheng,Yuzhang Zhu,Caoling Xu,Qiaodan Li,Xuemei Xing,Wenqing Li,Jiaqi Zou,Lan Meng,Muhammad Azhar,Yuzhu Cao,Xianhong Tong,Weibing Qin,Xiaoli Zhu,Jianqiang Bao

Epigenetics & chromatin 16:26 PubMed37322549

2023

PARP-dependent and NAT10-independent acetylation of N4-cytidine in RNA appears in UV-damaged chromatin.

Applications

Unspecified application

Species

Unspecified reactive species

Alena Svobodová Kovaříková,Lenka Stixová,Aleš Kovařík,Eva Bártová

Epigenetics 18:2188667 PubMed36908042

2023

NAT10 mediated mRNA acetylation modification patterns associated with colon cancer progression and microsatellite status.

Applications

Unspecified application

Species

Unspecified reactive species

Hailin Zhang,Wenqing Shan,Zhenwei Yang,Yangyang Zhang,Meng Wang,Liping Gao,Lingxiu Zeng,Qiu Zhao,Jing Liu

The Journal of neuroscience : the official journal of the Society for Neuroscience 43:3009-3027 PubMed36898834

2023

The Cytidine N-Acetyltransferase NAT10 Participates in Peripheral Nerve Injury-Induced Neuropathic Pain by Stabilizing SYT9 Expression in Primary Sensory Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Zhang,Kehui Yang,Qi-Hui Wang,Ling Xie,Qiaoqiao Liu,Runa Wei,Yang Tao,Hong-Li Zheng,Ninghua Lin,Hengjun Xu,Li Yang,Hongjun Wang,Tingruo Zhang,Zhouya Xue,Jun-Li Cao,Zhiqiang Pan
View all publications
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