AB190886

Anti-N6-methyladenosine (m6A) antibody [mAbcam 190886] - BSA and Azide free

Name: Anti-N6-methyladenosine (m6A) antibody [mAbcam 190886] - BSA and Azide free (ab190886) | Abcam
Brand: Abcam Limited
SKU: AB190886
Price: 905 USD
Availability: InStock
Rating: 4 (2 reviews)

RabMAb
Recombinant
What is this?

(2 Reviews)

(15 Publications)

Rabbit Recombinant Monoclonal N6-methyladenosine (m6A) antibody. Carrier free. Suitable for Nucleotide Array and reacts with Modified Nucleic Acid samples. Cited in 15 publications.

View Alternative Names

N6-methyladenosine, m6A

1 Images

Nucleotide Array

Lab

Nucleotide Array - Anti-N6-methyladenosine (m6A) antibody [mAbcam 190886] - BSA and Azide free (AB190886)

All batches of ab190886 are tested in Nucleotide Array against N6-methyladenosine and unmodified adenosine. Six dilutions of each oligomer are printed on to the Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to N6-methyladenosine, indicating that this antibody specifically recognises adenosine methylated at position N6.

2Ome-m6A - N6-methyladenosine

2Ome-m6A_unmod - unmodified adenosine

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

mAbcam 190886

Isotype

IgG

Carrier free

Yes

Applications

Nucleotide Array

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "NucleotideArray" : {"fullname" : "Nucleotide Array", "shortname":"Nucleotide Array"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Modified Nucleic Acid": { "NucleotideArray-species-checked": "testedAndGuaranteed", "NucleotideArray-species-dilution-info": "2 µg/mL", "NucleotideArray-species-notes": "<p></p>" } } }

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Product details

N6-methyladenosine (m6A) is a post-transcriptional modification of RNA. m6A modification has been identified in all classes of RNA (rRNA, tRNA and mRNA) and is catalysed by an evolutionary conserved multi-subunit enzyme, methyltransferase like 3 (METTL3). Cellular and viral RNA has been known to be methylated for decades. Recent studies have found that mRNA is predominately m6A modified at stop codons and long internal exons, which are conserved between mouse and human. Emerging evidence suggests m6A plays an important role in regulating gene expression, alternative splicing patterns, downstream signalling (p53) as well as apoptosis.

The regulation of m6A modifications in mRNA has been linked to disease, where fat mass and obesity-associated (FTO) has been has been reported to be a obesity risk gene. FTO is a m6A demethylase and polymorphisms that result in increased FTO expression are associated with increased body mass and risk of obesity.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

N6-methyladenosine (m6A) is a chemical modification present in RNA molecules specifically marking the adenosine base with a methyl group at the nitrogen-6 position. This modification weighing approximately 14 Da occurs in diverse eukaryotic species and influences RNA metabolism. m6A methylation can be detected using techniques like m6A dots blot or m6A ELISA. The modification is abundant in tissues like the brain and testis reflecting its critical role in varying cell types. Also m6A is known as a dynamic and reversible mark with its levels continuously adjusted by enzymes called writers (methyltransferases) erasers (demethylases) and readers (RNA-binding proteins).

Biological function summary

M6A methylation affects mRNA processing stability translation and decay. It integrates into large multi-protein complexes where it influences gene expression outcomes by affecting the RNA's interaction with the cellular machinery. This methylation modification acts as a regulatory signal that influences essential processes such as cell differentiation and circadian rhythms. Elucidating the biological functions of m6A involves studying how it affects RNA fate and its downstream gene regulatory networks.

Pathways

M6A modification is central to the mRNA metabolic pathway and the PI3K-Akt signaling pathway. It interacts with various proteins such as METTL3 an m6A methyltransferase which is vital for mediating m6A modification. It also interacts with YTH domain-containing proteins that recognize m6A marks influencing transcript dynamics and gene expression. The interplay of m6A with proteins in these pathways underlines its role in fine-tuning cellular processes and responses.

M6A modification has a significant impact on cancer and neurological disorders. In cancers alterations in m6A methylation patterns can promote oncogenic transformation and metastasis. m6A-related proteins such as FTO an m6A demethylase have shown connections to these pathways affecting cancer progression. In neurological disorders m6A impacts aspects of neural development and function and abnormalities in its regulation may contribute to diseases like Alzheimer's. Understanding the roles of m6A in diverse diseases can pave the way for novel therapeutic approaches.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information N6-methyladenosine (m6A)

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular physiology 238:2361-2372 PubMed37565545

2023

Mettl14-mediated m A modification regulates the abnormal differentiation of small intestinal epithelial stem cells in diabetic state.

