Anti-N4-methylcytidine (m4C) antibody [EPR -19850-108]

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

N4-methylcytidine (m4C) is a modified nucleoside found in RNA specifically involved in post-transcriptional modifications. It is sometimes referred to as methylcytidine and its mass is approximately 257.23 Da. You can find m4C expressed in various prokaryotic and eukaryotic systems suggesting its broad role in molecular biology. Notably the methylation process leading to m4C is facilitated by specific methyltransferase enzymes. Because of this enzymatic activity the nucleoside exhibits diverse mechanical functions that influence RNA stability and translation efficiency.

Biological function summary

M4C plays a role in the regulation of gene expression. It is normally observed as part of the ribosomal RNA in both bacterial and mammalian cells indicating its conserved function across species. The methylation at the cytidine position in RNA helps stabilize the RNA structure which contributes to the control of protein synthesis. m4C does not directly form part of any known complex but the modifications it undergoes indirectly affect ribosomal complexes and their function.

Pathways

The modifications introduced by m4C are fundamental to the mRNA translation pathway and RNA processing pathway. The methylated form interacts with ribosomal proteins influencing translational fidelity and efficiency. m4C also associates with RNA polymerase proteins due to its participation in message stability. These interactions position m4C as a pivotal contributor in maintaining cellular homeostasis by regulating protein synthesis through these essential pathways.

Associated diseases and disorders

M4C modifications relate to certain types of cancers and neurological disorders. Abnormal m4C levels can disrupt normal gene expression patterns contributing to oncogenesis. Furthermore its association with ribosomal processing implicates it in neurodegenerative diseases where protein misfolding is a characteristic feature. Proteins like ribosomal protein S5 and transcription factor E2F1 have connections to m4C's altered functions in these conditions revealing the complex role m4C plays in disease etiology. Understanding m4C's involvement might help in developing targeted therapies for conditions linked to translation deregulation.