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AB150389

Anti-NAB1 antibody [EPR8227(2)]

1

(1 Review)

|

(2 Publications)

Rabbit Recombinant Monoclonal NAB1 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

NGFI-A-binding protein 1, EGR-1-binding protein 1, Transcriptional regulatory protein p54, NAB1

1 Images
Western blot - Anti-NAB1 antibody [EPR8227(2)] (AB150389)
  • WB

Unknown

Western blot - Anti-NAB1 antibody [EPR8227(2)] (AB150389)

All lanes:

Western blot - Anti-NAB1 antibody [EPR8227(2)] (ab150389) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

U87 MG cell lysate at 10 µg

Lane 4:

293T cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit antibody at 1/2000 dilution

Predicted band size: 54 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8227(2)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NAB1 also known as NGFI-A-binding protein 1 is a transcriptional repressor that interacts with zinc finger proteins to regulate gene expression. NAB1 has a molecular mass of approximately 66 kDa. This protein is expressed in various tissues including the nervous system. It plays a role in modulating the activity of target genes by binding to specific DNA sequences in cooperation with EGR transcription factors.
Biological function summary

NAB1 functions as a critical regulator in cellular processes by forming a complex with EGR-1 also known as early growth response protein 1. This interaction allows NAB1 to influence the expression of several genes involved in cell differentiation proliferation and apoptosis. NAB1 ensures proper cellular responses to environmental stimuli by repressing gene transcription that EGR-1 activates.

Pathways

NAB1 frequently associates with the MAPK/ERK signaling pathway and the apoptotic pathway. In the MAPK/ERK pathway it operates alongside proteins like EGR-1 to influence cellular proliferation and differentiation. Meanwhile NAB1's role in the apoptotic pathway is related to its interaction with genes and proteins regulating cell death enabling cells to respond appropriately to stress signals.

NAB1 has been linked to conditions like cancer and neurodegenerative diseases. Aberrant expression or dysfunction of NAB1 along with changes in other proteins such as EGR-1 can lead to uncontrolled cell growth and transformation contributing to tumorigenesis. Additionally misregulation of NAB1 may play a part in neurodegenerative disorders by affecting neuron survival and death though further research is needed to fully understand these connections.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2.
See full target information NGFI-A-binding protein 1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Science signaling 17:eadg4422 PubMed38166031

2024

The induction of SHP-1 degradation by TAOK3 ensures the responsiveness of T cells to TCR stimulation.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandre Poirier,João Vitor Silva Ormonde,Isabelle Aubry,Belma Melda Abidin,Chu-Han Feng,Zuzet Martinez-Cordova,Ana Maria Hincapie,Chenyue Wu,Luis Alberto Pérez-Quintero,Chia-Lin Wang,Anne Claude Gingras,Joaquín Madrenas,Michel L Tremblay

Cancer management and research 13:4483-4495 PubMed34113174

2021

Ubenimex Suppresses the Ability of Migration and Invasion in Gastric Cancer Cells by Alleviating the Activity of the CD13/NAB1/MAPK Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xuehui Liu,Qie Guo,FanJing Jing,ChangKai Zhou,Ting Xiu,YunYan Shi,FanBo Jing
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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