AB253039

Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] - BSA and Azide free

RabMAb
Recombinant
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(5 Publications)

Rabbit Recombinant Monoclonal N4-acetylcytidine (ac4C) antibody. Carrier free. Suitable for Dot and reacts with Modified Nucleic Acid samples. Cited in 5 publications.

8 Images

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Dot Blot - Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] - BSA and Azide free (AB253039)

This data was developed using the same antibody clone in a different buffer formulation. ab252215

Competitive RNA Dot Blot - Recombinant Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] (ab252215)

Primary antibody dilution : 1 ug/ml

Secondary antibody : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated

Secondary antibody dilution : 1/20,000

Blocking buffer and dilution buffer : AdvanBlock™ Chemi Blocking buffer

Input : HeLa total RNA 500ng per Dot

Competitive nucleosides :

Lane A : ac4C

Lane B : unmodified C

Exposure time : 137 seconds

This blot was developed using a higher sensitivity ECL substrate

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Dot Blot - Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] - BSA and Azide free (AB253039)

Dot blot analysis of N4-acetylcytidine (ac4C) labeled with either ab252215 at 1/1000 dilution or with methylene blue.

Lane 1 : Total RNA purified from wild-type Hela cells.
Lane 2 : Total RNA purified from NAT10 knockout HeLa cells

Goat Anti-Rabbit IgG H&L (HRP) at 1/100000 dilution was used as secondary antibody.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times : Left panel, 2 minutes. Right panel, 30 seconds.

Knock out of NAT10 (human acetyltransferase) can dramatically reduce ac4C level.

This image was kindly provided by Dr. Jordan Meier, NCI, USA.

This antibody was cited in PMID : 29039931 and 30449621.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252215).

Lab

Dot Blot - Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] - BSA and Azide free (AB253039)

This data was developed using the same antibody clone in a different buffer formulation (ab252215).

Dot blot analysis of N4-acetylcytidine (ac4C) labeled with ab252215 at 1/200 dilution.

Lane 1 : HeLa total RNA
Lane 2 : ac4C RNA oligo
Lane 3 : C RNA oligo

Goat Anti-Rabbit IgG H&L (HRP) Peroxidase conjugated (ab97051) at 1/20,000 dilution was used as secondary antibody.

Ac4C oligo (without biotin labelled) used in the figure was provided by a collaborator.

PubMed

Dot Blot - Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] - BSA and Azide free (AB253039)

Dot blot analysis showing effect of hydroxylamine on ac4C in RNA using anti-ac4C antibody. Reaction conditions : 50 mM hydroxylamine, Tris at pH 8.0, 65 °C, 1 hour. Hydroxylamine reduced the detection of ac4C.

Refer to PMID 29039931

Image from Sinclair WR et al., ACS Chemical Biology., 12 (12), 2922-2926. Fig 4C.; doi : 10.1021/acschembio.7b00734.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252215).

Image from Sinclair WR et al., ACS Chemical Biology., 12 (12), 2922-2926. Fig 4C.; doi: 10.1021/acschembio.7b00734.

PubMed

Dot Blot - Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] - BSA and Azide free (AB253039)

Dot blot analysis of total RNA by anti-ac4C found a significantly decreased signal in hydroxylamine-treated samples compared to untreated samples.

Refer to PMID 29039931

Image from Sinclair WR et al., ACS Chemical Biology., 12 (12), 2922-2926. Fig 4F.; doi : 10.1021/acschembio.7b00734.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252215).

Image from Sinclair WR et al., ACS Chemical Biology., 12 (12), 2922-2926. Fig 4F.; doi: 10.1021/acschembio.7b00734.

Unknown

Dot Blot - Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] - BSA and Azide free (AB253039)

Dot blot analysis of N4-acetylcytidine (ac4C) labeled with ab252215 at 1/1000 dilution.

Lane 1 : Synthetic wild type Beta globin RNA probe.
Lane 2 : Synthetic Beta globin RNA probe containing ac4C.
Lane 3 : Synthetic Beta globin RNA probe containing m5C.
Lane 4 : Beta globin RNA probe containing hm5C.

Goat Anti-Rabbit IgG H&L (HRP) at 1/100000 dilution was used as secondary antibody.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 2 seconds.

This antibody shows no cross reactivity with other RNA modifications.

This image was kindly provided by Dr. Jordan Meier, NCI, USA.

This antibody was cited in PMID : 29039931 and 30449621.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252215).

PubMed

Dot Blot - Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] - BSA and Azide free (AB253039)

Dot blot analysis demonstrates concentration-dependent detection of in vitro transcribed ac4C-containing RNAs. The antibody detects RNA probes synthesized in the presence of ac4CTP, but not CTP.

Refer to PMID : 29039931.

Image from Sinclair WR et al., ACS Chemical Biology., 12 (12), 2922-2926. Fig 3C.; doi : 10.1021/acschembio.7b00734.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252215).

Image from Sinclair WR et al., ACS Chemical Biology., 12 (12), 2922-2926. Fig 3C.; doi: 10.1021/acschembio.7b00734.

Unknown

Dot Blot - Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] - BSA and Azide free (AB253039)

Dot blot analysis of N4-acetylcytidine (ac4C) labeled with ab252215 at 1/1000 dilution.

