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AB284136

Anti-N6-methyladenosine (m6A) antibody [EPR23561-164] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal N6-methyladenosine (m6A) antibody. Carrier free. Suitable for Dot, FRET and reacts with Modified Nucleic Acid samples. Cited in 1 publication.

View Alternative Names

N6-methyladenosine, m6A

3 Images
Dot Blot - Anti-N6-methyladenosine (m6A) antibody [EPR23561-164] - BSA and Azide free (AB284136)
  • Dot

Supplier Data

Dot Blot - Anti-N6-methyladenosine (m6A) antibody [EPR23561-164] - BSA and Azide free (AB284136)

This data was developed using ab284130, the same antibody clone in a different buffer formulation.

Dot blot analysis of N6-methyladenosine (m6A) using ab284130 at 1/5000 (0.0001μg/mL) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20,000 dilution.

Lane 1 : HeLa total RNA

Lane 2 : HeLa mRNA

Exposure time : 20 seconds

Blocking and diluting buffer and concentration : 5% NFDM/TBST

FRET - Anti-N6-methyladenosine (m6A) antibody [EPR23561-164] - BSA and Azide free (AB284136)
  • FRET

Supplier Data

FRET - Anti-N6-methyladenosine (m6A) antibody [EPR23561-164] - BSA and Azide free (AB284136)

This data was developed using ab284130, the same antibody clone in a different buffer formulation.

HTRF was performed on 25 nm of each biotinylated oligos and using ab284130 at a concentration range of 0.001-4.000 ug/ml. Species-specific Terbium cryptate- labelled anti-rabbit IgG antibody and D2-labeled Streptavidin were used.

Dot Blot - Anti-N6-methyladenosine (m6A) antibody [EPR23561-164] - BSA and Azide free (AB284136)
  • Dot

Supplier Data

Dot Blot - Anti-N6-methyladenosine (m6A) antibody [EPR23561-164] - BSA and Azide free (AB284136)

This data was developed using ab284130, the same antibody clone in a different buffer formulation.

Dot blot analysis of N6-methyladenosine (m6A) using ab284130 at 1/5000 (0.0001μg/mL) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20,000 dilution.

Lane 1 : m6, 6A-oligo

Lane 2 : A-oligo

Lane 3 : Am-oligo

Lane 4 : m6A-oligo

Lane 5 : m6Am-oligo

Exposure time : 6 seconds

Blocking and diluting buffer and concentration : 5% NFDM/TBST

  • Unconjugated

    Anti-N6-methyladenosine (m6A) antibody [EPR23561-164]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23561-164

Isotype

IgG

Carrier free

Yes

Applications

FRET, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Anti-N6-methyladenosine (m6A) antibody [EPR23561-164]

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Primary Antibodies

AB190886

Anti-N6-methyladenosine (m6A) antibody [mAbcam 190886] - BSA and Azide free

RabMAb|Recombinant

Nucleotide Array - Anti-N6-methyladenosine (m6A) antibody [mAbcam 190886] - BSA and Azide free (AB190886)

  • 4
  • 2
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "FRET" : {"fullname" : "FRET", "shortname":"FRET"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Modified Nucleic Acid": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "FRET-species-checked": "testedAndGuaranteed", "FRET-species-dilution-info": "0.001-4 µg/mL", "FRET-species-notes": "<p>HTRF readout</p>" } } }
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Product details

ab284136 the carrier-free version of ab284130.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

N6-methyladenosine (m6A) is a chemical modification present in RNA molecules specifically marking the adenosine base with a methyl group at the nitrogen-6 position. This modification weighing approximately 14 Da occurs in diverse eukaryotic species and influences RNA metabolism. m6A methylation can be detected using techniques like m6A dots blot or m6A ELISA. The modification is abundant in tissues like the brain and testis reflecting its critical role in varying cell types. Also m6A is known as a dynamic and reversible mark with its levels continuously adjusted by enzymes called writers (methyltransferases) erasers (demethylases) and readers (RNA-binding proteins).
Biological function summary

M6A methylation affects mRNA processing stability translation and decay. It integrates into large multi-protein complexes where it influences gene expression outcomes by affecting the RNA's interaction with the cellular machinery. This methylation modification acts as a regulatory signal that influences essential processes such as cell differentiation and circadian rhythms. Elucidating the biological functions of m6A involves studying how it affects RNA fate and its downstream gene regulatory networks.

Pathways

M6A modification is central to the mRNA metabolic pathway and the PI3K-Akt signaling pathway. It interacts with various proteins such as METTL3 an m6A methyltransferase which is vital for mediating m6A modification. It also interacts with YTH domain-containing proteins that recognize m6A marks influencing transcript dynamics and gene expression. The interplay of m6A with proteins in these pathways underlines its role in fine-tuning cellular processes and responses.

M6A modification has a significant impact on cancer and neurological disorders. In cancers alterations in m6A methylation patterns can promote oncogenic transformation and metastasis. m6A-related proteins such as FTO an m6A demethylase have shown connections to these pathways affecting cancer progression. In neurological disorders m6A impacts aspects of neural development and function and abnormalities in its regulation may contribute to diseases like Alzheimer's. Understanding the roles of m6A in diverse diseases can pave the way for novel therapeutic approaches.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information N6-methyladenosine (m6A)
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Drug development research 86:e70065 PubMed39950329

2025

Exosomal ALKBH5 Alleviates Vascular Calcification by Suppressing Cell Apoptosis via m6A-Modified GSDME.

Applications

Unspecified application

Species

Unspecified reactive species

Guian Xu,Qingman Li,Lijie Zhu,Tingjie Yang,Yapan Yang,Honghui Yang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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