Rabbit Polyclonal NAB1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human NGFI-A-binding protein 1 aa 450 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human NGFI-A-binding protein 1 aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q13506
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Rat | Predicted | Predicted |
| Syrian hamster | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-5.00000 µg/mg of lysate | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Syrian hamster | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Syrian hamster | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2.
Alternative names
NGFI-A-binding protein 1, EGR-1-binding protein 1, Transcriptional regulatory protein p54, NAB1
Recommended products
Rabbit Polyclonal NAB1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human NGFI-A-binding protein 1 aa 450 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human NGFI-A-binding protein 1 aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q13506
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab241305 was affinity purified using an epitope specific to NAB1 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NAB1 also known as NGFI-A-binding protein 1 is a transcriptional repressor that interacts with zinc finger proteins to regulate gene expression. NAB1 has a molecular mass of approximately 66 kDa. This protein is expressed in various tissues including the nervous system. It plays a role in modulating the activity of target genes by binding to specific DNA sequences in cooperation with EGR transcription factors.
- Biological function summary
NAB1 functions as a critical regulator in cellular processes by forming a complex with EGR-1 also known as early growth response protein 1. This interaction allows NAB1 to influence the expression of several genes involved in cell differentiation proliferation and apoptosis. NAB1 ensures proper cellular responses to environmental stimuli by repressing gene transcription that EGR-1 activates.
- Pathways
NAB1 frequently associates with the MAPK/ERK signaling pathway and the apoptotic pathway. In the MAPK/ERK pathway it operates alongside proteins like EGR-1 to influence cellular proliferation and differentiation. Meanwhile NAB1's role in the apoptotic pathway is related to its interaction with genes and proteins regulating cell death enabling cells to respond appropriately to stress signals.
- Associated diseases and disorders
NAB1 has been linked to conditions like cancer and neurodegenerative diseases. Aberrant expression or dysfunction of NAB1 along with changes in other proteins such as EGR-1 can lead to uncontrolled cell growth and transformation contributing to tumorigenesis. Additionally misregulation of NAB1 may play a part in neurodegenerative disorders by affecting neuron survival and death though further research is needed to fully understand these connections.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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2 product images
Immunoprecipitation - Anti-NAB1 antibody (ab241305)
NAB1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) usind ab241305 at 6 μg/mg lysate. Western bot was performed on the immunoprecipitate using ab241305 at 0.4 μg/ml.
Lane 1: ab241305 IP in HeLa whole cell lysate.
Lane 2: Control IgG.Detection: Chemiluminescence with an exposure time of 10 seconds.
All lanes: Immunoprecipitation - Anti-NAB1 antibody (ab241305)
Predicted band size: 54 kDa
Western blot - Anti-NAB1 antibody (ab241305)
All lanes: Western blot - Anti-NAB1 antibody (ab241305) at 0.04 µg/mL
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 15 µg
Lane 3: HeLa whole cell lysate at 5 µg
Lane 4: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 5: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 54 kDa
Exposure time: 3min
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