AB252215

Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128]

RabMAb
Recombinant
20ul selling size
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(68 Publications )

Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] (ab252215) is a rabbit monoclonal antibody detecting N4-acetylcytidine (ac4C) in Dot Blot.

- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications

8 Images

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Dot Blot - Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] (AB252215)

Competitive RNA Dot Blot - Recombinant Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] (ab252215)

Primary antibody dilution : 1 ug/ml

Secondary antibody : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated

Secondary antibody dilution : 1/20,000

Blocking buffer and dilution buffer : AdvanBlock™ Chemi Blocking buffer

Input : HeLa total RNA 500ng per Dot

Competitive nucleosides :

Lane A : ac4C

Lane B : unmodified C

Exposure time : 137 seconds

This blot was developed using a higher sensitivity ECL substrate

Lab

Dot Blot - Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] (AB252215)

Dot blot analysis of N4-acetylcytidine (ac4C) labeled with ab252215 at 1/200 dilution.

Lane 1 : HeLa total RNA
Lane 2 : ac4C RNA oligo
Lane 3 : C RNA oligo

Goat Anti-Rabbit IgG H&L (HRP) Peroxidase conjugated (ab97051) at 1/20,000 dilution was used as secondary antibody.

Ac4C oligo (without biotin labelled) used in the figure was provided by a collaborator.

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Dot Blot - Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] (AB252215)

Dot blot analysis of N4-acetylcytidine (ac4C) labeled with either ab252215 at 1/1000 dilution or with methylene blue.

Lane 1 : Total RNA purified from wild-type HeLa cells.
Lane 2 : Total RNA purified from NAT10 knockout HeLa cells

Goat Anti-Rabbit IgG H&L (HRP) at 1/100000 dilution was used as secondary antibody.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times : Left panel, 2 minutes. Right panel, 30 seconds.

Knock out of NAT10 (human acetyltransferase) can dramatically reduce ac4C level.

This image was kindly provided by Dr. Jordan Meier, NCI, USA.

This antibody was cited in PMID : 29039931 and 30449621.

PubMed

Dot Blot - Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] (AB252215)

Dot blot analysis showing effect of hydroxylamine on ac4C in RNA using anti-ac4C antibody. Reaction conditions : 50 mM hydroxylamine, Tris at pH 8.0, 65 °C, 1 hour. Hydroxylamine reduced the detection of ac4C.

Refer to PMID 29039931

Image from Sinclair WR et al., ACS Chemical Biology., 12 (12), 2922-2926. Fig 4C.; doi : 10.1021/acschembio.7b00734.

Image from Sinclair WR et al., ACS Chemical Biology., 12 (12), 2922-2926. Fig 4C.; doi: 10.1021/acschembio.7b00734.

PubMed

Dot Blot - Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] (AB252215)

Dot blot analysis of total RNA by anti-ac4C found a significantly decreased signal in hydroxylamine-treated samples compared to untreated samples.

Refer to PMID 29039931

<https://www.ncbi.nlm.nih.gov/pubmed/?term=29039931>.

Image from Sinclair WR et al., ACS Chemical Biology., 12 (12), 2922-2926. Fig 4F.; doi : 10.1021/acschembio.7b00734.

Image from Sinclair WR et al., ACS Chemical Biology., 12 (12), 2922-2926. Fig 4F.; doi: 10.1021/acschembio.7b00734.

Unknown

Dot Blot - Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] (AB252215)

Dot blot analysis of N4-acetylcytidine (ac4C) labeled with ab252215 at 1/1000 dilution.

Lane 1 : Synthetic wild type Beta globin RNA probe.
Lane 2 : Synthetic Beta globin RNA probe containing ac4C.
Lane 3 : Synthetic Beta globin RNA probe containing m5C.
Lane 4 : Beta globin RNA probe containing hm5C.

Goat Anti-Rabbit IgG H&L (HRP) at 1/100000 dilution was used as secondary antibody.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 2 seconds.

This antibody shows no cross reactivity with other RNA modifications.

This image was kindly provided by Dr. Jordan Meier, NCI, USA.

This antibody was cited in PMID : 29039931 and 30449621.