Applications

Unspecified application

Species

Unspecified reactive species

Ti-Dong Shan,Yue Han,Ming-Quan Song,Li Chen

Neuropathology and applied neurobiology 49:e12885 PubMed36709989

2023

m A mRNA methylation in human brain is disrupted in Lewy body disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Braulio Martinez De La Cruz,Chris Gell,Robert Markus,Ian Macdonald,Rupert Fray,Helen Miranda Knight

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 36:e22392 PubMed35716070

2022

Mettl3-mediated m A modification of Lrp2 facilitates neurogenesis through Ythdc2 and elicits antidepressant-like effects.

Applications

Unspecified application

Species

Unspecified reactive species

Biao Xu,Qingfeng Li,Yuanfei Wu,Huan Wang,Jiamin Xu,Hui Liu,Aiguo Xuan

Cell death discovery 8:143 PubMed35354789

2022

METTL14 promotes prostate tumorigenesis by inhibiting THBS1 via an m6A-YTHDF2-dependent mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Yongjie Wang,Junfei Chen,Wei-Qiang Gao,Ru Yang

Molecular therapy. Nucleic acids 27:547-561 PubMed35036065

2022

METTL14-mediated Lnc-LSG1 m6A modification inhibits clear cell renal cell carcinoma metastasis via regulating ESRP2 ubiquitination.

Applications

Unspecified application

Species

Unspecified reactive species

Danyang Shen,Lifeng Ding,Zeyi Lu,Ruyue Wang,Chenhao Yu,Huan Wang,Qiming Zheng,Xuliang Wang,Wanjiang Xu,Haifeng Yu,Liwei Xu,Mingchao Wang,Shicheng Yu,Shibin Zhu,Jun Qian,Liqun Xia,Gonghui Li

Cell biology international 46:359-369 PubMed34865263

2021

METTL3 is a key regulator of milk synthesis in mammary epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lulu Wang,Hao Qi,Dong Li,Lijie Liu,Dongying Chen,Xuejun Gao

Molecular psychiatry 26:7141-7153 PubMed34663904

2021

Modifying the mA brain methylome by ALKBH5-mediated demethylation: a new contender for synaptic tagging.

Applications

Unspecified application

Species

Unspecified reactive species

Braulio Martinez De La Cruz,Robert Markus,Sunir Malla,Maria Isabel Haig,Chris Gell,Fei Sang,Eleanor Bellows,Mahmoud Awad Sherif,Denise McLean,Anbarasu Lourdusamy,Tim Self,Zsuzsanna Bodi,Stuart Smith,Michael Fay,Ian A Macdonald,Rupert Fray,Helen Miranda Knight

Nature methods 18:1213-1222 PubMed34594034

2021

Systematic calibration of epitranscriptomic maps using a synthetic modification-free RNA library.

Applications

Unspecified application

Species

Unspecified reactive species

Zhang Zhang,Tao Chen,Hong-Xuan Chen,Ying-Yuan Xie,Li-Qian Chen,Yu-Li Zhao,Biao-Di Liu,Lingmei Jin,Wutong Zhang,Chang Liu,Dong-Zhao Ma,Guo-Shi Chai,Ying Zhang,Wen-Shuo Zhao,Wen Hui Ng,Jiekai Chen,Guifang Jia,Jianhua Yang,Guan-Zheng Luo

Nucleic acids research 49:5568-5587 PubMed33999208

2021

m6A RNA methylation of major satellite repeat transcripts facilitates chromatin association and RNA:DNA hybrid formation in mouse heterochromatin.

Applications

Unspecified application

Species

Unspecified reactive species

Katarzyna J Duda,Reagan W Ching,Lisa Jerabek,Nicholas Shukeir,Galina Erikson,Bettina Engist,Megumi Onishi-Seebacher,Valentina Perrera,Florian Richter,Gerhard Mittler,Katharina Fritz,Mark Helm,Philip Knuckles,Marc Bühler,Thomas Jenuwein

Theriogenology 158:309-320 PubMed33007716

2020

Ascorbic acid promotes the reproductive function of porcine immature Sertoli cells through transcriptome reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Wei Yang,Lu Chen,Qiao Mou,Hao Liang,Zhi-Qiang Du,Cai-Xia Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-N6-methyladenosine (m6A) antibody [mAbcam 190886] - BSA and Azide free

Nucleotide Array - Anti-N6-methyladenosine (m6A) antibody [mAbcam 190886] - BSA and Azide free (AB190886)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (15)

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