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AB314477

Anti-N6-methyladenosine (m6A) antibody [EPXVJ-1-2] - BSA and Azide free

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Rabbit Recombinant Monoclonal N6-methyladenosine (m6A) antibody. Carrier free. Suitable for FRET, Dot and reacts with Nucleic Acid samples.

View Alternative Names

N6-methyladenosine, m6A

3 Images
Dot Blot - Anti-N6-methyladenosine (m6A) antibody [EPXVJ-1-2] - BSA and Azide free (AB314477)
  • Dot

Supplier Data

Dot Blot - Anti-N6-methyladenosine (m6A) antibody [EPXVJ-1-2] - BSA and Azide free (AB314477)

This data was developed using ab314476, the same antibody clone in a different buffer formulation. Dot blot analysis of N6-methyladenosine (m6A) using ab314476 at 1 : 5000 (0.106 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 20,000 dilution. Lane 1 : Hela (human cervical adenocarcinoma epithelial cell) total RNA Lane 2 : Hela (human cervical adenocarcinoma epithelial cell) mRNA Exposure time : 200s Blocking and diluting buffer and concentration : 5% NFDM/TBST

FRET - Anti-N6-methyladenosine (m6A) antibody [EPXVJ-1-2] - BSA and Azide free (AB314477)
  • FRET

Supplier Data

FRET - Anti-N6-methyladenosine (m6A) antibody [EPXVJ-1-2] - BSA and Azide free (AB314477)

This data was developed using ab314476, the same antibody clone in a different buffer formulation. A FRET-based assay was performed using 25 nm of each biotinylated oligo and ab314476 at a concentration range of 0.001-4.000 ug/ml. The Secondary antibody used was SA_D2 binding to antigen, GAR_tb binding to antibody at 1 : 100 dilution. Antigen : m6A RNA oligo, m6Am RNA oligo, Am RNA oligo, m6, 6A RNA oligo, m1A RNA oligo, m8A RNA oligo, m1G RNA oligo, m2G RNA oligo, A RNA oligo. Antigen concentration : 100 nmol/ml

Dot Blot - Anti-N6-methyladenosine (m6A) antibody [EPXVJ-1-2] - BSA and Azide free (AB314477)
  • Dot

Supplier Data

Dot Blot - Anti-N6-methyladenosine (m6A) antibody [EPXVJ-1-2] - BSA and Azide free (AB314477)

This data was developed using ab314476, the same antibody clone in a different buffer formulation. Dot blot analysis of N6-methyladenosine (m6A) using ab314476 at 1 : 5000 (0.106 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 20,000 dilution. Lane 1 : A-oligo Lane 2 : m6A-oligo Lane 3 : m6Am-oligo Lane 4 : Am-oligo Lane 5 : m6, 6A-oligo Lane 6 : m1A-oligo Lane 7 : m8A-oligo Lane 8 : m1G-oligo Lane 9 : m2G-oligo Exposure time : 5s Blocking and diluting buffer and concentration : 5% NFDM/TBST

  • Unconjugated

    Anti-N6-methyladenosine (m6A) antibody [EPXVJ-1-2]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPXVJ-1-2

Isotype

IgG

Carrier free

Yes

Applications

Dot, FRET

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody targets both m6A and m6Am as seen in the FRET-based assay and Dot blot data, with a possible stronger preference for m6Am.

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Complementary Products

Assay Kits

AB185912

m6A RNA Methylation Assay Kit (Colorimetric)

ELISA - m6A RNA Methylation Assay Kit (Colorimetric) (AB185912)

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Reactivity data

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Product details

ab314477 is the carrier-free version of ab314476.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

N6-methyladenosine (m6A) is a chemical modification present in RNA molecules specifically marking the adenosine base with a methyl group at the nitrogen-6 position. This modification weighing approximately 14 Da occurs in diverse eukaryotic species and influences RNA metabolism. m6A methylation can be detected using techniques like m6A dots blot or m6A ELISA. The modification is abundant in tissues like the brain and testis reflecting its critical role in varying cell types. Also m6A is known as a dynamic and reversible mark with its levels continuously adjusted by enzymes called writers (methyltransferases) erasers (demethylases) and readers (RNA-binding proteins).
Biological function summary

M6A methylation affects mRNA processing stability translation and decay. It integrates into large multi-protein complexes where it influences gene expression outcomes by affecting the RNA's interaction with the cellular machinery. This methylation modification acts as a regulatory signal that influences essential processes such as cell differentiation and circadian rhythms. Elucidating the biological functions of m6A involves studying how it affects RNA fate and its downstream gene regulatory networks.

Pathways

M6A modification is central to the mRNA metabolic pathway and the PI3K-Akt signaling pathway. It interacts with various proteins such as METTL3 an m6A methyltransferase which is vital for mediating m6A modification. It also interacts with YTH domain-containing proteins that recognize m6A marks influencing transcript dynamics and gene expression. The interplay of m6A with proteins in these pathways underlines its role in fine-tuning cellular processes and responses.

M6A modification has a significant impact on cancer and neurological disorders. In cancers alterations in m6A methylation patterns can promote oncogenic transformation and metastasis. m6A-related proteins such as FTO an m6A demethylase have shown connections to these pathways affecting cancer progression. In neurological disorders m6A impacts aspects of neural development and function and abnormalities in its regulation may contribute to diseases like Alzheimer's. Understanding the roles of m6A in diverse diseases can pave the way for novel therapeutic approaches.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information N6-methyladenosine (m6A)
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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