PubMed

Dot Blot - Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] (AB252215)

Dot blot analysis demonstrates concentration-dependent detection of in vitro transcribed ac4C-containing RNAs. The antibody detects RNA probes synthesized in the presence of ac4CTP, but not CTP.

Refer to PMID 29039931

Image from Sinclair WR et al., ACS Chemical Biology., 12 (12), 2922-2926. Fig 3C.; doi : 10.1021/acschembio.7b00734.

Image from Sinclair WR et al., ACS Chemical Biology., 12 (12), 2922-2926. Fig 3C.; doi: 10.1021/acschembio.7b00734.

Unknown

Dot Blot - Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] (AB252215)

Dot blot analysis of N4-acetylcytidine (ac4C) labeled with ab252215 at 1/1000 dilution.

Lane 1 : Synthetic wild type E. coli tRNA Met probe.
Lane 2 : Synthetic E. coli tRNA Met probe containing ac4C.
Lane 3 : Synthetic E. coli tRNA Met probe containing ac4C pre-treated with 50mM hydroxylamine.

Goat Anti-Rabbit IgG H&L (HRP) at 1/100000 dilution was used as the secondary antibody.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 2 seconds.

Hydroxylamine removes the electrophilic acetamide of ac4C.

This image was kindly provided by Dr. Jordan Meier, NCI, USA.

This antibody was cited in PMID : 29039931 and 30449621.

Carrier free

Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPRNCI-184-128

Isotype

IgG

Carrier free

Applications

Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Modified Nucleic Acid": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/200 - 1/1000", "Dot-species-notes": "<p></p>" } } }

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Product details

What is this antibody validated in?
Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] (ab252215) is a rabbit recombinant monoclonal antibody and is validated for use in Dot Blot. Trusted by the scientific community
Anti-N4-acetylcytidine (ac4C) [EPRNCI-184-128] (ab252215) was first used in a scientific publication in 2019 and has been cited over 10 times in peer-reviewed journals.

Other related products
We have a range of other formats of antibody clone [EPRNCI-184-128] also available for your convenience: ab252215, Carrier free - ab253039

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

N4-acetylcytidine (ac4C) a modified nucleoside occurs in various types of RNA like tRNA rRNA and mRNA. The molecular mass of ac4C roughly totals 255 daltons. It is expressed ubiquitously across many organisms including humans yeast and bacteria. This modification also referred to sometimes as an 'RNA acetylation mark' contributes to the stability and codon-anticodon interactions in tRNA with an important role in maintaining the structural integrity of rRNA components.

Biological function summary

N4-acetylcytidine acts to enhance translation fidelity which is essential for accurate gene expression. It ensures the stabilization of RNA structures and impacts the efficiency of translation. This modification often involves an enzyme complex including acetyltransferases which work by transferring the acetyl group from acetyl-CoA to the cytidine residue. Proper functioning and balance of ac4C modifications in RNA depend on these enzymes ensuring that the RNA molecule remains functional and effective in protein synthesis.

Pathways

N4-acetylcytidine takes part in translation and RNA processing pathways. It works alongside RNA polymerase complexes and other enzymes that modify RNA. The modification of ac4C enhances the initiation and elongation phases of translation. Within these pathways it interacts with proteins such as RNA acetylating enzymes and ribosomal proteins to fine-tune these processes ensuring that RNA synthesis and subsequent protein production are precise and efficient.

N4-acetylcytidine shows links to conditions related to translation errors and cellular stress responses. Aberrant levels of ac4C have connections to cancer wherein dysregulated translation and misfolded protein formation occur. Defective ac4C modification might lead to neurodegeneration where accurate protein synthesis is compromised. Proteins like NAT10 a known RNA acetyltransferase interact with ac4C in these pathological contexts indicating the importance of maintaining balanced ac4C levels for cellular health and disease prevention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information N4-acetylcytidine (ac4C)

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Publications (68)

Recent publications for all applications. Explore the full list and refine your search

Cell biology and toxicology 41:125 PubMed40794120

2025

NAT10 Mediates Cardiac Fibrosis Induced by Myocardial Infarction Through ac4C Modification of TGFBR1 mRNA.

