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AB314476

Anti-N6-methyladenosine (m6A) antibody [EPXVJ-1-2]

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(2 Publications)

Rabbit Recombinant Monoclonal N6-methyladenosine (m6A) antibody. Suitable for FRET, Dot and reacts with Nucleic Acid samples. Cited in 2 publications.
3 Images
Dot Blot - Anti-N6-methyladenosine (m6A) antibody [EPXVJ-1-2] (AB314476)
  • Dot

Supplier Data

Dot Blot - Anti-N6-methyladenosine (m6A) antibody [EPXVJ-1-2] (AB314476)

Dot blot analysis of N6-methyladenosine (m6A) using ab314476 at 1 : 5000 (0.106 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 20,000 dilution. Lane 1 : Hela (human cervical adenocarcinoma epithelial cell) total RNA Lane 2 : Hela (human cervical adenocarcinoma epithelial cell) mRNA Exposure time : 200s Blocking and diluting buffer and concentration : 5% NFDM/TBST

FRET - Anti-N6-methyladenosine (m6A) antibody [EPXVJ-1-2] (AB314476)
  • FRET

Supplier Data

FRET - Anti-N6-methyladenosine (m6A) antibody [EPXVJ-1-2] (AB314476)

A FRET-based assay was performed using 25 nm of each biotinylated oligo and ab314476 at a concentration range of 0.001-4.000 ug/ml. The Secondary antibody used was SA_D2 binding to antigen, GAR_tb binding to antibody at 1 : 100 dilution. Antigen : m6A RNA oligo, m6Am RNA oligo, Am RNA oligo, m6, 6A RNA oligo, m1A RNA oligo, m8A RNA oligo, m1G RNA oligo, m2G RNA oligo, A RNA oligo. Antigen concentration : 100 nmol/ml

Dot Blot - Anti-N6-methyladenosine (m6A) antibody [EPXVJ-1-2] (AB314476)
  • Dot

Supplier Data

Dot Blot - Anti-N6-methyladenosine (m6A) antibody [EPXVJ-1-2] (AB314476)

Dot blot analysis of N6-methyladenosine (m6A) using ab314476 at 1 : 5000 (0.106 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 20,000 dilution. Lane 1 : A-oligo Lane 2 : m6A-oligo Lane 3 : m6Am-oligo Lane 4 : Am-oligo Lane 5 : m6, 6A-oligo Lane 6 : m1A-oligo Lane 7 : m8A-oligo Lane 8 : m1G-oligo Lane 9 : m2G-oligo Exposure time : 5s Blocking and diluting buffer and concentration : 5% NFDM/TBST

  • Carrier free

    Anti-N6-methyladenosine (m6A) antibody [EPXVJ-1-2] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPXVJ-1-2

Isotype

IgG

Carrier free

No

Applications

FRET, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody targets both m6A and m6Am as seen in the FRET-based assay and Dot blot data, with a possible stronger preference for m6Am.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FRET" : {"fullname" : "FRET", "shortname":"FRET"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Nucleic Acid": { "FRET-species-checked": "testedAndGuaranteed", "FRET-species-dilution-info": "0.001-4 µg/mL", "FRET-species-notes": "<p></p>", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/5000", "Dot-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

N6-methyladenosine (m6A) is a chemical modification present in RNA molecules specifically marking the adenosine base with a methyl group at the nitrogen-6 position. This modification weighing approximately 14 Da occurs in diverse eukaryotic species and influences RNA metabolism. m6A methylation can be detected using techniques like m6A dots blot or m6A ELISA. The modification is abundant in tissues like the brain and testis reflecting its critical role in varying cell types. Also m6A is known as a dynamic and reversible mark with its levels continuously adjusted by enzymes called writers (methyltransferases) erasers (demethylases) and readers (RNA-binding proteins).
Biological function summary

M6A methylation affects mRNA processing stability translation and decay. It integrates into large multi-protein complexes where it influences gene expression outcomes by affecting the RNA's interaction with the cellular machinery. This methylation modification acts as a regulatory signal that influences essential processes such as cell differentiation and circadian rhythms. Elucidating the biological functions of m6A involves studying how it affects RNA fate and its downstream gene regulatory networks.

Pathways

M6A modification is central to the mRNA metabolic pathway and the PI3K-Akt signaling pathway. It interacts with various proteins such as METTL3 an m6A methyltransferase which is vital for mediating m6A modification. It also interacts with YTH domain-containing proteins that recognize m6A marks influencing transcript dynamics and gene expression. The interplay of m6A with proteins in these pathways underlines its role in fine-tuning cellular processes and responses.

M6A modification has a significant impact on cancer and neurological disorders. In cancers alterations in m6A methylation patterns can promote oncogenic transformation and metastasis. m6A-related proteins such as FTO an m6A demethylase have shown connections to these pathways affecting cancer progression. In neurological disorders m6A impacts aspects of neural development and function and abnormalities in its regulation may contribute to diseases like Alzheimer's. Understanding the roles of m6A in diverse diseases can pave the way for novel therapeutic approaches.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information N6-methyladenosine (m6A)
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell & bioscience 15:61 PubMed40380235

2025

The HNRNPC/CELF2 signaling pathway drives glycolytic reprogramming and mitochondrial dysfunction in drug-resistant acute myeloid leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Ma,Haodong Li,Ziqi Zhao,Changchun Li,Man Wang,Lele Zhang,Yutong Zhao,Haipeng Su,Feng Wang,Jiai Hua

Clinics (Sao Paulo, Brazil) 80:100658 PubMed40245789

2025

Kynurenine promotes the immune escape of colorectal cancer cells via NAT10-mediated acC acetylation of PD-L1.

Applications

Unspecified application

Species

Unspecified reactive species

Zaibiao Wang,Manman Yin,Ruhang Zhou,Ming Li,Jie Peng,Zhengguang Wang
View all publications
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Product promise

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