AB133610

Anti-N4BP1 antibody [EPNCIR118]

RabMAb
Recombinant
What is this?

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(5 Publications)

Rabbit Recombinant Monoclonal N4BP1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Rat samples. Cited in 5 publications.

View Alternative Names

Kiaa0615, NEDD4-binding protein 1, N4BP1

2 Images

Flow Cytometry (Intracellular) - Anti-N4BP1 antibody [EPNCIR118] (AB133610)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-N4BP1 antibody [EPNCIR118] (AB133610)

Intracellular flow cytometrical analysis of N4BP1 in permeabilized F9 cells, using ab133610 at a dilution of 1/100 (red) or a rabbit IgG (negative) (green).

Unknown

Western blot - Anti-N4BP1 antibody [EPNCIR118] (AB133610)

All lanes:

Western blot - Anti-N4BP1 antibody [EPNCIR118] (ab133610) at 1/1000 dilution

Lane 1:

F9 cell lysate at 10 µg

Lane 2:

Mouse brain cell lysate at 10 µg

Lane 3:

B16 F0 cell lysate at 10 µg

Lane 4:

PC12 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 100 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPNCIR118

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Michael Kuehn. View antibodies from NCI Center for Cancer Research Collaboration.

Species reactivity
Human: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

N4BP1 also known as NEDD4-binding protein 1 is a protein with a molecular mass of approximately 67 kDa. It is ubiquitously expressed with notable levels in kidney liver and brain tissues. Mechanically N4BP1 acts as an adaptor molecule involved in protein-protein interactions especially noted for its role in ubiquitination processes. The protein has interactions with the E3 ubiquitin ligase NEDD4 influencing protein turnover and cellular homeostasis.

Biological function summary

N4BP1 plays a role in cellular signaling pathways that maintain protein stability and degradation. It is part of multiprotein complexes mediating ubiquitin-dependent protein processing. Through these complexes N4BP1 participates in the regulation of protein levels impacting various cellular processes such as cell cycle progression and stress response.

Pathways

N4BP1 is integral to the ubiquitin-proteasome system a critical pathway for protein catabolism in cells. It interacts with components of the NEDD4-mediated pathway impacting proteins such as SMAD4. This modulation affects signal transduction processes that control cellular fate decisions. Additionally N4BP1 is involved in pathways regulating inflammatory responses linking it to immune response modulation.

N4BP1 has associations with cancer and neurodegenerative diseases. Alterations in N4BP1's function or expression can affect the ubiquitination process influencing tumor suppressor proteins and cellular proliferation in cancer. In neurodegenerative conditions N4BP1's interactions with ubiquitin-related proteins such as NEDD4 play a role in protein aggregation dynamics contributing to disease pathology by potentially impacting the clearance of misfolded proteins.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Potent suppressor of cytokine production that acts as a regulator of innate immune signaling and inflammation (PubMed : 32971525). Acts as a key negative regulator of select cytokine and chemokine responses elicited by TRIF-independent Toll-like receptors (TLRs), thereby limiting inflammatory cytokine responses to minor insults (PubMed : 32971525). In response to more threatening pathogens, cleaved by CASP8 downstream of TLR3 or TLR4, leading to its inactivation, thereby allowing production of inflammatory cytokines (PubMed : 32971525). Acts as a restriction factor against some viruses : restricts viral replication by binding to mRNA viruses and mediating their degradation via its ribonuclease activity (By similarity). Also acts as an inhibitor of the E3 ubiquitin-protein ligase ITCH : acts by interacting with the second WW domain of ITCH, leading to compete with ITCH's substrates and impairing ubiquitination of substrates (PubMed : 17592138).

See full target information N4bp1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Immunity 57:973-986.e7 PubMed38697117

2024

N4BP1 coordinates ubiquitin-dependent crosstalk within the IκB kinase family to limit Toll-like receptor signaling and inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander D Gitlin,Allie Maltzman,Yuzuka Kanno,Klaus Heger,Rohit Reja,Alexander F Schubert,Linsey J Wierciszewski,Homer Pantua,Sharookh B Kapadia,Seth F Harris,Joshua D Webster,Kim Newton,Vishva M Dixit

Experimental and therapeutic medicine 26:577 PubMed38023354

2023

Alendronate augments lipid A‑induced IL‑1β release by ASC‑deficient RAW264 cells via AP‑1 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Noriyuki Watanabe,Riyoko Tamai,Yusuke Kiyoura

The EMBO journal 42:e113383 PubMed37807845

2023

N4BP1 mediates RAM domain-dependent notch signaling turnover during neocortical development.

Applications

Unspecified application

Species

Unspecified reactive species

Zhihua Ma,Yi Zeng,Ming Wang,Wei Liu,Jiafeng Zhou,Chao Wu,Lin Hou,Bin Yin,Boqin Qiang,Pengcheng Shu,Xiaozhong Peng

Gut microbes 15:2163838 PubMed36656595

2023

An IBD-associated pathobiont synergises with NSAID to promote colitis which is blocked by NLRP3 inflammasome and Caspase-8 inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Raminder Singh,Valerio Rossini,Stephen R Stockdale,Gonzalo Saiz-Gonzalo,Naomi Hanrahan,Tanya D' Souza,Adam Clooney,Lorraine A Draper,Colin Hill,Ken Nally,Fergus Shanahan,Stefan Andersson-Engels,Silvia Melgar

Scientific reports 9:18486 PubMed31811168

2019

RAG-2 deficiency results in fewer phosphorylated histone H2AX foci, but increased retinal ganglion cell death and altered axonal growth.

Applications

Unspecified application

Species

Unspecified reactive species

Noemí Álvarez-Lindo,Jimena Baleriola,Vivian de Los Ríos,Teresa Suárez,Enrique J de la Rosa

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-N4BP1 antibody [EPNCIR118]

Flow Cytometry (Intracellular) - Anti-N4BP1 antibody [EPNCIR118] (AB133610)

Western blot - Anti-N4BP1 antibody [EPNCIR118] (AB133610)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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