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AB208577

Anti-N6-methyladenosine (m6A) antibody [17-3-4-1]

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(174 Publications)

Anti-N6-methyladenosine (m6A) antibody [17-3-4-1] (ab208577) is a mouse monoclonal antibody detecting N6-methyladenosine (m6A) in IP.

- Over 60 publications

View Alternative Names

N6-methyladenosine, m6A

3 Images
Immunoprecipitation - Anti-N6-methyladenosine (m6A) antibody [17-3-4-1] (AB208577)
  • IP

Supplier Data

Immunoprecipitation - Anti-N6-methyladenosine (m6A) antibody [17-3-4-1] (AB208577)

N6-methyladenosine (m6A) was immunoprecipitated using ab208577 from radiolabeled synthetic methylated transcript (Bound); radiolabeled Non methylated control (Non bound) and radiolabeled Input RNA control.

All lanes:

Immunoprecipitation - Anti-N6-methyladenosine (m6A) antibody [17-3-4-1] (ab208577)

false

Southern Blot - Anti-N6-methyladenosine (m6A) antibody [17-3-4-1] (AB208577)
  • SB

Supplier Data

Southern Blot - Anti-N6-methyladenosine (m6A) antibody [17-3-4-1] (AB208577)

Lane 1 Single stranded DNA

Lane 2 Double stranded DNA

Labelled with ab208577. Arrow shows m6A containing fragment.

Northwestern blot - Anti-N6-methyladenosine (m6A) antibody [17-3-4-1] (AB208577)
  • Northwestern

Supplier Data

Northwestern blot - Anti-N6-methyladenosine (m6A) antibody [17-3-4-1] (AB208577)

Use of ab208577 for northwestern blot using synthetic methlyated and non methlyated RNA.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

17-3-4-1

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Applications

SB, IP, Northwestern

applications

Specificity

This antibody recognises N6-methyladenosine in both modified RNA and DNA.

Reactivity data

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Product details

What is this antibody validated in?
Anti-N6-methyladenosine (m6A) antibody [17-3-4-1] (ab208577) is a mouse monoclonal antibody and is validated for use in Immunoprecipitation (IP). Trusted by the scientific community
Anti-N6-methyladenosine (m6A) [17-3-4-1] (ab208577) was first used in a scientific publication in 2015 and has been cited over 60 times in peer-reviewed journals.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

N6-methyladenosine (m6A) is a chemical modification present in RNA molecules specifically marking the adenosine base with a methyl group at the nitrogen-6 position. This modification weighing approximately 14 Da occurs in diverse eukaryotic species and influences RNA metabolism. m6A methylation can be detected using techniques like m6A dots blot or m6A ELISA. The modification is abundant in tissues like the brain and testis reflecting its critical role in varying cell types. Also m6A is known as a dynamic and reversible mark with its levels continuously adjusted by enzymes called writers (methyltransferases) erasers (demethylases) and readers (RNA-binding proteins).
Biological function summary

M6A methylation affects mRNA processing stability translation and decay. It integrates into large multi-protein complexes where it influences gene expression outcomes by affecting the RNA's interaction with the cellular machinery. This methylation modification acts as a regulatory signal that influences essential processes such as cell differentiation and circadian rhythms. Elucidating the biological functions of m6A involves studying how it affects RNA fate and its downstream gene regulatory networks.

Pathways

M6A modification is central to the mRNA metabolic pathway and the PI3K-Akt signaling pathway. It interacts with various proteins such as METTL3 an m6A methyltransferase which is vital for mediating m6A modification. It also interacts with YTH domain-containing proteins that recognize m6A marks influencing transcript dynamics and gene expression. The interplay of m6A with proteins in these pathways underlines its role in fine-tuning cellular processes and responses.

M6A modification has a significant impact on cancer and neurological disorders. In cancers alterations in m6A methylation patterns can promote oncogenic transformation and metastasis. m6A-related proteins such as FTO an m6A demethylase have shown connections to these pathways affecting cancer progression. In neurological disorders m6A impacts aspects of neural development and function and abnormalities in its regulation may contribute to diseases like Alzheimer's. Understanding the roles of m6A in diverse diseases can pave the way for novel therapeutic approaches.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Publications (174)

Recent publications for all applications. Explore the full list and refine your search

Kidney diseases (Basel, Switzerland) 11:543-559 PubMed41064102

2025

Dihydroquercetin/Taxifolin Inhibits METTL3/mA/PIM2 Expression by Suppressing RIPK2 Phosphorylation Mediated NF-κB p65 Signaling Pathway to Alleviates Renal Ischemia-Reperfusion Injury in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Sheng Shen,Xiao Wang,Zhongda Li,Jinyang Zhuang,Keqin Zhang

Journal of cellular and molecular medicine 29:e70829 PubMed40913254

2025

METTL3 Silencing Suppresses Cardiac Fibrosis Post Myocardial Infarction via m6A Modification of SMOC2.

Applications

Unspecified application

Species

Unspecified reactive species

Yanru He,Xiaodong Pan,Zhuyuan Liu,Pengfei Zuo,Zulong Sheng,Chunshu Hao,Zaixiao Tao,Zhongpu Chen,Jiali Song,Genshan Ma,Sunkai Ling

Cell death discovery 11:417 PubMed40883266

2025

METTL3-mediated m6A modification of circCDKAL1 regulates macrophage M1 polarization and nasal epithelial cell barrier function in allergic rhinitis through IGF2BP2/JARID2/HMGB1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jiabin Zhan,Dan Luo,Yunlong Fu,Yu Zhou,Rui Li,Xin Wei

Cell & bioscience 15:124 PubMed40877964

2025

Enriched environment inhibits GLT-1 ubiquitination by downregulating SMURF1 to attenuate ischemic brain injury induced excitotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Zhang,Wen Liu,Tao Huang,Lingling Liu,Xiuping Chen

BMC biology 23:257 PubMed40817234

2025

m6A-modified circZNF548 regulates exosomal miR-7108-3p to activate CD3CD8 T cells and suppress NSCLC growth by JMY.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Long Zhao,You-Jie Li,Chun-Xia Wu,Ning Xie,Xi-Yan Li,Dian-Chao Cao,Yang Guo,Yan Liang,Yan-Mei Li,Jiang-Nan Xue,Hong-Fang Sun,Qin Wang,Xiao-Hua Li,Ping-Yu Wang,Shu-Yang Xie

The world journal of men's health : PubMed40676888

2025

Comprehensive Analysis of N6-Methyladenosine Modification Profiling in Diabetic Erectile Dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Penghui Yuan,Wenjia Deng,Honggang Cao,Yipiao Liu,Lingang Cui,Teng Li,Qingjun Meng,Taotao Sun

Hereditas 162:123 PubMed40640957

2025

METTL3 mediates m6A methylation modification of ULBP2 and affects the progression of cervical cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hongtao Ren,Yuting Wang,Jiao Yu,Lei An,Xiulong Ma,Jiyuan Pan

Scientific reports 15:24858 PubMed40640307

2025

KIAA1429 promotes non-small-cell lung cancer cell proliferation through the TRERNA1/HOXA6 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yunxia Li,Shiyao Tian,Biao Yang

Cell biology international 49:1290-1300 PubMed40600388

2025

YTHDF1-Mediated mA Methylation of GLUT1 Promotes Progress and Suppresses Propranolol Sensitivity in Infantile Hemangioma.

Applications

Unspecified application

Species

Unspecified reactive species

Yunfeng Du,Pengyuan Li,Xianghong Du

Molecular biomedicine 6:44 PubMed40555877

2025

Leucine-rich pentatricopeptide repeat-containing protein (LRPPRC)-stabilized lncRNA small nucleolar RNA host gene 15 (Snhg15) modulates hematopoietic injury induced by γ-ray irradiation via mA modification.

Applications

Unspecified application

Species

Unspecified reactive species

Shuqin Zhang,Yajia Cheng,Yujia Gao,Feifei Xu,Yuna Wang,Junling Zhang,Yue Shang,Deguan Li,Saijun Fan
View all publications

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