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AB122522

Anti-N42L1 antibody

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Rabbit Polyclonal N42L1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human N4BP2L1 aa 150 to C-terminus.

View Alternative Names

CG081, N4BP2L1, NEDD4-binding protein 2-like 1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N42L1 antibody (AB122522)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N42L1 antibody (AB122522)

Paraffin-embedded formalin-fixed human cerebellum tissue stained for N4BP2L1 using ab122522 at 1/200 dilution in immunohistochemical analysis.

Western blot - Anti-N42L1 antibody (AB122522)
  • WB

Unknown

Western blot - Anti-N42L1 antibody (AB122522)

All lanes:

Western blot - Anti-N42L1 antibody (ab122522) at 0.4 µg/mL

Lane 1:

Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Lane 2:

N4BP2L1 over-expression HEK-293T whole cell lysate

Predicted band size: 29 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human N4BP2L1 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q5TBK1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

N42L1 also known as Olfactomedin-like 2B (OLFML2B) is a protein found across various tissues in the body with significant expression in the brain and reproductive tissues. It is a member of the olfactomedin family and has a molecular mass of approximately 62 kDa. N42L1 acts as a secreted glycoprotein and possesses olfactomedin domains which are believed to contribute to extracellular matrix organization. The protein is widely studied for its biochemical properties including its role in cellular adhesion processes.
Biological function summary

N42L1 contributes to cellular adhesion and tissue development through interaction with other cellular elements. It plays a part in the formation and stabilization of extracellular matrix likely through its oligomeric state indicating it may function as part of a protein complex. N42L1 influences cell signaling by interacting with growth factors and adhesion molecules impacting cellular communication and maintenance of tissue integrity.

Pathways

N42L1 becomes important within the extracellular matrix organization and cell adhesion pathways. It associates with proteins like fibronectin which further links to integrin signaling pathways. These interactions place N42L1 within important networks that regulate tissue architecture and cellular communication. The involvement of N42L1 in lamellar bodies suggests a role in lipid processing pathways which connect to several other structural proteins modulating matrix conditions.

Research links N42L1 to disorders such as glaucoma and certain cancers. Its expression and interaction with other proteins like myocilin appear altered in these conditions. In glaucoma disruption in the homeostasis and integrity of the extracellular matrix involving N42L1 and myocilin appears as a contributing factor. Regarding cancer studies suggest that changes in N42L1 expression can influence tumor progression due to its role in cell adhesion thereby making it a protein of interest in oncological research.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information N4BP2L1
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