AB112056

Anti-Nab2 antibody

Name: Anti-Nab2 antibody (ab112056) | Abcam
Brand: Abcam Limited
SKU: AB112056

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(1 Publication)

Rabbit Polyclonal NAB2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human NAB2 aa 450-550.

View Alternative Names

MADER, NAB2, NGFI-A-binding protein 2, EGR-1-binding protein 2, Melanoma-associated delayed early response protein, Protein MADER

2 Images

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Immunoprecipitation - Anti-Nab2 antibody (AB112056)

Immunoprecipitation of NAB2. Samples : 1 mg of whole cell lysate from 293T cells. NAB2 was detected with anti-NAB2 antibody NAB2 antibody (ab112056) at 1 μg/ml. Lane 1- NAB2 was also immunoprecipitated by rabbit anti-NAB2 antibody NAB2 antibody (ab112056) at 6 μg/mg lysate. Lane 2- NAB2 was immunoprecipitated by an rabbit anti-NAB2 antibody that recognizes an upstream epitope. Lane 3- Rabbit IgG control. Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-Nab2 antibody (ab112056)

Predicted band size: 56 kDa

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Western blot - Anti-Nab2 antibody (AB112056)

All lanes:

Western blot - Anti-Nab2 antibody (ab112056) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg/mL

Lane 2:

HeLa whole cell lysate at 15 µg/mL

Lane 3:

HeLa whole cell lysate at 5 µg/mL

Lane 4:

Jurkat whole cell lysate at 50 µg/mL

Predicted band size: 56 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human NAB2 aa 450-550. The exact immunogen used to generate this antibody is proprietary information.

Q15742

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Nab2 protein also known as Nab2p plays an important role in mRNA export and processing. It is identified as a nuclear poly(A) RNA-binding protein with a molecular mass of approximately 74 kDa. Nab2 is expressed primarily in the nucleus where it binds mRNA molecules participating in their proper transport from the nucleus to the cytoplasm. By attaching to the poly(A) tails of mRNA Nab2 ensures their stability and regulates nuclear export processes.

Biological function summary

The Nab2 protein is essential in maintaining RNA metabolism. Nab2 acts as a component of the mRNA processing machinery and is part of a complex involving other RNA-binding proteins. By interacting with poly(A) tails Nab2 influences the length and composition affecting mRNA stability and splicing. As a result this protein has an important function in normal cell growth and adaptation to environmental changes.

Pathways

Nab2 actively participates in RNA processing and export pathways. It works closely within the nucleo-cytoplasmic transport pathway to facilitate the correct mRNA distribution between the nucleus and cytoplasm. Nab2 associates with other proteins such as Gle1 and Mex67 forming a network that guides the mRNA transport through nuclear pore complexes. These interactions allow a coordinated process that ensures mRNAs reach their cytoplasmic sites effectively for translation.

Alterations in Nab2 function can impact cellular processes leading to neurodegeneration and other stress-related conditions. Disruption in mRNA processing and export related to Nab2 can contribute to neurological disorders due to the protein's role in neural cells. Moreover aberrant interactions with proteins like FMR1 have been implicated in conditions such as fragile X syndrome highlighting the importance of Nab2 in maintaining neuronal health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2. Isoform 2 lacks repression ability (By similarity).

See full target information NAB2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular and cellular biology 44:72-85 PubMed38482865

2024

ANP32e Binds Histone H2A.Z in a Cell Cycle-Dependent Manner and Regulates Its Protein Stability in the Cytoplasm.

Applications

Unspecified application

Species

Unspecified reactive species

Yasmin Dijkwel,Gene Hart-Smith,Sebastian Kurscheid,David J Tremethick

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Nab2 antibody

Immunoprecipitation - Anti-Nab2 antibody (AB112056)

Western blot - Anti-Nab2 antibody (AB112056)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise