AB241305

Anti-NAB1 antibody

Name: Anti-NAB1 antibody (ab241305) | Abcam
Brand: Abcam Limited
SKU: AB241305

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(1 Publication)

Rabbit Polyclonal NAB1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human NGFI-A-binding protein 1 aa 450 to C-terminus.

View Alternative Names

NGFI-A-binding protein 1, EGR-1-binding protein 1, Transcriptional regulatory protein p54, NAB1

2 Images

Supplier Data

Immunoprecipitation - Anti-NAB1 antibody (AB241305)

NAB1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) usind ab241305 at 6 μg/mg lysate. Western bot was performed on the immunoprecipitate using ab241305 at 0.4 μg/ml.

Lane 1 : ab241305 IP in HeLa whole cell lysate.
Lane 2 : Control IgG.

Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-NAB1 antibody (ab241305)

Predicted band size: 54 kDa

false

Supplier Data

Western blot - Anti-NAB1 antibody (AB241305)

All lanes:

Western blot - Anti-NAB1 antibody (ab241305) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 54 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human NGFI-A-binding protein 1 aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q13506

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Syrian hamster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab241305 was affinity purified using an epitope specific to NAB1 immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NAB1 also known as NGFI-A-binding protein 1 is a transcriptional repressor that interacts with zinc finger proteins to regulate gene expression. NAB1 has a molecular mass of approximately 66 kDa. This protein is expressed in various tissues including the nervous system. It plays a role in modulating the activity of target genes by binding to specific DNA sequences in cooperation with EGR transcription factors.

Biological function summary

NAB1 functions as a critical regulator in cellular processes by forming a complex with EGR-1 also known as early growth response protein 1. This interaction allows NAB1 to influence the expression of several genes involved in cell differentiation proliferation and apoptosis. NAB1 ensures proper cellular responses to environmental stimuli by repressing gene transcription that EGR-1 activates.

Pathways

NAB1 frequently associates with the MAPK/ERK signaling pathway and the apoptotic pathway. In the MAPK/ERK pathway it operates alongside proteins like EGR-1 to influence cellular proliferation and differentiation. Meanwhile NAB1's role in the apoptotic pathway is related to its interaction with genes and proteins regulating cell death enabling cells to respond appropriately to stress signals.

NAB1 has been linked to conditions like cancer and neurodegenerative diseases. Aberrant expression or dysfunction of NAB1 along with changes in other proteins such as EGR-1 can lead to uncontrolled cell growth and transformation contributing to tumorigenesis. Additionally misregulation of NAB1 may play a part in neurodegenerative disorders by affecting neuron survival and death though further research is needed to fully understand these connections.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2.

See full target information NGFI-A-binding protein 1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology reports 43:1309-1318 PubMed32323796

2020

Oroxylin A exerts anticancer effects on human ovarian cancer cells via the PPARγ‑dependent reversal of the progesterone receptor membrane component 1/2 expression profile.

Applications

Unspecified application

Species

Unspecified reactive species

Jian-Jiang Shen,Xiao-Fei Zhu,Juan Xu,Zhi-Fei Wang,Wan-Jian Gu,Yun Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NAB1 antibody

Immunoprecipitation - Anti-NAB1 antibody (AB241305)

Western blot - Anti-NAB1 antibody (AB241305)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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