Anti-N6-methyladenosine (m6A) antibody is a rabbit polyclonal carrier-free antibody and is conjugation-ready. It is used in the N6-methyladenosine (m6A) nucleotide array. Suitable for modified nucleic acid samples.
- Cited in over 110 publications
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- JCI insight 8:2023Intestinal transit-amplifying cells require METTL3 for growth factor signaling and cell survival.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCharles H Danan,Kaitlyn E Naughton,Katharina E Hayer,Sangeevan Vellappan,Emily A McMillan,Yusen Zhou,Rina Matsuda,Shaneice K Nettleford,Kay Katada,Louis R Parham,Xianghui Ma,Afrah Chowdhury,Benjamin J Wilkins,Premal Shah,Matthew D Weitzman,Kathryn E HamiltonPubMed 37883185
- Scientific reports 13:207302023A1BG-AS1 promotes adriamycin resistance of breast cancer by recruiting IGF2BP2 to upregulate ABCB1 in an m6A-dependent manner.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJian Wang,Jie Xu,Jie ZhengPubMed 38007504
- Cell death discovery 9:3712023IGF2BP2 acts as a mA modification regulator in laryngeal squamous cell carcinoma through facilitating CDK6 mRNA stabilization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaojun Tang,Qinglai Tang,Shisheng Li,Mengmeng Li,Tao YangPubMed 37816718
- eNeuro 10:2023Upregulation of UBR1 m6A Methylation by METTL14 Inhibits Autophagy in Spinal Cord Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChangsheng Wang,Xitian Zhu,Rongsheng Chen,Xiaobo Zhang,Nancheng LianPubMed 37094938
- Journal of experimental & clinical cancer research : CR 42:1272023Epigenetically silenced lncRNA SNAI3-AS1 promotes ferroptosis in glioma via perturbing the mA-dependent recognition of Nrf2 mRNA mediated by SND1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianglin Zheng,Qing Zhang,Zhen Zhao,Yue Qiu,Yujie Zhou,Zhipeng Wu,Cheng Jiang,Xuan Wang,Xiaobing JiangPubMed 37202791
- Clinical and translational medicine 13:e12792023RNA demethylase ALKBH5 promotes colorectal cancer progression by posttranscriptional activation of RAB5A in an m6A-YTHDF2-dependent manner.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDingcheng Shen,Jinxin Lin,Yumo Xie,Zhuokai Zhuang,Gaopo Xu,Shaoyong Peng,Guannan Tang,Liangliang Bai,Mingxuan Zhu,Yu Zhang,Ziying Huang,Puning Wang,Xiaoxia Liu,Meijin Huang,Yanxin Luo,Xiaolin Wang,Huichuan YuPubMed 37203239
- eLife 12:2023Nuclear m6A reader YTHDC1 promotes muscle stem cell activation/proliferation by regulating mRNA splicing and nuclear export.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYulong Qiao,Qiang Sun,Xiaona Chen,Liangqiang He,Di Wang,Ruibao Su,Yuanchao Xue,Hao Sun,Huating WangPubMed 36892464
- World journal of surgical oncology 21:252023New insights on the interaction between mA modification and non-coding RNA in cervical squamous cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuqun Shen,Fen Li,Yan Wang,Yongmei Huang,Gulibiya Aizezi,Jinrui Yuan,Cailing Ma,Chen LinPubMed 36710350
- Proceedings of the National Academy of Sciences of the United States of America 120:e22103611202023PCIF1-mediated deposition of 5'-cap ,2'--dimethyladenosine in ACE2 and TMPRSS2 mRNA regulates susceptibility to SARS-CoV-2 infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLingling Wang,Shaobo Wang,Lujing Wu,Wanyu Li,William Bray,Alex E Clark,Gwendolyn Michelle Gonzalez,Yinsheng Wang,Aaron F Carlin,Tariq M RanaPubMed 36689652
- The EMBO journal 42:e1116732022Role of PCIF1-mediated 5'-cap N6-methyladeonsine mRNA methylation in colorectal cancer and anti-PD-1 immunotherapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLingling Wang,Lujing Wu,Zhouting Zhu,Qiong Zhang,Wanyu Li,Gwendolyn Michelle Gonzalez,Yinsheng Wang,Tariq M RanaPubMed 36514940
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| Nucleotide Array | |
|---|---|
| Modified Nucleic Acid | Tested |
Nucleotide Array
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Modified Nucleic Acid | Dilution info 2 µg/mL | Notes - |
Associated Products
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2 products for Alternative Product
Alternative names
N6-methyladenosine, m6A
Recommended products
Anti-N6-methyladenosine (m6A) antibody is a rabbit polyclonal carrier-free antibody and is conjugation-ready. It is used in the N6-methyladenosine (m6A) nucleotide array. Suitable for modified nucleic acid samples.
- Cited in over 110 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
N6-methyladenosine (m6A) is a post-transcriptional modification of RNA. m6A modification has been identified in all classes of RNA (rRNA, tRNA and mRNA) and is catalysed by an evolutionary conserved multi-subunit enzyme, methyltransferase like 3 (METTL3). Cellular and viral RNA has been known to be methylated for decades. Recent studies have found that mRNA is predominately m6A modified at stop codons and long internal exons, which are conserved between mouse and human. Emerging evidence suggests m6A plays an important role in regulating gene expression, alternative splicing patterns, downstream signalling (p53) as well as apoptosis.
The regulation of m6A modifications in mRNA has been linked to disease, where fat mass and obesity-associated (FTO) has been has been reported to be a obesity risk gene. FTO is a m6A demethylase and polymorphisms that result in increased FTO expression are associated with increased body mass and risk of obesity.
Product SpecificationsAnti-N6-methyladenosine (m6A) antibody (ab151230) is a rabbit polyclonal antibody and is validated for use in Nucleotide Array.
Anti-N6-methyladenosine (m6A) antibody (ab151230) specifically detects N6-methyladenosine (m6A) (UniProt ID: ; Molecular weight: kDa) and is sold in 100 µg selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-N6-methyladenosine (m6A) antibody (ab151230) has high sensitivity and specificity.
Anti-N6-methyladenosine (m6A) antibody (ab151230) has been cited over 111 times in peer reviewed journals and is trusted by the scientific community.
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Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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Terms & Conditions.
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Nucleotide Array - Anti-N6-methyladenosine (m6A) antibody (ab151230)
All batches of ab151230 are tested in Nucleotide Array against N6-methyladenosine (m6A) and unmodified adenosine. Six dilutions of each oligomer are printed on to the Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to N6-methyladenosine, indicating that this antibody specifically recognises adenosine methylated at position N6.
2Ome-m6A - N6-methyladenosine
2Ome-m6A_unmod - unmodified adenosine
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