Applications

Unspecified application

Species

Unspecified reactive species

Jiamin Zhou,Yu Chen,Jinfa Chen,Guojin Xia,Junyi Zeng,Liang Wang

JCI insight 10: PubMed40779453

2025

NAT10-mediated ac4C modification of Lipin1 mRNA contributes to the pathogenesis of PWMI.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyu Li,Meng Zhang,Yanan Liu,Chunjie Guo,Yiwei Liu,Lei Han,Zhaowei Feng,Xiue Wei,Ruiqin Yao

Journal of bone oncology 53:100701 PubMed40717796

2025

Yin Yang 1 protein-activated N-acetyltransferase 10 drives cell malignant progression of osteosarcoma through ac4C acetylation of integrin β3.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Yang,Mao Wang

Science advances 11:eadu0955 PubMed40680131

2025

Nicotinamide boosts oocyte quantity and quality by promoting N4-acetylation modification in lupus mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Xie,Chuanchuan Zhou,Qi Guo,Yingchun Guo,Jiayi Guo,Lina Chen,Xiaoping Liu,Yanyan Zeng,Qiqi Liang,Taibao Wu,Jiawen Liu,Xiaoyan Liang

iScience 28:112860 PubMed40606759

2025

Mechanistic study of N-acetyltransferase 10 deficiency enhancing olaparib sensitivity in triple negative breast cancer by inhibiting RAD51 N4-acetylcytidine modification.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Li,Hao Wu,Siwei Li,Qin Wang,Guozheng Li,Xin Ma,Yajie Gong,Yijun Chu,Shengye Jin,Xi Chen,Xianyu Zhang,Da Pang

Nature communications 16:5496 PubMed40595590

2025

Mammalian tRNA acetylation determines translation efficiency and tRNA quality control.

Applications

Unspecified application

Species

Unspecified reactive species

Na Liu,Bingxue Liu,Chun-Rui Ma,Zixin Cai,Jin-Tao Wang,Zi-Qing Chai,Nanlin Zhu,Ting Shao,Yue-Lei Chen,Yu Lin,Yirong Wang,Hong Xu,Xiao-Long Zhou

Cell death & disease 16:471 PubMed40593466

2025

Impact of N-acetyltransferase 10 on macrophage activation and inflammation-induced cardiac dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Zilong Xiao,Xiang Wei,Peng Li,Ruizhen Chen,Ziqing Yu,Yixiu Liang,Yangang Su,Junbo Ge

Nucleic acids research 53: PubMed40521666

2025

Nat10 maintains the homeostasis of pluripotent and 2-cell-like states in mouse embryonic stem cells through mRNA cytidine acetylation.

Applications

Unspecified application

Species

Unspecified reactive species

Guofeng Feng,Guoxing Yin,Yusheng Liu,Chang Liu,Jie Li,Jiangtao Lu,Yongqin Yu,Ziyi Jin,Yiwei Wu,Yanbin Yue,Xiufang Gao,Jiaqiang Wang,Falong Lu,Lin Liu

Nature communications 16:5154 PubMed40461504

2025

Inhibition of tumor-intrinsic NAT10 enhances antitumor immunity by triggering type I interferon response via MYC/CDK2/DNMT1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wan-Cheng Liu,Yi-Hong Wei,Jin-Feng Chen,Xiang-Ling Xing,He-Xiao Jia,Xin-Yu Yang,Ying-Jian Huang,Xiao-Min Liu,Ke Xiao,Xiao-Dong Guo,Can Can,A-Min Zhang,Na He,Hai-Lei Zhang,Dao-Xin Ma

Scientific reports 15:17286 PubMed40389420

2025

NAT10 promotes hepatocellular carcinoma progression by modulating the ac4C-DDIAS-PI3K-Akt axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Tao,Leisheng Wang,Enhong Chen,Shuo Zhang,Dongjie Yang,Wuqiang Chen,Youzhao He,Yuanlong Gu,Yong Mao,Hao Hu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128]

Dot Blot - Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] (AB252215)

Dot Blot - Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] (AB252215)

Dot Blot - Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] (AB252215)

Dot Blot - Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] (AB252215)

Dot Blot - Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] (AB252215)

Dot Blot - Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] (AB252215)

Dot Blot - Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] (AB252215)

Dot Blot - Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] (AB252215)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (68)

Product promise