Rabbit Polyclonal NAA38 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NAA38.
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LSMD1, MAK31, PFAAP2, NAA38, LSM domain-containing protein 1, Phosphonoformate immuno-associated protein 2
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAA38 antibody (AB236965)
Paraffin-embedded human kidney tissue stained for NAA38 using ab236965 at 1/400 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
- ICC/IF
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Immunocytochemistry/ Immunofluorescence - Anti-NAA38 antibody (AB236965)
PC-3 (human prostate adenocarcinoma cell line) cells stained for NAA38 (Green) using ab236965 at 1/133 dilution in ICC/IF, followed by Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody.
The cells were fixed in 4% formaldehyde, permeabilized tissue using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C. Counterstained with DAPI.
- WB
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Western blot - Anti-NAA38 antibody (AB236965)
All lanes:
Western blot - Anti-NAA38 antibody (ab236965) at 1/500 dilution
All lanes:
Mouse kidney tissue lysate
Secondary
All lanes:
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 14 kDa
false
Reactivity data
Properties and storage information
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Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
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Aliquoting information
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Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
NAA38 functions within the NatC complex where it catalyzes N-terminal acetylation of a subset of proteins. This modification can regulate protein stability localization and interactions. NAA38 activity influences numerous cellular processes such as cell cycle regulation and apoptosis highlighting its role in maintaining cellular homeostasis. As part of the NatC complex NAA38 works synergistically with other complex components like NAA35 and NAA15 to achieve its biological functions.
Pathways
The acetylation process involving NAA38 integrates into the broader protein modification pathway influencing key cellular mechanisms. It directly interacts through acetylation with proteins in the cell cycle and apoptosis pathways controlling cellular proliferation and programmed cell death. This complex modulation assists in regulating cellular responses to external signals and the protein interacts with pathway components such as Cyclin-Dependent Kinase (CDK) and Bcl-xL playing a regulatory role in these pathways.
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Target data
Publications (1)
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Frontiers in endocrinology 14:1129930 PubMed37065733
2023
Applications
Unspecified application
Species
Unspecified reactive species
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