AB286164

Anti-N6-methyladenosine (m6A) antibody

Name: Anti-N6-methyladenosine (m6A) antibody (ab286164) | Abcam
Brand: Abcam Limited
SKU: AB286164
Price: 490 USD
Availability: InStock

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(36 Publications)

Rabbit Polyclonal N6-methyladenosine (m6A) antibody. Suitable for WB and reacts with Modified Nucleic Acid samples. Cited in 36 publications.

View Alternative Names

N6-methyladenosine, m6A

1 Images

Supplier Data

Western blot - Anti-N6-methyladenosine (m6A) antibody (AB286164)

All lanes:

Western blot - Anti-N6-methyladenosine (m6A) antibody (ab286164)

Lane 1:

N6-Methyladenosine conjugated with BSA, 2 ng

Lane 2:

Adenosine conjugated with BSA, 2 ng

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The antibody is very specific for N6-Methyladenosine. It does not cross-react with adenosine as demonstrated by the Western blot.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Modified Nucleic Acid": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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Product details

This product is manufactured by BioVision, an Abcam company and was previously called 6653 N6-Methyladenosine Antibody. 6653-100 is the same size as the 100 μg size of ab286164.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Purified through a protein A column, followed by immunogen affinity purification

Storage buffer

pH: 7.2 Preservative: 0.03% Proclin 300 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

N6-methyladenosine (m6A) is a chemical modification present in RNA molecules specifically marking the adenosine base with a methyl group at the nitrogen-6 position. This modification weighing approximately 14 Da occurs in diverse eukaryotic species and influences RNA metabolism. m6A methylation can be detected using techniques like m6A dots blot or m6A ELISA. The modification is abundant in tissues like the brain and testis reflecting its critical role in varying cell types. Also m6A is known as a dynamic and reversible mark with its levels continuously adjusted by enzymes called writers (methyltransferases) erasers (demethylases) and readers (RNA-binding proteins).

Biological function summary

M6A methylation affects mRNA processing stability translation and decay. It integrates into large multi-protein complexes where it influences gene expression outcomes by affecting the RNA's interaction with the cellular machinery. This methylation modification acts as a regulatory signal that influences essential processes such as cell differentiation and circadian rhythms. Elucidating the biological functions of m6A involves studying how it affects RNA fate and its downstream gene regulatory networks.

Pathways

M6A modification is central to the mRNA metabolic pathway and the PI3K-Akt signaling pathway. It interacts with various proteins such as METTL3 an m6A methyltransferase which is vital for mediating m6A modification. It also interacts with YTH domain-containing proteins that recognize m6A marks influencing transcript dynamics and gene expression. The interplay of m6A with proteins in these pathways underlines its role in fine-tuning cellular processes and responses.

M6A modification has a significant impact on cancer and neurological disorders. In cancers alterations in m6A methylation patterns can promote oncogenic transformation and metastasis. m6A-related proteins such as FTO an m6A demethylase have shown connections to these pathways affecting cancer progression. In neurological disorders m6A impacts aspects of neural development and function and abnormalities in its regulation may contribute to diseases like Alzheimer's. Understanding the roles of m6A in diverse diseases can pave the way for novel therapeutic approaches.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information N6-methyladenosine (m6A)

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Publications (36)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 16:1630008 PubMed40808945

2025

Transcriptome-wide N6-methyladenosinem modifications analysis of chicken cecum in responding to inoculation.

Applications

Unspecified application

Species

Unspecified reactive species

Yanan Zhao,Yuanmei Wang,Liying Liu,Yanru Ren,Long Liu,Jiayi Wang,Xianyao Li

Oncology research 33:1679-1693 PubMed40612868

2025

N6-Methyladenosine Promotes the Transcription of c-Src Kinase via IRF1 to Facilitate the Proliferation of Liver Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yanxi Peng,Honggen Yuan,Zhanjie Jiang,Xiaoqing Ou,Qian Zhang,Kexin Yi,Yanbin Meng,Qun Xie

Mediators of inflammation 2025:6217272 PubMed40568348

2025

WTAP Accelerates Exhaustion of CD8 T Cells and Progression of Hepatocellular Carcinoma by Promoting m6A Modification and Translation of PD1 mRNA.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Li,Shunle Li,Hua Li,Bingli Liu,Zimu Wang,Huanqin Lei,Yuting Li,Lijuan Jia,Junhui Li,Hongwei Lu,Meng Xu

Cancer & metabolism 13:32 PubMed40533864

2025

NRF2 maintains redox balance via ME1 and NRF2 inhibitor synergizes with venetoclax in NPM1-mutated acute myeloid leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Jiayuan Hu,Zihao Yuan,Yan Shu,Jun Ren,Jing Yang,Lisha Tang,Xingyu Wei,Yongcan Liu,Fangfang Jin,Qiaoling Xiao,Xinyi Chen,Nan Wu,Wen Zhao,Ziwei Li,Ling Zhang

Cellular and molecular life sciences : CMLS 82:157 PubMed40220148

2025

m6A reader IGF2BP1 facilitates macrophage glycolytic metabolism and fibrotic phenotype by stabilizing THBS1 mRNA to promote pulmonary fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Hu,Lulu Yang,Liting Huang,Chao Zeng,Siying Ren

Cell death & disease 16:271 PubMed40210616

2025

Role of METTL16 in PPARγ methylation and osteogenic differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Liangjie Lu,Lijun Wang,Minjie Yang,Huihan Wang

Cells 14: PubMed40072093

2025

Modification of RNF183 via m6A Methylation Mediates Podocyte Dysfunction in Diabetic Nephropathy by Regulating PKM2 Ubiquitination and Degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Dongwei Guo,Yingxue Pang,Wenjie Wang,Yueying Feng,Luxuan Wang,Yuanyuan Sun,Jun Hao,Fan Li,Song Zhao

JACS Au 5:618-630 PubMed40017738

2025

Phenylpyrazoles as Inhibitors of the mA RNA-Binding Protein YTHDF2.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaqiu Qiu,Claus Kemker,Georg L Goebel,Philipp Lampe,Nadav Wallis,Damian Schiller,Katrin Bigler,Mao Jiang,Sonja Sievers,Gene W Yeo,Peng Wu

Cell communication and signaling : CCS 23:43 PubMed39849461

2025

O-GlcNAcylated FTO promotes m6A modification of SOX4 to enhance MDS/AML cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Junjie Gou,Jingjing Bi,Kexin Wang,Lei Lei,Yanli Feng,Zengqi Tan,Jiaojiao Gao,Yanan Song,Enci Kang,Feng Guan,Xiang Li

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 38:e70154 PubMed39565355

2024

METTL3 and IGF2BP2 coordinately regulate FOSL1 mRNA via m6A modification, suppressing trophoblast invasion and contributing to fetal growth restriction.

Applications

Unspecified application

Species

Unspecified reactive species

Ruixin Chen,Tingting Wang,Hai Tong,Xue Zhang,Jie Ruan,Hongbo Qi,Xinghui Liu,Guolin He

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-N6-methyladenosine (m6A) antibody

Western blot - Anti-N6-methyladenosine (m6A) antibody (AB286164)

Key facts

Host species

Clonality

Isotype

Carrier free

Applications

Immunogen

Specificity

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Assay Kits

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (36)

Product promise