AB211494

Anti-N4-methylcytidine (m4C) antibody [EPR -19850-108]

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal N4-methylcytidine (m4C) antibody. Suitable for IP, ELISA and reacts with Modified Nucleic Acid samples. Cited in 1 publication.

View Alternative Names

N4-methylcytidine, m4C

2 Images

ELISA

Supplier Data

ELISA - Anti-N4-methylcytidine (m4C) antibody [EPR -19850-108] (AB211494)

Biotinylated m4C (modified) and C (unmodified) oligonucleotides with the below sequence were coated onto wells of a 96 well plate.

Modified oligonucleotide (5 μM) 5' Biotin-mN.mN.mN.mN.mN.[m4C]*.mN.mN.mN.mN.mN 3'

Unmodified oligonucleotide (5 μM) 5' Biotin-mN.mN.mN.mN.mN.[C]*.mN.mN.mN.mN.mN 3'

N - equimolar mixture of (A/U/G/C)
m - 2'O methyl protection
* - phosphorothioate protection

ELISA was performed on 1.0 μg/ml of antigen using ab211494 at a concentration range of 0.005-4.000 μg/ml followed by Goat Anti-Rabbit IgG (H+L) alkaline phosphatase conjugated secondary antibody at 1/2500 dilution.

Supplier Data

Immunoprecipitation - Anti-N4-methylcytidine (m4C) antibody [EPR -19850-108] (AB211494)

The IP was performed in a U-bottom non-adsorbing propylene 96-well plate.

ab211494 (0.2 μg) was coated into Dynabeads® sheep-anti-rabbit IgG (50 μl) for 1h at RT.

Unmodified/modified oligonucleotides (5 μM) were added to samples containing the antibody/bead complexes and incubated with agitation for 1 hour at RT.

After washing, Peroxidase-conjugated Streptavidin was incubated at 1/1000 dilution with agitation for 1 hour at RT.

ECL substrate was then added and the results read in a non-transparent 96-well plate with a digital detector and analyzed using ImageJ.

Lane 1 : Buffer only.

Lane 2 : Modified oligonucleotide (5 μM), 5' Biotin-mN.mN.mN.mN.mN.[m4C]*.mN.mN.mN.mN.mN 3'

Lane 3 : Unmodified oligonucleotide (5 μM), 5' Biotin-mN.mN.mN.mN.mN.[C]*.mN.mN.mN.mN.mN 3'

N - equimolar mixture of (A/U/G/C)
m - 2'O methyl protection
* - phosphorothioate protection

Blocking buffer and concentration : 5% NFDM/TBST

Dilution buffer and concentration : TBST/0.1% Triton X-100/1 mM EDTA

All lanes:

Immunoprecipitation - Anti-N4-methylcytidine (m4C) antibody [EPR -19850-108] (ab211494)

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Carrier free

Anti-N4-methylcytidine (m4C) antibody [EPR -19850-108] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR -19850-108

Isotype

IgG

Carrier free

Applications

ELISA, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Has been developed to discriminate between the modified base N4-methylcytidine (m4C) and the unmodified counterpart Cytidine (C).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Modified Nucleic Acid": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "0.2 μg of ab211494 was coated onto 50 μL Dynabeads® sheep-anti-rabbit IgG.", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "0.005-4 µg/mL", "ELISA-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

N4-methylcytidine (m4C) is a modified nucleoside found in RNA specifically involved in post-transcriptional modifications. It is sometimes referred to as methylcytidine and its mass is approximately 257.23 Da. You can find m4C expressed in various prokaryotic and eukaryotic systems suggesting its broad role in molecular biology. Notably the methylation process leading to m4C is facilitated by specific methyltransferase enzymes. Because of this enzymatic activity the nucleoside exhibits diverse mechanical functions that influence RNA stability and translation efficiency.

Biological function summary

M4C plays a role in the regulation of gene expression. It is normally observed as part of the ribosomal RNA in both bacterial and mammalian cells indicating its conserved function across species. The methylation at the cytidine position in RNA helps stabilize the RNA structure which contributes to the control of protein synthesis. m4C does not directly form part of any known complex but the modifications it undergoes indirectly affect ribosomal complexes and their function.

Pathways

The modifications introduced by m4C are fundamental to the mRNA translation pathway and RNA processing pathway. The methylated form interacts with ribosomal proteins influencing translational fidelity and efficiency. m4C also associates with RNA polymerase proteins due to its participation in message stability. These interactions position m4C as a pivotal contributor in maintaining cellular homeostasis by regulating protein synthesis through these essential pathways.

M4C modifications relate to certain types of cancers and neurological disorders. Abnormal m4C levels can disrupt normal gene expression patterns contributing to oncogenesis. Furthermore its association with ribosomal processing implicates it in neurodegenerative diseases where protein misfolding is a characteristic feature. Proteins like ribosomal protein S5 and transcription factor E2F1 have connections to m4C's altered functions in these conditions revealing the complex role m4C plays in disease etiology. Understanding m4C's involvement might help in developing targeted therapies for conditions linked to translation deregulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information N4-methylcytidine (m4C)

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular neurodegeneration 20:103 PubMed41035073

2025

Molecular hallmarks of excitatory and inhibitory neuronal resilience to Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Isabel Castanho,Pourya Naderi Yeganeh,Carles A Boix,Sarah L Morgan,Hansruedi Mathys,Dmitry Prokopenko,Bartholomew White,Larisa M Soto,Giulia Pegoraro,Saloni Shah,Athanasios Ploumakis,Nikolas Kalavros,David A Bennett,Christoph Lange,Doo Yeon Kim,Lars Bertram,Li-Huei Tsai,Manolis Kellis,Rudolph E Tanzi,Winston Hide

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-N4-methylcytidine (m4C) antibody [EPR -19850-108]

ELISA - Anti-N4-methylcytidine (m4C) antibody [EPR -19850-108] (AB211494)

Immunoprecipitation - Anti-N4-methylcytidine (m4C) antibody [EPR -19850-108] (AB211494)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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