Lane 1 : Synthetic wild type E. coli tRNA Met probe.
Lane 2 : Synthetic E. coli tRNA Met probe containing ac4C.
Lane 3 : Synthetic E. coli tRNA Met probe containing ac4C pre-treated with 50mM hydroxylamine.

Goat Anti-Rabbit IgG H&L (HRP) at 1/100000 dilution was used as secondary antibody.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 2 seconds.

Hydroxylamine removes the electrophilic acetamide of ac4C.

This image was kindly provided by Dr. Jordan Meier, NCI, USA.

This antibody was cited in PMID : 29039931 and 30449621.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252215).

Unconjugated

Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPRNCI-184-128

Isotype

IgG

Carrier free

Yes

Applications

Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Modified Nucleic Acid": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>" } } }

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Product details

ab253039 is the carrier-free version of ab252215.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

N4-acetylcytidine (ac4C) a modified nucleoside occurs in various types of RNA like tRNA rRNA and mRNA. The molecular mass of ac4C roughly totals 255 daltons. It is expressed ubiquitously across many organisms including humans yeast and bacteria. This modification also referred to sometimes as an 'RNA acetylation mark' contributes to the stability and codon-anticodon interactions in tRNA with an important role in maintaining the structural integrity of rRNA components.

Biological function summary

N4-acetylcytidine acts to enhance translation fidelity which is essential for accurate gene expression. It ensures the stabilization of RNA structures and impacts the efficiency of translation. This modification often involves an enzyme complex including acetyltransferases which work by transferring the acetyl group from acetyl-CoA to the cytidine residue. Proper functioning and balance of ac4C modifications in RNA depend on these enzymes ensuring that the RNA molecule remains functional and effective in protein synthesis.

Pathways

N4-acetylcytidine takes part in translation and RNA processing pathways. It works alongside RNA polymerase complexes and other enzymes that modify RNA. The modification of ac4C enhances the initiation and elongation phases of translation. Within these pathways it interacts with proteins such as RNA acetylating enzymes and ribosomal proteins to fine-tune these processes ensuring that RNA synthesis and subsequent protein production are precise and efficient.

N4-acetylcytidine shows links to conditions related to translation errors and cellular stress responses. Aberrant levels of ac4C have connections to cancer wherein dysregulated translation and misfolded protein formation occur. Defective ac4C modification might lead to neurodegeneration where accurate protein synthesis is compromised. Proteins like NAT10 a known RNA acetyltransferase interact with ac4C in these pathological contexts indicating the importance of maintaining balanced ac4C levels for cellular health and disease prevention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information N4-acetylcytidine (ac4C)

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

CytoJournal 21:68 PubMed39917001

2025

N-acetyltransferase 10 regulates UNC-51-like kinase 1 to reduce tubular cell injury and kidney stone formation.

Applications

Unspecified application

Species

Unspecified reactive species

Le Wang,Jinjing Huang,Lei Song,Ben Ke

Journal of cellular and molecular medicine 28:e70141 PubMed39482983

2024

NAT10-mediated RNA ac4C acetylation contributes to the myocardial infarction-induced cardiac fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Li,Feierkaiti Yushanjiang,Zhao Fang,Wan-Li Liu

Redox biology 72:103145 PubMed38583415

2024

The positive feedback loop of the NAT10/Mybbp1a/p53 axis promotes cardiomyocyte ferroptosis to exacerbate cardiac I/R injury.

Applications

Unspecified application

Species

Unspecified reactive species

Zhezhe Qu,Xiaochen Pang,Zhongting Mei,Ying Li,Yaozhi Zhang,Chuanhao Huang,Kuiwu Liu,Shuting Yu,Changhao Wang,Zhiyong Sun,Yingqi Liu,Xin Li,Yingqiong Jia,Yuechao Dong,Meixi Lu,Tiantian Ju,Fan Wu,Min Huang,Na Li,Shunkang Dou,Jianhao Jiang,Xianhui Dong,Yi Zhang,Wanhong Li,Baofeng Yang,Weijie Du

Cell communication and signaling : CCS 22:51 PubMed38233839

2024

NAT10 mediated ac4C acetylation driven mA modification via involvement of YTHDC1-LDHA/PFKM regulates glycolysis and promotes osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongting Mei,Zhihua Shen,Jiaying Pu,Qian Liu,Guoxin Liu,Xuting He,Yang Wang,Jinrui Yue,Shiyu Ge,Tao Li,Ye Yuan,Lei Yang

PLoS pathogens 17:e1009940 PubMed34543359

2021

PCV2 targets cGAS to inhibit type I interferon induction to promote other DNA virus infection.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenyu Wang,Jing Chen,Xingchen Wu,Dan Ma,Xiaohua Zhang,Ruizhen Li,Cong Han,Haixin Liu,Xiangrui Yin,Qian Du,Dewen Tong,Yong Huang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] - BSA and Azide free

Dot Blot - Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] - BSA and Azide free (AB253039)

Dot Blot - Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] - BSA and Azide free (AB253039)

Dot Blot - Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] - BSA and Azide free (AB253039)

Dot Blot - Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] - BSA and Azide free (AB253039)

Dot Blot - Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] - BSA and Azide free (AB253039)

Dot Blot - Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] - BSA and Azide free (AB253039)

Dot Blot - Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] - BSA and Azide free (AB253039)

Dot Blot - Anti-N4-acetylcytidine (ac4C) antibody [EPRNCI-184-128] - BSA and Azide free (AB253039)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise