AB106649

Anti-N4BP1 antibody

Name: Anti-N4BP1 antibody (ab106649) | Abcam
Brand: Abcam Limited
SKU: AB106649

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Polyclonal N4BP1 antibody. Suitable for ICC, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human N4BP1.

View Alternative Names

KIAA0615, N4BP1, NEDD4-binding protein 1

3 Images

Unknown

Immunocytochemistry - Anti-N4BP1 antibody (AB106649)

Immunofluorescence of N4BP1 in HeLa cells with ab106649 at 20 ug/mL.

Unknown

Immunocytochemistry - Anti-N4BP1 antibody (AB106649)

Immunocytochemistry of N4BP1 in HeLa cells with ab106649 at 10 ug/mL.

Supplier Data

Western blot - Anti-N4BP1 antibody (AB106649)

Primary incubation for 1 hour at room temperature in 5% NFDM/TBST.

All lanes:

Western blot - Anti-N4BP1 antibody (ab106649) at 1 µg/mL

Lane 1:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 15 µg

Lane 2:

K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) at 1/10000 dilution

Predicted band size: 100 kDa

false

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC, WB

applications

Immunogen

Synthetic Peptide within Human N4BP1. The exact immunogen used to generate this antibody is proprietary information.

O75113

Specificity

At least three isoforms of N4BP1 are known to exist; ab106649 will only detect the longest isoform.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "10-20 µg/mL", "ICC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

N4BP1 also known as NEDD4-binding protein 1 is a protein with a molecular mass of approximately 67 kDa. It is ubiquitously expressed with notable levels in kidney liver and brain tissues. Mechanically N4BP1 acts as an adaptor molecule involved in protein-protein interactions especially noted for its role in ubiquitination processes. The protein has interactions with the E3 ubiquitin ligase NEDD4 influencing protein turnover and cellular homeostasis.

Biological function summary

N4BP1 plays a role in cellular signaling pathways that maintain protein stability and degradation. It is part of multiprotein complexes mediating ubiquitin-dependent protein processing. Through these complexes N4BP1 participates in the regulation of protein levels impacting various cellular processes such as cell cycle progression and stress response.

Pathways

N4BP1 is integral to the ubiquitin-proteasome system a critical pathway for protein catabolism in cells. It interacts with components of the NEDD4-mediated pathway impacting proteins such as SMAD4. This modulation affects signal transduction processes that control cellular fate decisions. Additionally N4BP1 is involved in pathways regulating inflammatory responses linking it to immune response modulation.

N4BP1 has associations with cancer and neurodegenerative diseases. Alterations in N4BP1's function or expression can affect the ubiquitination process influencing tumor suppressor proteins and cellular proliferation in cancer. In neurodegenerative conditions N4BP1's interactions with ubiquitin-related proteins such as NEDD4 play a role in protein aggregation dynamics contributing to disease pathology by potentially impacting the clearance of misfolded proteins.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Potent suppressor of cytokine production that acts as a regulator of innate immune signaling and inflammation. Acts as a key negative regulator of select cytokine and chemokine responses elicited by TRIF-independent Toll-like receptors (TLRs), thereby limiting inflammatory cytokine responses to minor insults. In response to more threatening pathogens, cleaved by CASP8 downstream of TLR3 or TLR4, leading to its inactivation, thereby allowing production of inflammatory cytokines (By similarity). Acts as a restriction factor against some viruses, such as HIV-1 : restricts HIV-1 replication by binding to HIV-1 mRNAs and mediating their degradation via its ribonuclease activity (PubMed : 31133753). Also acts as an inhibitor of the E3 ubiquitin-protein ligase ITCH : acts by interacting with the second WW domain of ITCH, leading to compete with ITCH's substrates and impairing ubiquitination of substrates (By similarity).

See full target information N4BP1

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

World journal of surgical oncology 21:62 PubMed36841776

2023

Low expression of ZSCAN4 predicts unfavorable outcome in urothelial carcinoma of upper urinary tract and urinary bladder.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Lin He,Hong-Yue Lai,Ti-Chun Chan,Chung-Hsi Hsing,Steven K Huang,Kun-Lin Hsieh,Tzu-Ju Chen,Wan-Shan Li,Yu-Hsuan Kuo,Yow-Ling Shiue,Chien-Feng Li

The Journal of experimental medicine 218: PubMed34406363

2021

Cyclin D2 overexpression drives B1a-derived MCL-like lymphoma in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Tim Pieters,Sara T'Sas,Stijn Vanhee,André Almeida,Yasmine Driege,Juliette Roels,Wouter Van Loocke,Willem Daneels,Mathijs Baens,Arnaud Marchand,Maaike Van Trimpont,Filip Matthijssens,Julie Morscio,Kelly Lemeire,Béatrice Lintermans,Lindy Reunes,Patrick Chaltin,Fritz Offner,Jo Van Dorpe,Tino Hochepied,Geert Berx,Rudi Beyaert,Jens Staal,Pieter Van Vlierberghe,Steven Goossens

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-N4BP1 antibody

Immunocytochemistry - Anti-N4BP1 antibody (AB106649)

Immunocytochemistry - Anti-N4BP1 antibody (AB106649)

Western blot - Anti-N4BP1 antibody (AB106649)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise