AB151230

Anti-N6-methyladenosine (m6A) antibody

Name: Anti-N6-methyladenosine (m6A) antibody - carrier free (ab151230) | Abcam
Brand: Abcam Limited
SKU: AB151230
Price: 400 USD
Availability: InStock
Rating: 5 (3 reviews)

(3 Reviews)

(217 Publications)

Anti-N6-methyladenosine (m6A) antibody (ab151230) is a rabbit polyclonal antibody detecting N6-methyladenosine (m6A).

- Over 110 publications

View Alternative Names

N6-methyladenosine, m6A

2 Images

Nucleotide Array

Lab

Nucleotide Array - Anti-N6-methyladenosine (m6A) antibody (AB151230)

All batches of ab151230 are tested in Nucleotide Array against N6-methyladenosine (m6A) and unmodified adenosine. Six dilutions of each oligomer are printed on to the Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to N6-methyladenosine, indicating that this antibody specifically recognises adenosine methylated at position N6.

2Ome-m6A - N6-methyladenosine

2Ome-m6A_unmod - unmodified adenosine

Lab

Western blot - Anti-N6-methyladenosine (m6A) antibody (AB151230)

Western blot performed under reducing conditions.

All lanes:

Western blot - Anti-N6-methyladenosine (m6A) antibody (ab151230) at 1 µg/mL

Lane 1:

N6-Methyladenosine conjugated BSA at 0.1 µg

Lane 2:

Adenosine conjugated BSA at 0.1 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

Predicted band size: 75 kDa

Observed band size: 75 kDa

true

Exposure time: 1min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Applications

Nucleotide Array, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

N6-methyladenosine (m6A) is a post-transcriptional modification of RNA. m6A modification has been identified in all classes of RNA (rRNA, tRNA and mRNA) and is catalysed by an evolutionary conserved multi-subunit enzyme, methyltransferase like 3 (METTL3). Cellular and viral RNA has been known to be methylated for decades. Recent studies have found that mRNA is predominately m6A modified at stop codons and long internal exons, which are conserved between mouse and human. Emerging evidence suggests m6A plays an important role in regulating gene expression, alternative splicing patterns, downstream signalling (p53) as well as apoptosis.

The regulation of m6A modifications in mRNA has been linked to disease, where fat mass and obesity-associated (FTO) has been has been reported to be a obesity risk gene. FTO is a m6A demethylase and polymorphisms that result in increased FTO expression are associated with increased body mass and risk of obesity.

Product Specifications
Anti-N6-methyladenosine (m6A) antibody (ab151230) is a rabbit polyclonal antibody and is validated for use in Nucleotide Array.
Anti-N6-methyladenosine (m6A) antibody (ab151230) specifically detects N6-methyladenosine (m6A) (UniProt ID: ; Molecular weight: kDa) and is sold in 100 µg selling sizes.

Quality and Validation
Abcam's high quality validation processes ensure Anti-N6-methyladenosine (m6A) antibody (ab151230) has high sensitivity and specificity.
Anti-N6-methyladenosine (m6A) antibody (ab151230) has been cited over 111 times in peer reviewed journals and is trusted by the scientific community.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

N6-methyladenosine (m6A) is a chemical modification present in RNA molecules specifically marking the adenosine base with a methyl group at the nitrogen-6 position. This modification weighing approximately 14 Da occurs in diverse eukaryotic species and influences RNA metabolism. m6A methylation can be detected using techniques like m6A dots blot or m6A ELISA. The modification is abundant in tissues like the brain and testis reflecting its critical role in varying cell types. Also m6A is known as a dynamic and reversible mark with its levels continuously adjusted by enzymes called writers (methyltransferases) erasers (demethylases) and readers (RNA-binding proteins).

Biological function summary

M6A methylation affects mRNA processing stability translation and decay. It integrates into large multi-protein complexes where it influences gene expression outcomes by affecting the RNA's interaction with the cellular machinery. This methylation modification acts as a regulatory signal that influences essential processes such as cell differentiation and circadian rhythms. Elucidating the biological functions of m6A involves studying how it affects RNA fate and its downstream gene regulatory networks.

Pathways

M6A modification is central to the mRNA metabolic pathway and the PI3K-Akt signaling pathway. It interacts with various proteins such as METTL3 an m6A methyltransferase which is vital for mediating m6A modification. It also interacts with YTH domain-containing proteins that recognize m6A marks influencing transcript dynamics and gene expression. The interplay of m6A with proteins in these pathways underlines its role in fine-tuning cellular processes and responses.

M6A modification has a significant impact on cancer and neurological disorders. In cancers alterations in m6A methylation patterns can promote oncogenic transformation and metastasis. m6A-related proteins such as FTO an m6A demethylase have shown connections to these pathways affecting cancer progression. In neurological disorders m6A impacts aspects of neural development and function and abnormalities in its regulation may contribute to diseases like Alzheimer's. Understanding the roles of m6A in diverse diseases can pave the way for novel therapeutic approaches.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information N6-methyladenosine (m6A)

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Publications (217)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules 15: PubMed40867632

2025

Apelin-13-Mediated Upregulation of METTL3 Ameliorates Alzheimer's Disease via Inhibiting Neuroinflammation Through m6A-Dependent Regulation of lncRNA BDNF-AS.

Applications

Unspecified application

Species

Unspecified reactive species

Li Han,Siwen Wei,Rong Wang,Yiran Liu,Yi Zhong,Juan Fu,Huaiqing Luo,Meihua Bao

Frontiers in pharmacology 16:1631076 PubMed40771928

2025

Ginsenoside Rb1 mitigates atherosclerosis in part through modulating FTO-mediated mA RNA modification in NETs-induced endothelial activation.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenni Yang,Minqi Xiong,Xinmiao Tang,Peiwei Wang,Jingang Cui,Yu Chen,Teng Zhang

Viruses 17: PubMed40573422

2025

Strain-Divergent m6A Landscapes Modulate Nipah Virus Replication and METTL3 Inhibition Attenuates Virulence.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Luo,Zhen Chen,Fang Zhang,Haibin Liu,Fang Huang,Xueyan Zhang,Jiangpeng Feng,Shuang Ding,Lishi Liu,Wuxiang Guan,Aiping Zeng,Haojie Hao

Nature communications 16:4930 PubMed40436842

2025

An RNA transmethylation pathway governs kidney nephrogenic potential.

Applications

Unspecified application

Species

Unspecified reactive species

Harini Ramalingam,Jesus Alvarez,Andrea Flaten,Patricia Cobo-Stark,Nicholas Foster,Elyse Grilli,Ronak Lakhia,Karam Aboudehen,Thomas Carroll,Vishal Patel

American journal of reproductive immunology (New York, N.Y. : 1989) 93:e70102 PubMed40435048

2025

Knockdown of TRIM47 Overcomes Paclitaxel Resistance in Ovarian Cancer by Suppressing the TGF-β Pathway via PPM1A.

Applications

Unspecified application

Species

Unspecified reactive species

Hezhu Wang,Shengjun Chen,Feifei Xu,Xiaojing Chen

Science advances 11:eadq3052 PubMed40435251

2025

FTO regulates ELK3-mediated metabolic rewiring and represents a unique therapeutic target in T cell leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Huang,Xinlu Li,Jinlian Luo,Chuan Gao,Mengjie Yang,Jin Xu,Ting Xie,Zhi Chen,Donghai Wang,Yuan Wang,Hua-Bing Li,Jinyan Huang,Yu Liu,Haojian Zhang,Panagiotis Ntziachristos,Yun Zhao,Guoliang Qing,Hudan Liu

Translational lung cancer research 14:1351-1370 PubMed40386722

2025

was stabilized through N-methyladenosine methylation and exerted its anticancer effects in lung squamous cell carcinoma by inhibiting SIRT1 activity via CCAR2.

Applications

Unspecified application

Species

Unspecified reactive species

Qihang Yan,Wingshing Wong,Jinsong Lei,Dachuan Liang,Jie Yang,Li Gong,Rossana Berardi,Shuqin Dai,Junye Wang

Molecular therapy. Nucleic acids 36:102533 PubMed40291376

2025

An inflammation-associated lncRNA induces neuronal damage via mitochondrial dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Ane Olazagoitia-Garmendia,Henar Rojas-Márquez,Tim Trobisch,Cristina Moreno-Castro,Ariadne Rodriguez Etxebarria,Jon Mentxaka,Ajai Tripathi,Bibo Yang,Itziar Martin Ruiz,Juan Anguita,J Javier Meana,Yiliang Ding,Ranjan Dutta,Lucas Schirmer,Mariana Igoillo-Esteve,Izortze Santin,Ainara Castellanos-Rubio

The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology 36:269-279 PubMed40241612

2025

Regulatory Role of IGF2BP2 in Intestinal Mucosal Barrier Dysfunction in Ulcerative Colitis.

Applications

Unspecified application

Species

Unspecified reactive species

Ruifan Li,Bin Gu,Anli Lv

Nature communications 16:2925 PubMed40133293

2025

FTO suppresses DNA repair by inhibiting PARP1.

Applications

Unspecified application

Species

Unspecified reactive species

Tianyi Zhu,Jing Zhi Anson Tan,Lingrui Zhang,He Huang,Sooraj S Das,Flora Cheng,Pranesh Padmanabhan,Mathew J K Jones,Mihwa Lee,Albert Lee,Jocelyn Widagdo,Victor Anggono

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-N6-methyladenosine (m6A) antibody

Nucleotide Array - Anti-N6-methyladenosine (m6A) antibody (AB151230)

Western blot - Anti-N6-methyladenosine (m6A) antibody (AB151230)

Key facts

Host species

Clonality

Isotype

Carrier free

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (217)

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